Abstract：
:A simple, reproducible affinity chromatography method has been adapted for separation of high molecular weight supercoiled circular molecules from mammalian cells. Electron microscopic analysis of EB viral DNA obtained by this method, from the non-producer Burkitt's lymphoma line Raji, revealed monomer-sized viral molecules only. In contrast, the EB viral episomes from recently established human producer lines BL-8 and LY91 were very heterogeneous in size, some being considerably smaller and others much larger than the monomeric DNA. The former are probably related to defective viral species in the B-cell population, but the origin of the latter are as yet unclear. All cell lines contained both monomers and concatemers of mitochondrial DNA; among the latter, molecules apparently greater than 100 kb were observed in the population.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kinchington D,Griffin BEdoi
10.1093/nar/15.24.10345subject
Has Abstractpub_date
1987-12-23 00:00:00pages
10345-54issue
24eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract：:The non-essential MGS1 gene of Saccharomyces cerevisiae is highly conserved in eukaryotes and encodes an enzyme containing both DNA-dependent ATPase and DNA annealing activities. MGS1 appears to function in post-replicational repair processes that contribute to genome stability. In this study, we identified MGS1 as a ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki900
更新日期：2005-10-26 00:00:00
abstract：:Since 1992, FlyBase (flybase.org) has been an essential online resource for the Drosophila research community. Concentrating on the most extensively studied species, Drosophila melanogaster, FlyBase includes information on genes (molecular and genetic), transgenic constructs, phenotypes, genetic and physical interacti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1016
更新日期：2017-01-04 00:00:00
abstract：:An attempt has been made to correlate differential scanning calorimetry melting profiles of 5S rRNAs from lupin seeds (L.s.) and wheat germ (W.g.) with their structure. It is suggested that the observed differences in thermal unfolding are due to differences in RNA nucleotide sequence and as a consequence in higher or...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.2.685
更新日期：1988-01-25 00:00:00
abstract：:Recognition of DNA by proteins depends on DNA sequence and structure. Often unanswered is whether the structure of naked DNA persists in a protein-DNA complex, or whether protein binding changes DNA shape. While X-ray structures of protein-DNA complexes are numerous, the structure of naked cognate DNA is seldom availa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky033
更新日期：2018-03-16 00:00:00
abstract：:Ribonuclease MRP is an endonuclease, related to RNase P, which functions in eukaryotic pre-rRNA processing. In Saccharomyces cerevisiae, RNase MRP comprises an RNA subunit and ten proteins. To improve our understanding of subunit roles and enzyme architecture, we have examined protein-protein and protein-RNA interacti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm553
更新日期：2007-01-01 00:00:00
abstract：:The Central glycolytic genes Repressor (CggR) from Bacillus subtilis belongs to the SorC family of transcription factors that control major carbohydrate metabolic pathways. Recent studies have shown that CggR binds as a tetramer to its tandem operator DNA sequences and that the inducer metabolite, fructose 1,6-bisphos...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq334
更新日期：2010-09-01 00:00:00
abstract：:The Gene Context Tool (GeConT) allows users to visualize the genomic context of a gene or a group of genes and their orthologous relationships within fully sequenced bacterial genomes. The new version of the server incorporates information from the COG, Pfam and KEGG databases, allowing users to have an integrated gra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn330
更新日期：2008-07-01 00:00:00
abstract：:The crystal structure of d(CATGGGCCCATG)(2) shows unique stacking patterns of a stable B<-->A-DNA intermediate. We evaluated intrinsic base stacking energies in this crystal structure using an ab initio quantum mechanical method. We found that all crystal base pair steps have stacking energies close to their values in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.24.4893
更新日期：2000-12-15 00:00:00
abstract：:The promoter of the human BCR gene, regulating the transcription of the chimeric BCR/ABL mRNA in leukemia, has been isolated and characterized. A region of 1.1 kb immediately 5' to the transcription start site was analyzed in detail by sequencing, DNase 1 footprinting, gel retardation and functional studies. These exp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.23.7119
更新日期：1990-12-11 00:00:00
abstract：:Sensory photoreceptors have enabled non-invasive and spatiotemporal control of numerous biological processes. Photoreceptor engineering has expanded the repertoire beyond natural receptors, but to date no generally applicable strategy exists towards constructing light-regulated protein actuators of arbitrary function....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw930
更新日期：2016-11-16 00:00:00
abstract：:Protein classification by machine learning algorithms is now widely used in structural and functional annotation of proteins. The Protein Classification Benchmark collection (http://hydra.icgeb.trieste.it/benchmark) was created in order to provide standard datasets on which the performance of machine learning methods ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl812
更新日期：2007-01-01 00:00:00
abstract：:We have previously mapped a repression domain from the active transcriptional repressor E4BP4 to a 65 amino acid segment near the C-terminus of the polypeptide. Here we show that the E4BP4 repression domain interacts specifically with the TBP binding repressor protein Dr1. Mutants that affect the ability of E4BP4 to b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.18.3607
更新日期：1996-09-15 00:00:00
abstract：:A differential effect is found of various bivalent cations (Ba2+, Ca2+, Mg2+, Cd2+, Co2+, Mn2+, Ni2+, Zn2+ and Hg2+) on stability of intermolecular Py-Pu-Pu triplex with different sequence of base triads. Ca2+, Mg2+, Cd2+, Co2+, Mn2+, Ni2+ and Zn2+ do stabilize the d(C)n d(G)n d(G)n triplex whereas Ba2+ and Hg2+ do no...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.3.585
更新日期：1993-02-11 00:00:00
abstract：:The titration viscometric investigation of the multi-mode interaction of netropsin (Nt) with (A.T) clusters of NaDNA12 and NH4DNA10 has been extended to different temperatures. The position of two boundaries on the r-scale (r= [Nt]bound/[DNA-P]) with increasing temperature steadily (rI/II) or more abruptly (rO/I) shif...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.10.2335
更新日期：1981-05-25 00:00:00
abstract：:Polyploidy, the presence of multiple sets of chromosomes that are similar but not identical, complicates both chromosome walking and assembly of sequence-ready contigs for many plant taxa including a large number of economically-significant crops. Traditional 'dot-blot hybridization' or PCR-based assays for identifyin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.7.e23
更新日期：2000-04-01 00:00:00
abstract：:Atomistic molecular dynamics (MD) simulations generate a wealth of information related to the dynamics of proteins. If properly analyzed, this information can lead to new insights regarding protein function and assist wet-lab experiments. Aiming to identify interactions between individual amino acid residues and the r...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky381
更新日期：2018-07-02 00:00:00
abstract：:We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.8.1897
更新日期：1992-04-25 00:00:00
abstract：:Interferons (IFNs) are a family of multifunctional proteins involved in immune activation, regulation of cell growth and antiviral response. They exert their functions by induction of several IFN-stimulated genes, including IFN regulatory factors (IRFs), a family of transcriptional regulators. One of these factors, IR...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.21.4219
更新日期：2000-11-01 00:00:00
abstract：:Peptide nucleic acids (PNAs) can bind to homopurine/homopyrimidine sequences of double-stranded DNA targets in a sequence-specific manner and form [PNA]2/DNA triplexes with single-stranded DNA D-loop structures at the PNA binding sites. These D-loop structures have been found to have a capacity to initiate transcripti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.13.2806
更新日期：1999-07-01 00:00:00
abstract：:The cellular response to DNA double-strand breaks (DSBs) is initiated by the Mre11-Rad50-Xrs2 (MRX) complex that has structural and catalytic functions. MRX association at DSBs is counteracted by Rif2, which is known to interact with Rap1 that binds telomeric DNA through two tandem Myb-like domains. Whether and how Ra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz1203
更新日期：2020-03-18 00:00:00
abstract：:CAG repeats occur predominantly in the coding regions of human genes, which suggests their functional importance. In some genes, these sequences can undergo pathogenic expansions leading to neurodegenerative polyglutamine (poly-Q) diseases. The mutant transcripts containing expanded CAG repeats possibly contribute to ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq700
更新日期：2010-12-01 00:00:00
abstract：:In eukaryotes, exposure to stress conditions causes a shift from cap-dependent to cap-independent translation. In trypanosomatids, environmental switches are the driving force of a developmental program of gene expression, but it is yet unclear how their translation machinery copes with their constantly changing envir...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr555
更新日期：2011-10-01 00:00:00
abstract：:Conventional chemotherapy remains the primary treatment option for triple-negative breast cancer (TNBC). However, the current chemotherapeutic drugs have limited effects on TNBC, and often lead to serious side effects as well as drug resistance. Thus, more effective therapeutic options are sorely needed. As c-MYC onco...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz835
更新日期：2019-11-18 00:00:00
abstract：:Accumulating evidence has demonstrated that transcriptional regulation is affected by DNA methylation. Understanding the perturbation of DNA methylation-mediated regulation between transcriptional factors (TFs) and targets is crucial for human diseases. However, the global landscape of DNA methylation-mediated transcr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa041
更新日期：2020-03-18 00:00:00
abstract：:Bacteria occupy a diverse set of environmental niches with differing oxygen availability. Anaerobic environments such as mammalian digestive tracts and industrial reactors harbor an abundance of both obligate and facultative anaerobes, many of which play significant roles in human health and biomanufacturing. Studying...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw580
更新日期：2016-09-30 00:00:00
abstract：:HIV-1 reverse transcriptase (RT) is a primary target for anti-AIDS drugs. Structures of HIV-1 RT, usually determined at approximately 2.5-3.0 A resolution, are important for understanding enzyme function and mechanisms of drug resistance in addition to being helpful in the design of RT inhibitors. Despite hundreds of ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn464
更新日期：2008-09-01 00:00:00
abstract：:A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme cleavage. The amin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.21.4923
更新日期：1993-10-25 00:00:00
abstract：:Upon infection of Escherichia coli by bacteriophage Qβ, the virus-encoded β-subunit recruits host translation elongation factors EF-Tu and EF-Ts and ribosomal protein S1 to form the Qβ replicase holoenzyme complex, which is responsible for amplifying the Qβ (+)-RNA genome. Here, we use X-ray crystallography, NMR spect...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1212
更新日期：2015-12-15 00:00:00
abstract：:Backscattering interferometry (BSI) has been used to successfully monitor molecular interactions without labeling and with high sensitivity. These properties suggest that this approach might be useful for detecting biomarkers of infection. In this report, we identify interactions and characteristics of nucleic acid pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt165
更新日期：2013-05-01 00:00:00
abstract：:Analysis of intergenic sequences for purposes such as the investigation of transcriptional signals or the identification of small RNA genes is frequently complicated by traditional biological database structures. Genome data is commonly treated as chromosome-length sequence records, detailed by gene calls demarcating ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg013
更新日期：2003-01-01 00:00:00
