Abstract:
:Bacterial retrons consist of a reverse transcriptase (RT) and a contiguous non-coding RNA (ncRNA) gene. One third of annotated retrons carry additional open reading frames (ORFs), the contribution and significance of which in retron biology remains to be determined. In this study we developed a computational pipeline for the systematic prediction of genes specifically associated with retron RTs based on a previously reported large dataset representative of the diversity of prokaryotic RTs. We found that retrons generally comprise a tripartite system composed of the ncRNA, the RT and an additional protein or RT-fused domain with diverse enzymatic functions. These retron systems are highly modular, and their components have coevolved to different extents. Based on the additional module, we classified retrons into 13 types, some of which include additional variants. Our findings provide a basis for future studies on the biological function of retrons and for expanding their biotechnological applications.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Mestre MR,González-Delgado A,Gutiérrez-Rus LI,Martínez-Abarca F,Toro Ndoi
10.1093/nar/gkaa1149subject
Has Abstractpub_date
2020-12-16 00:00:00pages
12632-12647issue
22eissn
0305-1048issn
1362-4962pii
6020195journal_volume
48pub_type
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