Abstract:
:A multipurpose plasmid, pUR 250, has been constructed. It contains seven unique cloning sites (Hind III, Xba I, Sal I, Acc I, Hinc II, BamH I and EcoR I) in a small region of its lac Z gene part. Insertion of foreign DNA into the plasmid can be easily detected. Plasmid DNA isolated from a clone by a rapid method can be used to determine the DNA sequence of the insert from both ends directly without isolation of labeled fragment.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Rüther Udoi
10.1093/nar/10.19.5765subject
Has Abstractpub_date
1982-10-11 00:00:00pages
5765-72issue
19eissn
0305-1048issn
1362-4962journal_volume
10pub_type
杂志文章abstract::Giant chromatin-modifying complexes regulate gene transcription in eukaryotes by acting on chromatin substrates and 'setting' the histone code. The histone deacetylase (HDAC)-associated mammalian Sin3 corepressor complex regulates a wide variety of genes involved in all aspects of cellular physiology. The recruitment ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp051
更新日期:2009-04-01 00:00:00
abstract::RNase III enzymes cleave double stranded (ds)RNA. This is an essential step for regulating the processing of mRNA, rRNA, snoRNA and other small RNAs, including siRNA and miRNA. Arabidopsis thaliana encodes nine RNase III: four DICER-LIKE (DCL) and five RNASE THREE LIKE (RTL). To better understand the molecular functio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx820
更新日期:2017-11-16 00:00:00
abstract::The complete nucleotide sequences of the various precursor 5S RNA species occurring in Bacillus licheniformis have been elucidated. The B. licheniformis precursors contain a 5'-precursor-specific segment of 95 nucleotides which is four times as long as the corresponding segment of the p5S RNAs from the closely related...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.19.4535
更新日期:1980-10-10 00:00:00
abstract::alpha2-Macroglobulin covalently linked to poly(L)-lysine can be used as a vehicle for receptor-mediated gene transfer. This modified alpha2-macroglobulin maintains its ability to bind to the alpha2-macroglobulin receptor, and was shown to introduce a luciferase reporter gene plasmid into HepG2 human hepatoma cells in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.19.3873
更新日期:1996-10-01 00:00:00
abstract::Recent studies have shown that homologous recombination (HR) requires chromatin repression as well as relaxation at DNA double strand breaks (DSBs). HP1 and SUV39H1/2 are repressive factors essential for HR. Here, we identify SETDB1 as an additional compacting factor promoting HR. Depletion of HP1, SUV39, SETDB1 or BR...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv722
更新日期:2015-09-18 00:00:00
abstract::Guanine rich nucleic acid sequences can form G-quadruplex (G4) structures that interfere with DNA replication, repair and RNA transcription. The human FANCJ helicase contributes to maintaining genomic integrity by promoting DNA replication through G4-forming DNA regions. Here, we combined single-molecule and ensemble ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw574
更新日期:2016-10-14 00:00:00
abstract::The tight-binding I12-X86 lac repressor binds to non-operator DNA in a sequence-specific fashion. Using the DNA of the E. coli I gene we have investigated these sequence-specific interactions and compared them to the operator binding of wild-type repressor. The specific, non-operator DNA interactions are sensitive to ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.3.487
更新日期:1980-02-11 00:00:00
abstract::Pathbase is a database that stores images of the abnormal histology associated with spontaneous and induced mutations of both embryonic and adult mice including those produced by transgenesis, targeted mutagenesis and chemical mutagenesis. Images of normal mouse histology and strain-dependent background lesions are al...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh124
更新日期:2004-01-01 00:00:00
abstract::Copy number variants (CNVs) are currently defined as genomic sequences that are polymorphic in copy number and range in length from 1000 to several million base pairs. Among current array-based CNV detection platforms, long-oligonucleotide arrays promise the highest resolution. However, the performance of currently av...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn110
更新日期:2008-04-01 00:00:00
abstract::As an approach to define the structural features within the 5' noncoding region of Yellow Fever virus (YFV) that modulate mRNA translational efficiency, we have studied how minor changes in this region affect the translational capacity in vitro of the corresponding mRNAs. A cDNA sequence coding for part of the YFV str...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.7.2463
更新日期:1989-04-11 00:00:00
abstract::A new method is reported for the synthesis of oligodeoxyribonucleotides containing 2-aminopurine residues at selected sites. This method involves protection of the 2-aminopurine ribonucleoside, reduction to the deoxyribonucleoside and standard preparation of the 5'-0- (4,4'-dimethoxytrityl)-3'-O-(2-cyanoethyl)-N,N- di...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.4.754
更新日期:1996-02-15 00:00:00
abstract::We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.7.1521
更新日期:1991-04-11 00:00:00
abstract::The synthesis of ribosomal precursor RNA in Novikoff hepatoma (N1S1) cells is very sensitive to cordycepin (3'-dA). The synthesis of hnRNA, however, is resistant to inhibition concentrations of 3'-dA that completely block the synthesis of 45S ribosomal RNA precursor. We have examined the RNA polymerases present in the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.2.325
更新日期:1976-02-01 00:00:00
abstract::Adaptation to the host cell environment to efficiently take-over the host cell's machinery is crucial in particular for small RNA viruses like picornaviruses that come with only small RNA genomes and replicate exclusively in the cytosol. Their Internal Ribosome Entry Site (IRES) elements are specific RNA structures th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks182
更新日期:2012-07-01 00:00:00
abstract::A-tracts are functionally important DNA sequences which induce helix bending and have peculiar structural properties. While A-tract structure has been qualitatively well characterized, their mechanical properties remain controversial. A-tracts appear structurally rigid and resist nucleosome formation, but seem flexibl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku338
更新日期:2014-06-01 00:00:00
abstract::Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes conformational changes resulting in DNA strand separation to form a transcription bubble and ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1146
更新日期:2018-01-25 00:00:00
abstract::Nucleotide substitution, insertion and deletion (indel) events are the major driving forces that have shaped genomes. Using the recently identified human ribosomal protein (RP) pseudogene sequences, we have thoroughly studied DNA mutation patterns in the human genome. We analyzed a total of 1726 processed RP pseudogen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg745
更新日期:2003-09-15 00:00:00
abstract::Hidden Markov models (HMMs) and their variants are widely used in Bioinformatics applications that analyze and compare biological sequences. Designing a novel application requires the insight of a human expert to define the model's architecture. The implementation of prediction algorithms and algorithms to train the m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp662
更新日期:2009-11-01 00:00:00
abstract::By subtractive screening of a library made from mRNA of lipopolysaccharide (LPS)-stimulated mouse B lymphocytes we isolated cDNA-clones encoding the ribosomal protein L7. Human L7 mRNA was cloned from activated T-lymphocytes. Although no specific function of L7 in the translation apparatus is known as yet, it should b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.2.223
更新日期:1993-01-25 00:00:00
abstract::Highly conserved sequences present at an identical position near the 3' ends of eukaryotic and prokaryotic 5S rRNAs are complementary to the 5' strand of the m2(6)A hairpin structure near the 3' ends of 18S rRNA and 16S rRNA, respectively. The extent of base-pairing and the calculated stabilities of the hybrids that c...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/7.7.1913
更新日期:1979-12-11 00:00:00
abstract::enod40 is a plant gene that participates in the regulation of symbiotic interaction between leguminous plants and bacteria or fungi. Furthermore, it has been suggested to play a general role in non-symbiotic plant development. Although enod40 seems to have multiple functions, being present in many land plants, the mol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm173
更新日期:2007-01-01 00:00:00
abstract::Understanding networks of protein-protein interactions constitutes an essential component on a path towards comprehensive description of cell function. Whereas efficient techniques are readily available for the initial identification of interacting protein partners, practical strategies are lacking for the subsequent ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm563
更新日期:2007-01-01 00:00:00
abstract::We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the human papillomavirus E6...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn903
更新日期:2009-01-01 00:00:00
abstract::We have identified and sequenced two members of a chicken middle repetitive DNA sequence family. By reassociation kinetics, members of this family (termed CRl) are estimated to be present in 1500-7000 copies per chicken haploid genome. The first family member sequenced (CRlUla) is located approximately 2 kb upstream f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.20.5383
更新日期:1981-10-24 00:00:00
abstract::Coralyne is a small crescent-shaped molecule known to intercalate duplex and triplex DNA. We report that coralyne can cause the complete and irreversible disproportionation of duplex poly(dT)*poly(dA). That is, coralyne causes the strands of duplex poly(dT)*poly(dA) to repartition into equal molar equivalents of tripl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.4.983
更新日期:2002-02-15 00:00:00
abstract::PKR is a doubled-stranded RNA-dependent protein kinase which is implicated in the regulation of several cellular processes, including cell proliferation. PKR undergoes phosphorylation and activation in mouse embryonic 3T3-F442A cells in response to endogenous RNA(s). Activation of PKR is related to growth and differen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.13.2672
更新日期:1997-07-01 00:00:00
abstract::Transcription initiation complexes formed by bacterial RNA polymerases (RNAPs) exhibit dramatic species-specific differences in stability, leading to different strategies of transcription regulation. The molecular basis for this diversity is unclear. Promoter complexes formed by RNAP from Thermus aquaticus (Taq) are c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks973
更新日期:2012-12-01 00:00:00
abstract::The polymerase encoded by human hepatitis B virus, which has reverse transcriptase and RNase H activity, binds to its pregenomic RNA template in a two-step process involving a terminal redundancy. Both first strand and second strand DNA synthesis involve primer translocation and second strand synthesis involves a temp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.17.3295
更新日期:1996-09-01 00:00:00
abstract::To generate long arrays of nucleosomes within a topologically defined chromatin domain we have assembled minichromosomes on negatively supercoiled plasmid DNA with extracts from Drosophila preblastoderm embryos. These minichromosomes are dynamic substrates for energy-dependent nucleosome remodeling machines that facil...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.16.3657
更新日期:1998-08-15 00:00:00
abstract::In the past year, DDBJ (http://www.ddbj.nig.ac.jp) collected and released 1,956,826 entries or 1,741,313,111 bases. The released data include approximately 90,000 ESTs and cDNAs of Macaca fascicularis, and 280 million bases of mouse GSS. In addition to the data collection, we have indexed the submitted data to the Int...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj111
更新日期:2006-01-01 00:00:00