Abstract：
:Several derivatives of N2,N2-7-trimethylguanosine (m3(2,2,7G)-cap, which was found at the 5' ends of small nuclear RNAs, were synthesized by use of S-phenyl N2,N2,7-trimethylguanosine 5'-phosphorothioate (PhSpm3(2,2,7)G) as a key intermediate. This compound was activated by iodine in the presence of phosphoric acid and diphosphoric acid to give N2,N2,7-trimethylguanosine-5'-diphosphate (ppm3(2,2,7)G) and 5'-triphosphate (ppm3(2,2,7)G), respectively. Similar reactions of PhSpm3(2,2,7)G with ADP and GDP gave capped dinucleoside triphosphates, m3(2,2,7)G5'pppA and m3(2,2,7)G5'pppG, respectively.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Iwase R,Sekine M,Tokumoto Y,Ohshima Y,Hata Tdoi
10.1093/nar/17.22.8979subject
Has Abstractpub_date
1989-11-25 00:00:00pages
8979-89issue
22eissn
0305-1048issn
1362-4962journal_volume
17pub_type
杂志文章abstract：:Initially identified as a factor involved in tyrosine kinase receptor signaling, Grb10-interacting GYF protein 2 (GIGYF2) has later been shown to interact with the 5' cap-binding protein 4EHP as part of a translation repression complex, and to mediate post-transcriptional repression of tethered reporter mRNAs. A curre...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky198
更新日期：2018-06-20 00:00:00
abstract：:The unsymmetrical cyanine dye thiazole orange homodimer (TOTO) binds to single-stranded DNA (ssDNA, M13mp18 ssDNA) to form a fluorescent complex that is stable under the standard conditions of electrophoresis. The stability of this complex is indistinguishable from that of the corresponding complex of TOTO with double...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.7.1215
更新日期：1995-04-11 00:00:00
abstract：:The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. Genome wide localization studies have demonstrated that Mediator occupancy not only correlates with high levels of transcription, but that the complex also is present at transcriptionally silence...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr551
更新日期：2011-10-01 00:00:00
abstract：:We have developed an effective, easy-to-use two-step system for the site-directed insertion of large genetic constructs into arbitrary positions in the Escherichia coli chromosome. The system uses lambda-Red mediated recombineering accompanied by the introduction of double-strand DNA breaks in the chromosome and a don...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1193
更新日期：2010-04-01 00:00:00
abstract：:Ribosomal protein (rp)S5 belongs to the family of the highly conserved rp's that contains rpS7 from prokaryotes and rpS5 from eukaryotes. Alignment of rpS5/rpS7 from metazoans (Homo sapiens), fungi (Saccharomyces cerevisiae) and bacteria (Escherichia coli) shows that the proteins contain a conserved central/C-terminal...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1113
更新日期：2010-03-01 00:00:00
abstract：:The gene encoding DNA polymerase alpha from the human malaria parasite Plasmodium falciparum has been sequenced and characterised. The deduced amino acid sequence possesses the seven sequence motifs which characterise eukaryotic replicative DNA polymerases (I-VII) and four of five motifs (A-E) identified in alpha DNA ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.16.3643
更新日期：1993-08-11 00:00:00
abstract：:The purpose of this work was to analyse at the molecular level the DNA recognized by the antibodies to Z-DNA in in situ experiments. Antibodies to Z-DNA interact strongly with R-band positive heterochromatic segments of fixed metaphase chromosomes of Cebus (Viegas-Pequignot et al., 1983). These segments are constitute...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.8.3197
更新日期：1986-04-25 00:00:00
abstract：:Bacteria core RNA polymerase (RNAP) must associate with a sigma factor to recognize promoter sequences. Promoters recognized by the sigma(54) (or sigma(N)) associated RNA polymerase are unique in having conserved positions around -24 and -12 nucleotides upstream from the transcriptional start site. Using DNA microarra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1123
更新日期：2010-03-01 00:00:00
abstract：:The National Center for Biotechnology Information (NCBI) provides a large suite of online resources for biological information and data, including the GenBank® nucleic acid sequence database and the PubMed database of citations and abstracts for published life science journals. The Entrez system provides search and re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1095
更新日期：2018-01-04 00:00:00
abstract：:The adenovirus late region 1 (L1) represents an example of an alternatively spliced gene where one 5' splice site is spliced to two alternative 3' splice sites, to produce two mRNAs; the 52,55K and IIIa mRNAs, respectively. Accumulation of the L1 mRNAs is temporally regulated during the infectious cycle. Thus, the pro...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.3.332
更新日期：1994-02-11 00:00:00
abstract：:We describe conditions for producing uninterrupted expanded CTG repeats consisting of up to 2000 repeats using 29 DNA polymerase. Previously, generation of such repeats was hindered by CTG repeat instability in plasmid vectors maintained in Escherichia coli and poor in vitro ligation of CTG repeat concatemers due to s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn025
更新日期：2008-03-01 00:00:00
abstract：:On the tandemly linked ribosomal genes of Xenopus laevis, the RNA polymerase transcribes past the 3' end of the 40S coding region and terminates at T3 just upstream of the gene promoter. The close proximity of T3 to the gene promoter, and the functional interdependence of these two elements, has led to the suggestion ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.1.373
更新日期：1989-01-11 00:00:00
abstract：:The nucleobase modification 5-methylcytosine (m(5)C) is widespread both in DNA and different cellular RNAs. The functions and enzymatic mechanisms of DNA m(5)C-methylation were extensively studied during the last decades. However, the location, the mechanism of formation and the cellular function(s) of the same modifi...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkp1117
更新日期：2010-03-01 00:00:00
abstract：:Large-scale sequencing studies discovered substantial genetic variants occurring in enhancers which regulate genes via long range chromatin interactions. Importantly, such variants could affect enhancer regulation by changing transcription factor bindings or enhancer hijacking, and in turn, make an essential contribut...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx920
更新日期：2018-01-04 00:00:00
abstract：:In vitro compartmentalization (IVC) was employed for the first time to select for novel bacteriophage lambda integrase variants displaying significantly enhanced recombination activity on a non-cognate target DNA sequence. These variants displayed up to 9-fold increased recombination activity over the parental enzyme,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1089
更新日期：2010-03-01 00:00:00
abstract：:Although subtelomeric regions in humans are heterochromatic, the epigenetic nature of human telomeres remains controversial. This controversy might have been influenced by the confounding effect of subtelomeric regions and interstitial telomeric sequences (ITSs) on telomeric chromatin structure analyses. In addition, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky006
更新日期：2018-03-16 00:00:00
abstract：:Previous studies have shown that the yeast Candida albicans encodes a unique seryl-tRNA(CAG) that should decode the leucine codon CUG as serine. However, in vitro translation of several different CUG-containing mRNAs in the presence of this unusual seryl-tRNA(CAG) result in an apparent increase in the molecular weight...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.9.1481
更新日期：1995-05-11 00:00:00
abstract：:Our database on large ribosomal subunit RNA contained 334 sequences in July, 1995. All sequences in the database are aligned, taking into account secondary structure. The aligned sequences are provided, together with incorporated secondary structure information, in several computer-readable formats. These data can eas...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.1.92
更新日期：1996-01-01 00:00:00
abstract：:The KEGG RPAIR database is a collection of biochemical structure transformation patterns, called RDM patterns, and chemical structure alignments of substrate-product pairs (reactant pairs) in all known enzyme-catalyzed reactions taken from the Enzyme Nomenclature and the KEGG PATHWAY database. Here, we present PathPre...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq318
更新日期：2010-07-01 00:00:00
abstract：:We have previously shown that in vivo estradiol-dependent dephosphorylation of MDBP-2-H1 (a member of the histone H1 family) correlates with the loss of in vitro preferential binding to methylated DNA. To study the effects of the phosphorylation/dephosphorylation of MDBP-2-H1 on the expression of the avian vitellogeni...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.10.1816
更新日期：1996-05-15 00:00:00
abstract：:Multivalent cations are known to condense DNA into higher ordered structures, including toroids and rods. Here we report that solid supports treated with monovalent or multivalent cationic silanes, followed by removal of soluble molecules, can condense DNA. The mechanism of this surface-directed condensation depends o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.2.588
更新日期：1998-01-15 00:00:00
abstract：:Antigenic variation of the pilus is an essential component of Neisseria gonorrhoeae pathogenesis. Unidirectional recombination of silent pilin DNA into an expressed pilin gene allows for substantial sequence variation of this highly immunogenic surface structure. While the RecA protein is required for pilin gene recom...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.7.1362
更新日期：1997-04-01 00:00:00
abstract：:A coiled-coil microtubule-bundling protein, p180, was originally identified as one of the ribosome receptor candidates on the rough endoplasmic reticulum (ER) and is highly expressed in secretory tissues. Recently, we reported that p180 plays crucial roles in upregulating collagen biosynthesis, mainly by facilitating ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1197
更新日期：2012-04-01 00:00:00
abstract：:A pan-genome is the union of the gene sets of all the individuals of a clade or a species and it provides a new dimension of genome complexity with the presence/absence variations (PAVs) of genes among these genomes. With the progress of sequencing technologies, pan-genome study is becoming affordable for eukaryotes w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw958
更新日期：2017-01-25 00:00:00
abstract：:By successively assembling genetic parts such as BioBrick according to grammatical models, complex genetic constructs composed of dozens of functional blocks can be built. However, usually every category of genetic parts includes a few or many parts. With increasing quantity of genetic parts, the process of assembling...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx859
更新日期：2017-12-15 00:00:00
abstract：:Acetyl-CoA Carboxylase catalyzes the first committed step in fatty acid synthesis. Escherichia coli acetyl-CoA carboxylase is composed of biotin carboxylase, carboxyltransferase and biotin carboxyl carrier protein functions. The accA and accD genes that code for the alpha- and beta-subunits, respectively, are not in a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp1079
更新日期：2010-03-01 00:00:00
abstract：:African trypanosomes are extracellular protozoan parasites that are transmitted from one mammalian host to the next by tsetse flies. Bloodstream forms express variant surface glycoprotein (VSG); the tsetse fly (procyclic) forms express instead the procyclic acidic repetitive protein (PARP). PARP mRNA is abundant in pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.7.1202
更新日期：1996-04-01 00:00:00
abstract：:High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency along with a higher propensity to cause hepatotoxicity. In order to understand the mechanism of this hepatotoxicity, t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1210
更新日期：2016-03-18 00:00:00
abstract：:Human adenovirus mutants that carry a large deletion in early region 4 (E4) are severely defective in the synthesis of viral late proteins. Plasmids that carry intact E4 sequences can complement the late protein synthetic defect of such mutants when introduced into infected cells by transfection, presumably due to the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.8.3037
更新日期：1989-04-25 00:00:00
abstract：:In Escherichia coli, the MnmEG complex modifies transfer RNAs (tRNAs) decoding NNA/NNG codons. MnmEG catalyzes two different modification reactions, which add an aminomethyl (nm) or carboxymethylaminomethyl (cmnm) group to position 5 of the anticodon wobble uridine using ammonium or glycine, respectively. In tRNA(cmnm...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1228
更新日期：2014-02-01 00:00:00
