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Studies of the transcription of viral genome in adenovirus 5 transformed cells.

Abstract：

:Transcription of the human adenovirus 5 genome in transformed rat embryo cells (DFK3) was investigated using two different approaches. Preferential digestion of transcribed viral sequences by DNase I was analysed using kinetics of renaturation of 32P-labeled Ad5 HpaI restriction fragments in the presence of material which was stable after nuclease treatment. The second approach was the hybridization of 32P-labeled nuclear RNA from transformed cells with Ad5 restriction fragments which were attached to a nitrocellulose filter. These two methods gave similar results. It was found that not all integrated regions of the Ad5 genome are active in transformed cells. 2,5 copies of the HpaI-E fragment of Ad5 DNA were found in transformed DFK3 cell line. Nuclear RNA from these cells hybridized to HpaI-E fragment of Ad5 DNA, but only about half of sequences of the integrated HpaI-E fragment was sensitive to DNase I digestion.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Frolova EI,Zalmanzon ES,Lukanidin EM,Georgiev GP

doi

10.1093/nar/5.1.1

subject

Has Abstract

pub_date

1978-01-01 00:00:00

pages

1-11

issue

1

eissn

0305-1048

issn

1362-4962

journal_volume

5

pub_type

杂志文章

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