Abstract：
:We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes and half of the cloned DNA sequences analysed are unique or low copy. Approximately half of these sequences when used as probes detect sequences on wheat chromosome 4A. The generation and analysis of the chromosome library is described in detail and the prospects of using flow-sorted plant chromosomes discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Wang ML,Leitch AR,Schwarzacher T,Heslop-Harrison JS,Moore Gdoi
10.1093/nar/20.8.1897keywords:
subject
Has Abstractpub_date
1992-04-25 00:00:00pages
1897-901issue
8eissn
0305-1048issn
1362-4962journal_volume
20pub_type
杂志文章abstract：:The methyltransferase, M.EcoKI, recognizes the DNA sequence 5'-AACNNNNNNGTGC-3' and methylates adenine at the underlined positions. DNA methylation has been shown by crystallography to occur via a base flipping mechanism and is believed to be a general mechanism for all methyltransferases. If no structure is available...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh531
更新日期：2004-04-23 00:00:00
abstract：:The structure of eukaryotic chromatin has been investigated by isolating and analyzing the "accessible" DNA fraction of rat liver chromatin. This DNA fraction has been isolated by titrating the chromatin with the protese-resistant D isomer of polylysine to bind the "accessible" DNA sites. After removal of chromosomal ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.5.667
更新日期：1975-05-01 00:00:00
abstract：:Treatment of the ciliated protozoan Tetrahymena thermophila with 8-methoxypsoralen combined with long wavelength ultraviolet irradiation (UVA, lambda approximately 360 nm) resulted in a dose dependent equal inhibition of the synthesis of rRNA, 5.8S, 5S and tRNA. Similar results were obtained with 3-carbethoxy-8-methox...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.3.921
更新日期：1987-02-11 00:00:00
abstract：:Error-free lesion bypass and error-prone lesion bypass are important cellular responses to DNA damage during replication, both of which require a DNA polymerase (Pol). To identify lesion bypass DNA polymerases, we have purified human Polkappa encoded by the DINB1 gene and examined its response to damaged DNA templates...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.21.4138
更新日期：2000-11-01 00:00:00
abstract：:Sgs1 is a member of the RecQ family of DNA helicases, which have been implicated in genomic stability, cancer and ageing. Srs2 is another DNA helicase that shares several phenotypic features with Sgs1 and double sgs1srs2 mutants have a severe synthetic growth phenotype. This suggests that there may be functional overl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.5.1103
更新日期：2002-03-01 00:00:00
abstract：:DNA synthesis is a fundamental requirement for cell proliferation and DNA repair, but no single method can identify the location, direction and speed of replication forks with high resolution. Mammalian cells have the ability to incorporate thymidine analogs along with the natural A, T, G and C bases during DNA synthe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa517
更新日期：2020-09-04 00:00:00
abstract：:Plant orthologs of the yeast sucrose non-fermenting (Snf1) kinase and mammalian AMP-activated protein kinase (AMPK) represent an emerging class of important regulators of metabolic and stress signalling. The catalytic alpha-subunits of plant Snf1-related kinases (SnRKs) interact in the yeast two-hybrid system with dif...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.17.3685
更新日期：2001-09-01 00:00:00
abstract：:Alternative RNA splicing is an essential process to yield proteomic diversity in eukaryotic cells, and aberrant splicing is often associated with numerous human diseases and cancers. We recently described serine/arginine-rich splicing factor 3 (SRSF3 or SRp20) being a proto-oncogene. However, the SRSF3-regulated splic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1500
更新日期：2016-02-29 00:00:00
abstract：:Transcription of transfer RNA genes by RNA polymerase III (Pol III) is controlled by general factors, TFIIIB and TFIIIC, and a negative regulator, Maf1. Here we report the interplay between TFIIIC and Maf1 in controlling Pol III activity upon the physiological switch of yeast from fermentation to respiration. TFIIIC d...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky656
更新日期：2018-10-12 00:00:00
abstract：:Chromatin organization and epigenetic markers influence splicing, though the magnitudes of these effects and the mechanisms are largely unknown. Here, we demonstrate that linker histone H1.5 influences mRNA splicing. We observed that linker histone H1.5 binds DNA over splice sites of short exons in human lung fibrobla...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz338
更新日期：2019-07-09 00:00:00
abstract：:IStrons are chimeric genetic elements composed of a group I intron associated with an insertion sequence (IS). The group I intron is a catalytic RNA providing the IStron with self-splicing ability, which renders IStron insertions harmless to the host genome. The IS element is a DNA transposon conferring mobility, and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku939
更新日期：2014-11-10 00:00:00
abstract：:Restriction endonucleases are highly specific in recognizing the particular DNA sequence they act on. However, their activity is affected by sequence context, enzyme concentration and buffer composition. Changes in these factors may lead to either ineffective cleavage at the cognate restriction site or relaxed specifi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt257
更新日期：2013-06-01 00:00:00
abstract：:We describe a versatile genetic system for rapid analysis of mammalian gene function. In this, loss of reporter activity in a novel embryonic stem (ES) cell line enables rapid identification of targeting to the ubiquitously expressed Rosa26 locus. Subsequent regulation of gene activity is governed by a dual regulatory...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni146
更新日期：2005-10-12 00:00:00
abstract：:5' Terminal cap structures of hnRNA have been characterized and the extent of capping determined as a function of embryonic development. Sea urchin embryo hnRNA contains only the type-1 cap, m7GpppNmpNp, with the type-2 cap, which has a 2'-0-methylated subpenultimate nucleotide, being associated only with stable small...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.6.2307
更新日期：1979-01-01 00:00:00
abstract：:We have analyzed the DNaseI sensitivity of chromatin from the rat albumin and alpha-fetoprotein genes in the fetal liver (which synthesizes albumin and alpha-fetoprotein), adult liver (which synthesizes albumin), fetal yolk sac (which synthesizes alpha-fetoprotein), and adult kidney (which synthesizes neither). Active...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.1.115
更新日期：1985-01-11 00:00:00
abstract：:The RESID Database is a comprehensive collection of annotations and structures for protein post-translational modifications including N-terminal, C-terminal and peptide chain cross-link modifications. The RESID Database includes systematic and frequently observed alternate names, Chemical Abstracts Service registry nu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.1.199
更新日期：2001-01-01 00:00:00
abstract：:Chromatin immunoprecipitation (ChIP) has ushered in a new era of scientific discovery by allowing new insights into DNA-protein interactions. ChIP is used to quantify enriched genomic regions using qPCR, and more recently is combined with next generation sequencing (ChIP-seq) to obtain a genome wide profile of protein...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky813
更新日期：2019-01-08 00:00:00
abstract：:For the first time mosaic nucleic acids composed of 50% RNA and 50% DNA can be obtained as transcripts with T7 RNA polymerase. Two NTPs could be replaced simultaneously in a transcription reaction. This means more than 40 deoxynucleotides were inserted in one transcript. Previously, a maximum of two deoxynucleotides c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.11.1845
更新日期：1995-06-11 00:00:00
abstract：:I-motif DNAs have been widely employed as robust modulating components to construct reconfigurable DNA nanodevices that function well in acidic cellular environments. However, they generally display poor interactivity with fluorescent ligands under these complex conditions, illustrating a major difficulty in utilizing...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa020
更新日期：2020-02-28 00:00:00
abstract：:IntAct is an open source database and software suite for modeling, storing and analyzing molecular interaction data. The data available in the database originates entirely from published literature and is manually annotated by expert biologists to a high level of detail, including experimental methods, conditions and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl958
更新日期：2007-01-01 00:00:00
abstract：:When the function of origins of replication in yeast was compromised by placing ARS sequences downstream of strong promoters, ARS activity might have been affected either by transcription or by an altered chromatin configuration induced by the construct. To distinguish between these possibilities, derivatives of the y...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.19.3904
更新日期：1994-09-25 00:00:00
abstract：:The primary rRNA transcript contains a large external transcribed spacer (ETS) approximately 4,000 nucleotides in length. We have used subcloned DNA probes derived from the 5' end of the ETS in conjunction with Northern blot analysis of murine nuclear RNA to examine processing of this region. In agreement with the res...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.18.7187
更新日期：1984-09-25 00:00:00
abstract：:DNA swivel (nicking-rejoining) enzyme activity has been studied in various cell fractions of a human lymphoid cell line. Swivel activity is found only in chromatin and in a nuclear membrane fraction containing DNA and possessing endogenous DNA synthesizing activity. Twenty percent of the total swivel activity and less...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.1.223
更新日期：1977-01-01 00:00:00
abstract：:Fragments of SP6 DNA generated by cleavage with Hpa II or Taq I were cloned into the Cla I site of pBR322 and the recombinant plasmids were screened for the presence of SP6 promoter activity by transcription in vitro with purified SP6 RNA polymerase. Three plasmids having promoter activity and small inserts of SP6 DNA...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.8.3521
更新日期：1986-04-25 00:00:00
abstract：:This study unveils Mycobacterium smegmatis DinB2 as the founder of a clade of Y-family DNA polymerase that is naturally adept at incorporating ribonucleotides by virtue of a leucine in lieu of a canonical aromatic steric gate. DinB2 efficiently scavenges limiting dNTP and rNTP substrates in the presence of manganese. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku752
更新日期：2014-01-01 00:00:00
abstract：:Light is a major external factor in regulating seed germination. Photoreceptor phytochrome B (PHYB) plays a predominant role in promoting seed germination in the initial phase after imbibition, partially by repressing phytochrome-interacting factor1 (PIF1). However, the mechanism underlying the PHYB-PIF1-mediated tran...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx283
更新日期：2017-07-07 00:00:00
abstract：:The Escherichia coli fmu gene product has recently been determined to be the 16S rRNA m(5)C 967 methyltransferase. As such, Fmu represents the first protein identified as an S -adenosyl-L-methionine (AdoMet)- dependent RNA m(5)C methyltransferase whose amino acid sequence is known. Using the amino acid sequence of Fmu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.15.3138
更新日期：1999-08-01 00:00:00
abstract：:MS²PIP is a data-driven tool that accurately predicts peak intensities for a given peptide's fragmentation mass spectrum. Since the release of the MS²PIP web server in 2015, we have brought significant updates to both the tool and the web server. In addition to the original models for CID and HCD fragmentation, we hav...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz299
更新日期：2019-07-02 00:00:00
abstract：:Diazo-based precursors of photolabile groups have been used extensively for modifying nucleic acids, with the intention of toggling biological processes with light. These processes include transcription, translation and RNA interference. In these cases, the photolabile groups have been typically depicted as modifying ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp415
更新日期：2009-07-01 00:00:00
abstract：:The Trypanosoma brucei exoribonuclease, TbDSS-1, has been implicated in multiple aspects of mitochondrial RNA metabolism. Here, we investigate the role of TbDSS-1 in RNA processing and surveillance by analyzing 12S rRNA processing intermediates in TbDSS-1 RNAi cells. RNA fragments corresponding to leader sequence upst...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm690
更新日期：2008-01-01 00:00:00
