Abstract:
:A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction fragment length polymorphisms to provide genetic markers on chromosome 21. Nine independently isolated clones used as probes identified a total of 11 new RFLPs. Four of the DNA probes recovered from the library have been mapped unequivocally to chromosome 21 using a panel of somatic cell hybrid lines. A fifth probe detected an RFLP on chromosome 21 as well as sequences on other chromosomes. This set of RFLPs may now form the basis for construction of a genetic linkage map of human chromosome 21.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Watkins PC,Tanzi RE,Gibbons KT,Tricoli JV,Landes G,Eddy R,Shows TB,Gusella JFdoi
10.1093/nar/13.17.6075subject
Has Abstractpub_date
1985-09-11 00:00:00pages
6075-88issue
17eissn
0305-1048issn
1362-4962journal_volume
13pub_type
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