Abstract:
:Because of their high nucleotide sequence homology, the specific detection of mRNA transcripts of individual members of a gene family presents certain problems. Here we apply and defend the single nucleotide primer extension (SNuPE) as a simple, specific, and highly quantitative assay for this purpose. The method distinguishes regions of the brain sodium channel gene family that vary by as little as a single nucleotide. The technique has been modified to include an intersample normalizer, and adaptation of the SNuPE assay to other gene families of interest to neuroscientists should be easy to achieve.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Lombardo AJ,Brown GBdoi
10.1093/nar/24.23.4812subject
Has Abstractpub_date
1996-12-01 00:00:00pages
4812-6issue
23eissn
0305-1048issn
1362-4962pii
6g0329journal_volume
24pub_type
杂志文章abstract::Cyclophilins are a family of proteins that share a common, highly conserved sequence motif. Cyclophilins bind transiently to other proteins and facilitate their folding. One member of the family, hCypH, is part of the human spliceosomal [U4/U6.U5] tri-snRNP complex; it associates specifically and stably with the U4/U6...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg660
更新日期:2003-08-15 00:00:00
abstract::TORQUE is a tool for cross-species querying of protein-protein interaction networks. It aims to answer the following question: given a set of proteins constituting a known complex or a pathway in one species, can a similar complex or pathway be found in the protein network of another species? To this end, Torque seeks...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp474
更新日期:2009-07-01 00:00:00
abstract::Combinatorial interactions of sequence-specific trans-acting factors with localized genomic cis-element clusters are the principal mechanism for regulating tissue-specific and developmental gene expression. With the emergence of expanding numbers of genome-wide expression analyses, the identification of the cis-elemen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki486
更新日期:2005-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx745
更新日期:2017-10-13 00:00:00
abstract::The Est3 subunit of yeast telomerase, which adopts a predicted OB-fold, is essential for telomere replication. To assess the possible contributions that Est3 might make to enzyme catalysis, we compared telomerase activity from wild type and est3-Delta strains of Saccharomyces castellii, which revealed that loss of the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp1173
更新日期:2010-04-01 00:00:00
abstract::Transcription of the human adenovirus 5 genome in transformed rat embryo cells (DFK3) was investigated using two different approaches. Preferential digestion of transcribed viral sequences by DNase I was analysed using kinetics of renaturation of 32P-labeled Ad5 HpaI restriction fragments in the presence of material w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.1.1
更新日期:1978-01-01 00:00:00
abstract::Brome mosaic virus (BMV) is a tripartite genome, positive-sense RNA virus of plants. Previously it was demonstrated that local hybridization between BMV RNAs (RNA-RNA heteroduplex formation) efficiently promotes non-homologous RNA recombination. In addition, studies on the role of the BMV polymerase in RNA recombinati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.8.1714
更新日期:2000-04-15 00:00:00
abstract::Extensive research has characterized distinct exogenous RNAi pathways interfering in gene expression during vegetative growth of the unicellular model ciliate Paramecium. However, role of RNAi in endogenous transcriptome regulation, and environmental adaptation is unknown. Here, we describe the first genome-wide profi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz553
更新日期:2019-09-05 00:00:00
abstract::The high-throughput sequencing of nuclease-protected mRNA fragments bound to ribosomes, a technique known as ribosome profiling, quantifies the relative frequencies with which different regions of transcripts are translated. This technique has revealed novel translation initiation sites with unprecedented scope and ha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv200
更新日期:2015-04-20 00:00:00
abstract::A series of plasmids were constructed in which a promoter was introduced into a lac-based operon fusion vector. A perfectly symmetrical oligonucleotide of 22-bp corresponding to an idealized binding site for cAMP receptor protein (CRP) of E. coli was chemically synthesized. The synthetic CRP site was placed between th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.15.7315
更新日期:1988-08-11 00:00:00
abstract::The ciliate Tetrahymena thermophila is an important eukaryotic model organism that has been used in pioneering studies of general phenomena, such as ribozymes, telomeres, chromatin structure and genome reorganization. Recent work has shown that Tetrahymena has many classes of small RNA molecules expressed during veget...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr792
更新日期:2012-02-01 00:00:00
abstract::When designed to cleave a target RNA in trans, the hammerhead ribozyme contains two antisense flanks which form helix I and helix III by pairing with the complementary target RNA. The sequences forming helix II are contained on the ribozyme strand and represent a major structural component of the hammerhead structure....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.19.3951
更新日期:1994-09-25 00:00:00
abstract::Short repetitive DNA sequences are believed to be one of the primordial genetic elements that served as a source of complex large DNA found in the genome of modern organisms. However, the mechanism of its expansion (increase in repeat number) during the course of evolution is unclear. We demonstrate that the DNA polym...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.20.3999
更新日期:2000-10-15 00:00:00
abstract::Calf thymus DNA polymerase alpha (pol alpha) and bacteriophage T4 DNA polymerase (pol T4) were exploited as model enzymes to investigate the molecular mechanism of inhibitory action of N2-(p-n-butylphenyl)dGTP (BuPdGTP) and 2-(p-n-butyl-anilino)dATP (BuAdATP) on the BuPdNTP-susceptible alpha polymerase family. Kinetic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.7.1627
更新日期:1991-04-11 00:00:00
abstract::In this paper we focus on the detection of differentially expressed genes according to changes in hybridization signals using statistical tests. These tests were applied to 14 208 zebrafish cDNA clones that were immobilized on a nylon support and hybridized with radioactively labeled target mRNA from wild-type and lit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.23.e117
更新日期:2001-12-01 00:00:00
abstract::The HIV RT and Protease Sequence Database is an online relational database that catalogs evolutionary and drug-related human immunodeficiency virus (HIV) reverse transcriptase (RT) and protease sequence variation (http://hivdb.stanford.edu). The database contains a compilation of nearly all published HIV RT and protea...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.1.346
更新日期:2000-01-01 00:00:00
abstract::Oligoamines (spermidine, dipropylenetriamine and propylenediamine) were covalently attached to acridine via a hexamethylene linker. These oligoamine-acridine conjugates were efficiently bound to gap sites in substrate DNA, and promoted the DNA hydrolysis by a homogeneous Ce(IV)/ethylenediamine-N,N,N',N'-tetraacetate (...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg634
更新日期:2003-08-01 00:00:00
abstract::It is well established that gene regulation can be achieved through activator and repressor proteins that bind to DNA and switch particular genes on or off, and that complex metabolic networks determine the levels of transcription of a given gene at a given time. Using three complementary computational techniques to s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw815
更新日期:2016-11-02 00:00:00
abstract::Cationic aromatic tricyclic compounds including triphenylmethane dyes, phenazines, phenoxazines, acridines, phenothiazines, phenanthridinium compounds, anthracenes and xanthene dyes, which amplify cell killing in phleomycin-treated Escherichia coli B cells also modified phleomycin-induced breakdown of DNA to acid-solu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.23.9083
更新日期:1984-12-11 00:00:00
abstract::A single base change in 16S rRNA (C726 to G) has previously been shown to have a dramatic effect on protein synthesis in E. coli (1). This paper more specifically details the effects of the mutation on mRNA binding and translation-termination. The in vitro technique of toeprinting (2) was used to demonstrate that 30S ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.18.5381
更新日期:1990-09-25 00:00:00
abstract::The repetitive fraction of the genome of the eukaryotic slime mould Physarum polycephalum is dominated by the Tp1 family of highly repetitive retrotransposon-like sequences. Tp1 elements consist of two terminal direct repeats of 277bp which flank an internal domain of 8.3kb. They are the major sequence component in th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.2.279
更新日期:1991-01-25 00:00:00
abstract::Several authors, including ourselves, have reported the existence of chromatosomes with DNA size larger than 166 bp in bird erythrocyte chromatin. It was tempting to correlate this increased DNA size with the presence of histone H5. In order to substantiate this hypothesis, we performed a micrococcal nuclease digestio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.15.3671
更新日期:1981-08-11 00:00:00
abstract::Muscle creatine kinase (MCK) is a major enzyme of cellular energy metabolism that is expressed upon differentiation of myoblasts into myotubes. Previously we cloned and sequenced the entire rabbit enzyme cDNA which was used as a probe in these studies to obtain a genomic clone from a rabbit library. The transcription ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.11.3027
更新日期:1991-06-11 00:00:00
abstract::Circular dichroism in the 300-360 nm region and fluorescence induced by intercaltating binding of ethidum bromide to both DNA and RNA components were studied in isolated HeLa nucleoli. Both DNA and RNA compoents contribute to the induced dichroic elliticity. Digestion of nucleoli by RNase or DNase shows that most of t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.8.1857
更新日期:1976-08-01 00:00:00
abstract::We have used 2-aminopurine (2AP) as a fluorescent probe in the template strand of a 13/20mer primer/template (D) to detect deoxynucleoside triphosphates (N)-dependent conformational changes exhibited by RB69 DNA polymerase (ED) complexes. The rates and amplitudes of fluorescence quenching depend hyperbolically on the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm587
更新日期:2007-01-01 00:00:00
abstract::Approximately 2% of the Dictyostelium discoideum genome consists of multiple copies of a retrotransposable element termed DRE (Dictyostelium Repetitive Element). These elements have always been found integrated in a position and orientation-specific manner 50 +/- 4 nucleotides upstream of the coding region of tRNA gen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.23.6247
更新日期:1992-12-11 00:00:00
abstract::During DNA replication, the presence of 8-oxoguanine (8-oxoG) lesions in the template strand cause the high-fidelity (HiFi) DNA polymerase (Pol) to stall. An early response to 8-oxoG lesions involves 'on-the-fly' translesion synthesis (TLS), in which a specialized TLS Pol is recruited and replaces the stalled HiFi Pol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa803
更新日期:2020-11-04 00:00:00
abstract::Highly toxic DNA double-strand breaks (DSBs) readily trigger the DNA damage response (DDR) in cells, which delays cell cycle progression to ensure proper DSB repair. In Saccharomyces cerevisiae, mitotic S phase (20-30 min) is lengthened upon DNA damage. During meiosis, Spo11-induced DSB onset and repair lasts up to 5 ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa587
更新日期:2020-09-04 00:00:00
abstract::RNA interference represents a potent intervention for cancer treatment but requires a robust delivery agent for transporting gene-modulating molecules, such as small interfering RNAs (siRNAs). Although numerous molecular approaches for siRNA delivery are adequate in vitro, delivery to therapeutic targets in vivo is li...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz900
更新日期:2019-12-02 00:00:00
abstract::Prokaryotic genomic DNA is generally negatively supercoiled in vivo. Many regulatory processes, including the initiation of transcription, are known to depend on the superhelical state of the DNA substrate. The stresses induced within DNA by negative superhelicity can destabilize the DNA duplex at specific sites. Vari...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj007
更新日期:2006-01-01 00:00:00