Abstract:
:Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous with the mRNA sequence coding for amino acids 42-45 and used it as a primer to selectively transcribe an interferon cDNA template. The sequence of the newly synthesised DNA predicted the sequence of amino acids 48-109 in the interferon polypeptide. By repeating this process with one more primer, we have determined the complete amino acid sequence of mature human fibroblast interferon, a polypeptide of 166 amino acids.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Houghton M,Easton MA,Stewart AG,Smith JC,Doel SM,Catlin GH,Lewis HM,Patel TP,Emtage JS,Carey NH,Porter AGdoi
10.1093/nar/8.13.2885subject
Has Abstractpub_date
1980-07-11 00:00:00pages
2885-94issue
13eissn
0305-1048issn
1362-4962journal_volume
8pub_type
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journal_title:Nucleic acids research
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