Abstract:
:The Diels-Alder reaction between diene-modified oligonucleotides and maleimide-derivatized peptides afforded peptide-oligonucleotide conjugates with high purity and yield. Synthesis of the reagents was easily accomplished by on-column derivatization of the corresponding peptides and oligonucleotides. The cycloaddition reaction was carried out in mild conditions, in aqueous solution at 37 degrees C. The speed of the reaction was found to vary depending on the size of the reagents, but it can be completed in 8-10 h by reacting the diene-oligonucleotide with a small excess of maleimide-peptide.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Marchán V,Ortega S,Pulido D,Pedroso E,Grandas Adoi
10.1093/nar/gnj020keywords:
subject
Has Abstractpub_date
2006-02-14 00:00:00pages
e24issue
3eissn
0305-1048issn
1362-4962pii
34/3/e24journal_volume
34pub_type
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