Abstract:
:Our database on large ribosomal subunit RNA contained 334 sequences in July, 1995. All sequences in the database are aligned, taking into account secondary structure. The aligned sequences are provided, together with incorporated secondary structure information, in several computer-readable formats. These data can easily be obtained through the World Wide Web. The files in the database are also available via anonymous ftp.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
De Rijk P,Van de Peer Y,De Wachter Rdoi
10.1093/nar/24.1.92subject
Has Abstractpub_date
1996-01-01 00:00:00pages
92-7issue
1eissn
0305-1048issn
1362-4962pii
5s1026journal_volume
24pub_type
杂志文章abstract::RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.23.7763
更新日期:1982-12-11 00:00:00
abstract::The T box transcription antitermination regulatory system, found in Gram-positive bacteria, is dependent on a complex set of interactions between uncharged tRNA and the 5'-untranslated mRNA leader region of the regulated gene. One of these interactions involves the base pairing of the acceptor end of cognate tRNA with...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki546
更新日期:2005-05-06 00:00:00
abstract::Recurrent chromosome aberrations are an important resource when associating human pathologies to specific genes. However, for technical reasons a large number of chromosome breakpoints are defined only at the level of cytobands and many of the genes involved remain unidentified. We developed a web-based information sy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki061
更新日期:2005-01-01 00:00:00
abstract::RNase P is primarily responsible for the 5΄ maturation of transfer RNAs (tRNAs) in all domains of life. Archaeal RNase P is a ribonucleoprotein made up of one catalytic RNA and five protein cofactors including L7Ae, which is known to bind the kink-turn (K-turn), an RNA structural element that causes axial bending. How...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx388
更新日期:2017-07-07 00:00:00
abstract::Pyrimidine adducts in cellular DNA arise from modification of the pyrimidine 5,6-double bond by oxidation, reduction or hydration. The biological outcome includes increased mutation rate and potential lethality. A major DNA N:-glycosylase responsible for the excision of modified pyrimidine bases is the base excision r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.3.604
更新日期:2001-02-01 00:00:00
abstract::Besides the nicking-closing (topoisomerase I) activity, an ATP-dependent DNA topoisomerase is present in rat liver nuclei. The enzyme, partially purified, is able to catenate in vitro closed DNA circles in a magnesium-dependent, ATP-dependent, histone H1-dependent reaction, and to decatenate in vitro kinetoplast DNA n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.4.1059
更新日期:1983-02-25 00:00:00
abstract::The Kluyveromyces lactis gene encoding the general transcription factor IIB (TFIIB) was isolated from a genomic library by complementation of the cold-sensitive phenotype conferred by a mutation in the SUA7 gene, which encodes TFIIB in Saccharomyces cerevisiae. DNA sequence analysis of the KI-SUA7 gene revealed a 357 ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.15.3413
更新日期:1993-07-25 00:00:00
abstract::The results of differential expression analyses provide scientists with hundreds to thousands of differentially expressed genes that need to be interpreted in light of the biology of the specific system under study. This requires mapping the genes to functional classifications that can be, for example, the KEGG pathwa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt516
更新日期:2013-07-01 00:00:00
abstract::Eukaryotic gene expression is regulated by enhancer-promoter interactions but the molecular mechanisms that govern specificity have remained elusive. Genome-wide studies utilizing STARR-seq identified two enhancer classes in Drosophila that interact with different core promoters: housekeeping enhancers (hkCP) and deve...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1114
更新日期:2017-02-28 00:00:00
abstract::In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in post-transcriptional gene regulation. The ribonuclease activity of deadenylase enzymes is attributed to either a DEDD (Asp-Glu-Asp-Asp) or an endonuclease-exonuclease-phosphatase domain....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt972
更新日期:2014-03-01 00:00:00
abstract::The distribution patterns of shared short repetitive motifs in the expansion segments of the large subunit rRNA genes of different species show that these segments are coevolving as a set and that in two examined vertebrate species the RNA secondary structures are conserved as a consequence of runs of motifs in one re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.20.5949
更新日期:1990-10-25 00:00:00
abstract::ModBase (http://salilab.org/modbase) is a database of annotated comparative protein structure models. The models are calculated by ModPipe, an automated modeling pipeline that relies primarily on Modeller for fold assignment, sequence-structure alignment, model building and model assessment (http://salilab.org/modelle...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1091
更新日期:2011-01-01 00:00:00
abstract::We have isolated and sequenced a cDNA clone coding for human Retinol Binding Protein. The sequence indicates that Retinol Binding Protein is synthesized as a single polypeptide chain precursor which is then matured to the secreted protein by removal of a leader peptide. Southern and Northern blot analysis suggest that...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.22.7769
更新日期:1983-11-25 00:00:00
abstract::A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtaine...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.9.2103
更新日期:1993-05-11 00:00:00
abstract::Infection of cultured cells with herpes simplex virus (HSV) results in the transcriptional induction of a small number of cellular genes. Although the majority of such genes are dependent upon viral protein synthesis for their induction, a small minority are not. These genes are induced by events occurring prior to th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.23.9261
更新日期:1986-12-09 00:00:00
abstract::During erythropoiesis in the chick, the nucleosome repeat length increases from 190 base pairs to 212 base pairs. This increase is correlated with a dramatic increase in the concentration of the red cell specific histone H5 (from 0.2 molecules per nucleosome to 1 molecule per nucleosome) and with no change in the conc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.4.1179
更新日期:1978-04-01 00:00:00
abstract::Synthetic deoxyoligonucleotides have been 5'-aminoalkylated at the end of step-wise synthesis on the polymer support. This was achieved through the activation of the 5'-hydroxyl group as its 5'-imidazolyl derivative using carbonyldiimidazole, which was subsequently displaced with hexamethylene diamine to yield the tit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.20.7985
更新日期:1986-10-24 00:00:00
abstract::Biological evolution resulted in a homochiral world in which nucleic acids consist exclusively of d-nucleotides and proteins made by ribosomal translation of l-amino acids. From the perspective of synthetic biology, however, particularly anabolic enzymes that could build the mirror-image counterparts of biological mac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx079
更新日期:2017-04-20 00:00:00
abstract::Nucleotide excision repair (NER) mechanism is the major pathway responsible for the removal of a large variety of bulky lesions from the genome. Two different NER subpathways have been identified, i.e. the transcription-coupled and the global genome repair pathways. For DNA-damage induced by ultraviolet light both tra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.23.4653
更新日期:1996-12-01 00:00:00
abstract::The DNA sequences at and around the junctions between viral and cellular DNA in the polyoma virus transformed mouse cell line, TS-A-3T3, have been determined. No common sequence specificity or structural features at the joins have been observed. The sequence indicates that the 94K truncated large T antigen found in TS...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.15.4515
更新日期:1982-08-11 00:00:00
abstract::The expression of thymidine kinase in fungi, which normally lack this enzyme, will greatly aid the study of DNA metabolism and provide useful drug-sensitive phenotypes. The herpes simplex virus type-1 thymidine kinase gene ( tk ) was expressed in Neurospora crassa. tk was expressed as a fusion to N.crassa arg-2 regula...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.12.2389
更新日期:1997-06-15 00:00:00
abstract::Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studie...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv373
更新日期:2015-05-26 00:00:00
abstract::The structure of a ribonuclease III processing signal from bacteriophage T7 was examined by NMR spectroscopy, optical melting, and chemical and enzymatic modification. A 41 nucleotide variant of the T7 R1.1 processing signal has two Watson-Crick base-paired helices separated by an internal loop, consistent with its pr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.4.604
更新日期:1994-02-25 00:00:00
abstract::We have shown previously that DNA demethylation by chick embryo 5-methylcytosine (5-MeC)-DNA glycosylase needs both protein and RNA. Amino acid sequences of nine peptides derived from a highly purified 5-MeC-DNA glycosylase complex were identified by Nanoelectrospray ionisation mass spectrometry to be identical to the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.16.3245
更新日期:1999-08-15 00:00:00
abstract::Small interfering RNAs (siRNAs) are promising new active compounds in gene medicine but the induction of non-specific immune responses following their delivery continues to be a serious problem. With the purpose of avoiding such effects chemically modified siRNAs are tested in screening assay but often only examining ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks033
更新日期:2012-05-01 00:00:00
abstract::The mission of UniProt is to support biological research by providing a freely accessible, stable, comprehensive, fully classified, richly and accurately annotated protein sequence knowledgebase, with extensive cross-references and querying interfaces. UniProt is comprised of four major components, each optimized for ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr981
更新日期:2012-01-01 00:00:00
abstract::Analysis of 41 histone homologous clones from an isogenic gene library of Drosophila melanogaster showed that non-histone fragments interrupt the histone repetitive clusters at several sites. Long (L) and short (S) forms of the repeating units are distinguished by the insertion of 240 bp into the spacer between H1 and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.1.225
更新日期:1989-01-11 00:00:00
abstract::Transgenic mice suitable for the in vivo assay of suspected mutagens at the chromosome level have been constructed by stable integration of a lambda phage shuttle vector. The shuttle vector, which contains a beta-galactosidase (beta-gal) target gene, can be rescued from genomic DNA with in vitro packaging extracts. Mu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.10.3007
更新日期:1990-05-25 00:00:00
abstract::Gene II protein is the only phage-encoded protein required for the double-stranded DNA replication of the distantly related filamentous phages IKe and Ff (M13, fd and f1). Complementation studies have demonstrated that, despite a significant degree of homology between the nucleotide sequences of the gene II's of IKe a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.12.5067
更新日期:1986-06-25 00:00:00
abstract::The KEGG RPAIR database is a collection of biochemical structure transformation patterns, called RDM patterns, and chemical structure alignments of substrate-product pairs (reactant pairs) in all known enzyme-catalyzed reactions taken from the Enzyme Nomenclature and the KEGG PATHWAY database. Here, we present PathPre...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq318
更新日期:2010-07-01 00:00:00