当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > Transcription through the yeast origin of replication ARS1 ends at the ABFI binding site and affects extrachromosomal maintenance of minichromosomes.

Transcription through the yeast origin of replication ARS1 ends at the ABFI binding site and affects extrachromosomal maintenance of minichromosomes.

Abstract：

:When the function of origins of replication in yeast was compromised by placing ARS sequences downstream of strong promoters, ARS activity might have been affected either by transcription or by an altered chromatin configuration induced by the construct. To distinguish between these possibilities, derivatives of the yeast TRP1ARS1 minichromosome were constructed that contained either the DED1 or the PET56 promoter firing against ARS1 (DEDARS and PETARS constructs). PETARS constructs transformed yeast at high frequencies and were maintained as minichromosomes consistent with efficient ARS1 function, but DEDARS constructs transformed at low frequencies and had to be rescued as minichromosomes by insertion of a second ARS (H4-ARS). Chromatin analysis revealed that the ARS1 regions in PETARS and H4-DEDARS constructs were indistinguishable from the ARS1 region of the host TRP1ARS1 circle showing a nuclease sensitive region flanked by a nucleosome. However, RNA-analysis in the ARS region showed high and low levels of transcripts in H4-DEDARS and PETARS, respectively. Transcription elongated through the A, B1, and B2 elements and ended in B3, the binding site for ABFI. We conclude that transcription through ARS1 and not an altered chromatin structure affected ARS activity in these constructs.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Tanaka S,Halter D,Livingstone-Zatchej M,Reszel B,Thoma F

doi

10.1093/nar/22.19.3904

subject

Has Abstract

pub_date

1994-09-25 00:00:00

pages

3904-10

issue

19

eissn

0305-1048

issn

1362-4962

journal_volume

22

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • Characterisation of a genomic clone covering the structural mouse MyoD1 gene and its promoter region.

    abstract：:We have isolated the mouse MyoD1 gene flanked by its promoter region by screening a genomic library with synthetic oligonucleotides. The structural gene is interrupted by two G + C rich introns. Transfection of the cloned gene inserted into an expression vector converts fibroblasts to myoblasts. Sequence analysis of a...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/19.23.6433

    authors： Zingg JM,Alva GP,Jost JP

    更新日期：1991-12-11 00:00:00

  • NF-kappa B-independent activation of beta-interferon expression in mouse F9 embryonal carcinoma cells.

    abstract：:We have examined the behaviour of the beta-interferon promoter in mouse F9 embryonal carcinoma cells. In undifferentiated cells, the beta-interferon promoter is not responsive to dsRNA or to Sendai virus. In cells stimulated to differentiate into parietal endoderm by treatment with retinoic acid, the beta-interferon p...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/22.21.4489

    authors： Ellis MJ,Goodbourn S

    更新日期：1994-10-25 00:00:00

  • The rat poly pyrimidine tract binding protein (PTB) interacts with a single-stranded DNA motif in a liver-specific enhancer.

    abstract：:We characterized and purified a protein from rat liver which specifically binds to a DNA motif present in a liver-specific enhancer of the rat tyrosine aminotransferase (TAT) gene, when offered as single-stranded DNA. Binding is highly sequence-specific and coincides with a region known to be essential for function of...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.6.1243

    authors： Jansen-Dürr P,Boshart M,Lupp B,Bosserhoff A,Frank RW,Schütz G

    更新日期：1992-03-25 00:00:00

  • T7 promoter contacts essential for promoter activity in vivo.

    abstract：:T7 RNA polymerase promoters consist of a highly conserved 23 base-pair sequence that spans the site of the initiation of transcription (+1) and extends from -17 to +6. To determine the bases within the T7 consensus promoter that are essential for promoter function a library of mutant T7 promoters was constructed, and ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.10.2517

    authors： Ikeda RA,Ligman CM,Warshamana S

    更新日期：1992-05-25 00:00:00

  • A pentatricopeptide repeat protein acts as a site-specificity factor at multiple RNA editing sites with unrelated cis-acting elements in plastids.

    abstract：:In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks164

    authors： Okuda K,Shikanai T

    更新日期：2012-06-01 00:00:00

  • Modification of the aminopyridine unit of 2'-deoxyaminopyridinyl-pseudocytidine allowing triplex formation at CG interruptions in homopurine sequences.

    abstract：:The antigene strategy based on site-specific recognition of duplex DNA by triplex DNA formation has been exploited in a wide range of biological activities. However, specific triplex formation is mostly restricted to homo-purine strands within the target duplex DNA, due to the destabilizing effect of CG and TA inversi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky704

    authors： Wang L,Taniguchi Y,Okamura H,Sasaki S

    更新日期：2018-09-28 00:00:00

  • A minor class of 5S rRNA genes in Saccharomyces cerevisiae X2180-1B, one member of which lies adjacent to a Ty transposable element.

    abstract：:In Saccharomyces cerevisiae the majority of the genes for 5S rRNA lie within a 9kb rDNA sequence that is present as 100-200 tandemly-repeated copies on Chromosome XII. Following our observations that about 10% of yeast 5S rRNA exists as minor variant sequences, we screened a collection of yeast DNA fragments cloned in...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/12.10.4083

    authors： Piper PW,Lockheart A,Patel N

    更新日期：1984-05-25 00:00:00

  • MicroRNA-mediated up-regulation of an alternatively polyadenylated variant of the mouse cytoplasmic {beta}-actin gene.

    abstract：:Actin is a major cytoskeletal protein in eukaryotes. Recent studies suggest more diverse functional roles for this protein. Actin mRNA is known to be localized to neuronal synapses and undergoes rapid deadenylation during early developmental stages. However, its 3'-untranslated region (UTR) is not characterized and th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkn624

    authors： Ghosh T,Soni K,Scaria V,Halimani M,Bhattacharjee C,Pillai B

    更新日期：2008-11-01 00:00:00

  • Diels-Alder cycloadditions in water for the straightforward preparation of peptide-oligonucleotide conjugates.

    abstract：:The Diels-Alder reaction between diene-modified oligonucleotides and maleimide-derivatized peptides afforded peptide-oligonucleotide conjugates with high purity and yield. Synthesis of the reagents was easily accomplished by on-column derivatization of the corresponding peptides and oligonucleotides. The cycloaddition...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gnj020

    authors： Marchán V,Ortega S,Pulido D,Pedroso E,Grandas A

    更新日期：2006-02-14 00:00:00

  • Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification.

    abstract：:In prokaryotes and archaea transfer ribonucleic acid (tRNA) stability as well as cellular UV protection relies on the post-transcriptional modification of uracil at position 8 (U8) of tRNAs by the 4-thiouridine synthetase ThiI. Here, we report three crystal structures of ThiI from Thermotoga maritima in complex with a...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku249

    authors： Neumann P,Lakomek K,Naumann PT,Erwin WM,Lauhon CT,Ficner R

    更新日期：2014-06-01 00:00:00

  • Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer.

    abstract：:As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.12.3233

    authors： Yoshimura K,Rosenfeld MA,Nakamura H,Scherer EM,Pavirani A,Lecocq JP,Crystal RG

    更新日期：1992-06-25 00:00:00

  • REV1 and polymerase ζ facilitate homologous recombination repair.

    abstract：:REV1 and DNA Polymerase ζ (REV3 and REV7) play important roles in translesion DNA synthesis (TLS) in which DNA replication bypasses blocking lesions. REV1 and Polζ have also been implicated in promoting repair of DNA double-stranded breaks (DSBs). However, the mechanism by which these two TLS polymerases increase tole...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkr769

    authors： Sharma S,Hicks JK,Chute CL,Brennan JR,Ahn JY,Glover TW,Canman CE

    更新日期：2012-01-01 00:00:00

  • BRUCE: a program for the detection of transfer-messenger RNA genes in nucleotide sequences.

    abstract：:A computer program, BRUCE, was developed for the identification of transfer-messenger RNA (tmRNA) genes. The program employs heuristic algorithms to search for a tRNA(Ala)-like secondary structure surrounding a short sequence encoding the tag peptide. In the 57 completely sequenced bacterial genomes where tmRNA genes ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkf459

    authors： Laslett D,Canback B,Andersson S

    更新日期：2002-08-01 00:00:00

  • Solid phase synthesis of oligoribonucleotides using the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) group for the protection of the 2'-hydroxy functions and the H-phosphonate approach.

    abstract：:The solid phase synthesis of oligoribonucleotides using the H-phosphonate approach and the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) and dimethoxytrityl (DMTr) groups, respectively, for the protection of the 2'- and 5'-hydroxy functions is described. The use of a new reagent, tris-(1,1,1,3,3,3-hexaf...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/17.10.3689

    authors： Sakatsume O,Ohtsuki M,Takaku H,Reese CB

    更新日期：1989-05-25 00:00:00

  • Effect of 2',3'-dideoxythymidine-5'-triphosphate on HeLa cell in vitro DNA synthesis: evidence that DNA polymerase alpha is the only polymerase required for cellular DNA replication.

    abstract：:We have studied the effects of the nucleotide analogue, 2',3'-dideoxythymidine-5'-triphosphate (ddTTP) on replicative DNA synthesis in HeLa cell lysates. As previously demonstrated (1), such lysates carry out extensive DNA synthesis in vitro, at rates and in a fashion similar to in vivo DNA replication. We report here...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/5.6.1933

    authors： Waqar MA,Evans MJ,Huberman JA

    更新日期：1978-06-01 00:00:00

  • Localization of DNA methyltransferase in the chromatin of Friend erythroleukemia cells.

    abstract：:Chromatin fragments released from intact Friend erythroleukemia cell nuclei during limited incubation with micrococcal nuclease, DNase II or DNase I were analyzed to determine the distribution of DNA methyltransferase in chromatin. The enzyme was released in a free form when internucleosomal DNA was digested with micr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/9.20.5359

    authors： Creusot F,Christman JK

    更新日期：1981-10-24 00:00:00

  • Structure and function of the Toscana virus cap-snatching endonuclease.

    abstract：:Toscana virus (TOSV) is an arthropod-borne human pathogen responsible for seasonal outbreaks of fever and meningoencephalitis in the Mediterranean basin. TOSV is a segmented negative-strand RNA virus (sNSV) that belongs to the genus phlebovirus (family Phenuiviridae, order Bunyavirales), encompassing other important h...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkz838

    authors： Jones R,Lessoued S,Meier K,Devignot S,Barata-García S,Mate M,Bragagnolo G,Weber F,Rosenthal M,Reguera J

    更新日期：2019-11-18 00:00:00

  • Rational design of point mutation-selective antisense DNA targeted to codon 12 of Ha-ras mRNA in human cells.

    abstract：:Antisense oligodeoxynucleotides targeted to Ha-ras mRNA have been designed to discriminate between the codon 12-mutated oncogene and the normal proto-oncogene. An in vitro assay using two different sources of RNase H (rabbit reticulocyte lysates and nuclear extract from HeLa cells) was used to characterize oligonucleo...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/23.17.3411

    authors： Duroux I,Godard G,Boidot-Forget M,Schwab G,Hélène C,Saison-Behmoaras T

    更新日期：1995-09-11 00:00:00

  • BACH1 recruits NANOG and histone H3 lysine 4 methyltransferase MLL/SET1 complexes to regulate enhancer-promoter activity and maintains pluripotency.

    abstract：:Maintenance of stem-cell identity requires proper regulation of enhancer activity. Both transcription factors OCT4/SOX2/NANOG and histone methyltransferase complexes MLL/SET1 were shown to regulate enhancer activity, but how they are regulated in embryonic stem cells (ESCs) remains further studies. Here, we report a t...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkab034

    authors： Niu C,Wang S,Guo J,Wei X,Jia M,Chen Z,Gong W,Qin Y,Wang X,Zhi X,Lu M,Chen S,Gu M,Zhang J,Han JJ,Lan F,Meng D

    更新日期：2021-01-27 00:00:00

  • Significant expansion of the REST/NRSF cistrome in human versus mouse embryonic stem cells: potential implications for neural development.

    abstract：:Recent studies have employed cross-species comparisons of transcription factor binding, reporting significant regulatory network 'rewiring' between species. Here, we address how a transcriptional repressor targets and regulates neural genes differentially between human and mouse embryonic stem cells (ESCs). We find th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv514

    authors： Rockowitz S,Zheng D

    更新日期：2015-07-13 00:00:00

  • Partial purification and characterization of two cytoplasmic DNA polymerases from ungerminated wheat.

    abstract：:Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/2.11.2077

    authors： Castroviejo M,Tarragó-Litvak L,Litvak S

    更新日期：1975-11-01 00:00:00

  • An ATF/CREB binding motif is required for aberrant constitutive expression of the MHC class II DR alpha promoter and activation by SV40 T-antigen.

    abstract：:Constitutive expression of major histocompatibility complex class II (MHC II) antigens normally occurs in B-lymphocytes and antigen presenting cells of the monocyte/macrophage lineage. However, many malignant tumours and transformed cells express these proteins aberrantly. We demonstrate here that the MHC II DR alpha ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.18.4881

    authors： Cox PM,Goding CR

    更新日期：1992-09-25 00:00:00

  • Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking.

    abstract：:Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light microscopic particle observation is usually limited to two-dimensional projections of short trajectory segments, we report here imag...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku1194

    authors： Spille JH,Kaminski TP,Scherer K,Rinne JS,Heckel A,Kubitscheck U

    更新日期：2015-01-01 00:00:00

  • iPcc: a novel feature extraction method for accurate disease class discovery and prediction.

    abstract：:Gene expression profiling has gradually become a routine procedure for disease diagnosis and classification. In the past decade, many computational methods have been proposed, resulting in great improvements on various levels, including feature selection and algorithms for classification and clustering. In this study,...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt343

    authors： Ren X,Wang Y,Zhang XS,Jin Q

    更新日期：2013-08-01 00:00:00

  • ChIP-Enrich: gene set enrichment testing for ChIP-seq data.

    abstract：:Gene set enrichment testing can enhance the biological interpretation of ChIP-seq data. Here, we develop a method, ChIP-Enrich, for this analysis which empirically adjusts for gene locus length (the length of the gene body and its surrounding non-coding sequence). Adjustment for gene locus length is necessary because ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku463

    authors： Welch RP,Lee C,Imbriano PM,Patil S,Weymouth TE,Smith RA,Scott LJ,Sartor MA

    更新日期：2014-07-01 00:00:00

  • Some KpnI family members are associated with the Alu family in the human genome.

    abstract：:The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5' terminal) was found to be common to both members. The Alu family sequence was found in the 5' flanking regions. The KpnI ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/11.19.6837

    authors： Miyake T,Migita K,Sakaki Y

    更新日期：1983-10-11 00:00:00

  • Different types of interaction between PCNA and PIP boxes contribute to distinct cellular functions of Y-family DNA polymerases.

    abstract：:Translesion DNA synthesis (TLS) by the Y-family DNA polymerases Polη, Polι and Polκ, mediated via interaction with proliferating cell nuclear antigen (PCNA), is a crucial pathway that protects human cells against DNA damage. We report that Polη has three PCNA-interacting protein (PIP) boxes (PIP1, 2, 3) that contribut...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv712

    authors： Masuda Y,Kanao R,Kaji K,Ohmori H,Hanaoka F,Masutani C

    更新日期：2015-09-18 00:00:00

  • Reversion of a transcriptionally defective MHC class II-negative human B-cell mutant.

    abstract：:RJ2.2.5, a mutant derived from the human B-lymphoma cell, Raji, is unable to express the MHC class II genes because of a recessive transcriptional defect attributed to the lack of an activator function. We report the isolation of a RJ2.2.5 revertant, namely AR, in which the expression of the mRNAs encoded by these gen...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/21.3.381

    authors： Ombra MN,Perfetto C,Autiero M,Anzisi AM,Pasquinelli R,Maffei A,Del Pozzo G,Guardiola J

    更新日期：1993-02-11 00:00:00

  • On the mechanism of tRNA methylase-tRNA recognition.

    abstract：:In order to further elucidate the mechanism of tRNA methylase-tRNA intreaction the methylation of some individual tRNAs separately and by pairs was performed. In conditions of tRNA excess the methylation rates of positionally analogous nucleotides in tRNA molecules are not summed up when two substrates are simultaneou...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/3.8.2079

    authors： Gambaryan AS,Venkstern TV,Bayev AA

    更新日期：1976-08-01 00:00:00

  • Late induction of human DNA ligase I after UV-C irradiation.

    abstract：:We have studied the regulation of DNA ligase I gene expression in UV-C irradiated human primary fibroblasts. An increase of approximately 6-fold both in DNA ligase I messenger and activity levels was observed 24 h after UV treatment, when nucleotide excision repair (NER) is no longer operating. DNA ligase I induction ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/23.6.962

    authors： Montecucco A,Savini E,Biamonti G,Stefanini M,Focher F,Ciarrocchi G

    更新日期：1995-03-25 00:00:00