The sensitivity of RNA polymerases I and II from Novikoff hepatoma (N1S1) cells to 3'-deoxyadenosine 5'-triphosphate.

abstract：:Most small nucleolar RNAs (snoRNAs) guide rRNA nucleotide modifications, some participate in pre-rRNA cleavages, and a few have both functions. These activities involve direct base-pairing of the snoRNA with pre-rRNA using different domains. It is not known if the modification and processing functions occur independen...

journal_title：Nucleic acids research

authors： Liang XH,Liu Q,Liu Q,King TH,Fournier MJ

更新日期：2010-06-01 00:00:00

abstract：:Many nucleic acid-binding proteins and the AAA+ family form hexameric rings, but the mechanism of hexamer assembly is unclear. It is generally believed that the specificity in protein/RNA interaction relies on molecular contact through a surface charge or 3D structure matching via conformational capture or induced fit...

journal_title：Nucleic acids research

authors： Xiao F,Zhang H,Guo P

更新日期：2008-11-01 00:00:00

abstract：:8-Oxo-7,8-dihydroguanine (8-oxoGua) is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The MutT protein of Escherichia col...

journal_title：Nucleic acids research

authors： Ishibashi T,Hayakawa H,Ito R,Miyazawa M,Yamagata Y,Sekiguchi M

更新日期：2005-07-07 00:00:00

abstract：:Detailed models are presented for the triple-stranded polynucleotide helices of poly (U)-poly (A)-poly (U) (two forms), poly (U)-poly d (A) -poly (U), poly d(C)-poly d(I)-poly d(C), poly d(T)-polyd(A)-poly d(T) and poly (I)-poly (A)-poly (I). The models were genrated using a computerized, linked-atom procedure which p...

journal_title：Nucleic acids research

authors： Arnott S,Bond PJ,Selsing E,Smith PJ

更新日期：1976-10-01 00:00:00

abstract：:Alpha-1-microglobulin and bikunin are two plasma glycoproteins encoded by an alpha-1-microglobulin/bikunin precursor (AMBP) gene. The strict liver-specific expression of the AMBP gene is controlled by a potent enhancer made of six clustered boxes numbered 1-6 that have been reported to be proven or potential binding s...

journal_title：Nucleic acids research

authors： Rouet P,Raguenez G,Tronche F,Mfou'ou V,Salier JP

更新日期：1995-02-11 00:00:00

abstract：:We have investigated the attachment of the DNA to the nuclear matrix during the division cycle of the plasmodial slime mold Physarum polycephalum. The DNA of plasmodia was pulse labelled at different times during the S phase and the label distribution was studied by graded DNase digestion of the matrix-DNA complexes p...

journal_title：Nucleic acids research

authors： Aelen JM,Opstelten RJ,Wanka F

更新日期：1983-02-25 00:00:00

abstract：:Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguan...

journal_title：Nucleic acids research

authors： Agris PF,Schmidt PG

更新日期：1980-05-10 00:00:00

abstract：:We describe here an energy based computer software suite for narrowing down the search space of tertiary structures of small globular proteins. The protocol comprises eight different computational modules that form an automated pipeline. It combines physics based potentials with biophysical filters to arrive at 10 pla...

journal_title：Nucleic acids research

authors： Jayaram B,Bhushan K,Shenoy SR,Narang P,Bose S,Agrawal P,Sahu D,Pandey V

更新日期：2006-01-01 00:00:00

abstract：:Recent rapid advances in next generation RNA sequencing (RNA-Seq)-based provide researchers with unprecedentedly large data sets and open new perspectives in transcriptomics. Furthermore, RNA-Seq-based transcript profiling can be applied to non-model and newly discovered organisms because it does not require a predefi...

journal_title：Nucleic acids research

authors： Lohse M,Bolger AM,Nagel A,Fernie AR,Lunn JE,Stitt M,Usadel B

更新日期：2012-07-01 00:00:00

abstract：:The modulation of GLI2, an oncogenic transcription factor commonly upregulated in cancer, is in many cases not due to genetic defects, suggesting dysregulation through alternative mechanisms. The identity of these molecular events remains for the most part unknown. Here, we identified TFII-I as a novel repressor of GL...

journal_title：Nucleic acids research

authors： McCleary-Wheeler AL,Paradise BD,Almada LL,Carlson AJ,Marks DL,Vrabel A,Vera RE,Sigafoos AN,Olson RL,Fernandez-Zapico ME

更新日期：2020-07-27 00:00:00

abstract：:The DNA repair enzyme human uracil DNA glycosylase (UNG) scans short stretches of genomic DNA and captures rare uracil bases as they transiently emerge from the DNA duplex via spontaneous base pair breathing motions. The process of DNA scanning requires that the enzyme transiently loosen its grip on DNA to allow stoch...

journal_title：Nucleic acids research

authors： Friedman JI,Majumdar A,Stivers JT

更新日期：2009-06-01 00:00:00

abstract：:Nucleotide excision repair (NER) is a major DNA repair pathway for a variety of DNA lesions. XPB plays a key role in DNA opening at damage sites and coordinating damage incision by nucleases. XPB is conserved from archaea to human. In archaea, XPB is associated with a nuclease Bax1. Here we report crystal structures o...

journal_title：Nucleic acids research

authors： DuPrez K,He F,Chen Z,Hilario E,Fan L

更新日期：2020-06-19 00:00:00

abstract：:The T box transcription antitermination regulatory system, found in Gram-positive bacteria, is dependent on a complex set of interactions between uncharged tRNA and the 5'-untranslated mRNA leader region of the regulated gene. One of these interactions involves the base pairing of the acceptor end of cognate tRNA with...

journal_title：Nucleic acids research

authors： Fauzi H,Jack KD,Hines JV

更新日期：2005-05-06 00:00:00

abstract：:A growing body of evidence suggests that siRNA could generate off-target effects through different mechanisms. However, the full impact of off-target gene regulation on phenotypic induction and accordingly on data interpretation in the context of large-scale siRNA library screen has not been reported. Here we report o...

journal_title：Nucleic acids research

authors： Lin X,Ruan X,Anderson MG,McDowell JA,Kroeger PE,Fesik SW,Shen Y

更新日期：2005-08-09 00:00:00

abstract：:Two of the tRNA's found in rabbit reticulocytes are substrates for a post-transcriptional modification leading to the incorporation of guanine into the polynucleotide chain. The major guanylated tRNA was previously identified as tRNA (His). In the present report we show that the minor guanylated tRNA is tRNA (Asn), an...

journal_title：Nucleic acids research

authors： Farkas WR,Chernoff D

更新日期：1976-10-01 00:00:00

abstract：:We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresp...

journal_title：Nucleic acids research

authors： Mergny JL,Collier D,Rougée M,Montenay-Garestier T,Hélène C

更新日期：1991-04-11 00:00:00

abstract：:Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a relatively big size, imperfect specificity and restriction to DNA targets ...

journal_title：Nucleic acids research

authors： Fedorova I,Arseniev A,Selkova P,Pobegalov G,Goryanin I,Vasileva A,Musharova O,Abramova M,Kazalov M,Zyubko T,Artamonova T,Artamonova D,Shmakov S,Khodorkovskii M,Severinov K

更新日期：2020-02-28 00:00:00

abstract：:Emerging evidence suggests that recombinant adeno-associated viral (rAAV) vectors can be used for specific gene targeting in human somatic cells. We have developed an rAAV vector construction procedure employing fusion PCR and a single cloning step that considerably simplifies the knockout process. We demonstrate its ...

journal_title：Nucleic acids research

authors： Kohli M,Rago C,Lengauer C,Kinzler KW,Vogelstein B

更新日期：2004-01-02 00:00:00

abstract：:To achieve drug resistance Leishmania parasite alters gene copy number by using its repeated sequences widely distributed through the genome. Even though homologous recombination (HR) is ascribed to maintain genome stability, this eukaryote exploits this potent mechanism driven by the Rad51 recombinase to form benefic...

journal_title：Nucleic acids research

authors： Genois MM,Plourde M,Éthier C,Roy G,Poirier GG,Ouellette M,Masson JY

更新日期：2015-03-11 00:00:00

abstract：:Elucidating the dynamic organization of nuclear RNA foci is important for understanding and manipulating these functional sites of gene expression in both physiological and pathological states. However, such studies have been difficult to establish in vivo as a result of the absence of suitable RNA imaging methods. He...

journal_title：Nucleic acids research

authors： Oomoto I,Suzuki-Hirano A,Umeshima H,Han YW,Yanagisawa H,Carlton P,Harada Y,Kengaku M,Okamoto A,Shimogori T,Wang DO

更新日期：2015-10-30 00:00:00

abstract：:In kinetoplastid protozoa, mitochondrial (mt) mRNAs are post-transcriptionally edited by insertion and deletion of uridylate residues, the information being provided by guide (g)RNAs. Currently popular mechanisms for the editing process envisage a series of consecutive 'cut-and-paste' reactions, carried out by a compl...

journal_title：Nucleic acids research

authors： Blom D,Burg Jv,Breek CK,Speijer D,Muijsers AO,Benne R

更新日期：2001-07-15 00:00:00

abstract：:A formaldehyde denaturation map of the replicative form of phiX174 DNA is obtained. The RFI DNA was converted into a linear state by restriction endonuclease pst I which introduces into this DNA a single double-stranded break. The map has four clear-cut peaks. Their positions excellently correlate with the peak positi...

journal_title：Nucleic acids research

authors： Karataev GI,Permogorov VI,Vologodskii AV,Frank-Kamenetskii MD

更新日期：1978-07-01 00:00:00

abstract：:An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying genomic DNA templa...

journal_title：Nucleic acids research

authors： Ponelies N,Stein N,Weber G

更新日期：1997-09-01 00:00:00

abstract：:The database of imprinted genes and parent-of-origin effects in animals (http://www.otago.ac.nz/IGC ) is a collation of genes and phenotypes for which parent-of-origin effects have been reported. The database currently includes over 220 entries, which describe over 40 imprinted genes in human, mouse and other animals....

journal_title：Nucleic acids research

authors： Morison IM,Paton CJ,Cleverley SD

更新日期：2001-01-01 00:00:00

abstract：:We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitiv...

journal_title：Nucleic acids research

authors： Hartig A,Simon MM,Schuster T,Daugherty JR,Yoo HS,Cooper TG

更新日期：1992-11-11 00:00:00

abstract：:The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp contr...

journal_title：Nucleic acids research

authors： Caughey PA,de Feyter R,Lane HE

更新日期：1986-12-22 00:00:00

abstract：:The abundance and complexity of total poly(A)+RNA of human normal unstimulated lymphocytes, PHA-stimulated and leukemic lymphocytes were examined using the cDNA-poly(A)+ RNA hybridization technique. It was found that in both PHA-stimulated and leukemic lymphocytes the complexity of total poly(A)+RNA is strongly reduce...

journal_title：Nucleic acids research

authors： Torelli G,Ferrari S,Donelli A,Cadossi R,Ferrari S,Bosi P,Torelli U

更新日期：1981-12-21 00:00:00

abstract：:In Xenopus oocytes, progesterone stimulates the cytoplasmic polyadenylation and resulting translational activation of c-mos mRNA, which is necessary for the induction of oocyte maturation. Although details of the biochemistry of polyadenylation are beginning to emerge, the mechanism by which 3' poly(A) addition stimul...

journal_title：Nucleic acids research

authors： Kuge H,Brownlee GG,Gershon PD,Richter JD

更新日期：1998-07-01 00:00:00

abstract：:Release of phosphorothioate antisense oligonucleotides (PS-ASOs) from late endosomes (LEs) is a rate-limiting step and a poorly defined process for productive intracellular ASO drug delivery. Here, we examined the role of Golgi-endosome transport, specifically M6PR shuttling mediated by GCC2, in PS-ASO trafficking and...

journal_title：Nucleic acids research

authors： Liang XH,Sun H,Hsu CW,Nichols JG,Vickers TA,De Hoyos CL,Crooke ST

更新日期：2020-02-20 00:00:00

abstract：:Large expansions of the trinucleotide repeat GAA*TTC within the first intron of the X25 (frataxin) gene cause Friedreich's ataxia, the most common inherited ataxia. Expansion leads to reduced levels of frataxin mRNA in affected individuals. Here we show that GAA*TTC tracts, in the absence of any other frataxin gene se...

journal_title：Nucleic acids research

authors： Grabczyk E,Usdin K

更新日期：2000-07-15 00:00:00