Abstract：
:Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence and other promoter mutations located in this region.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Munson LM,Mandecki W,Caruthers MH,Reznikoff WSdoi
10.1093/nar/12.9.4011subject
Has Abstractpub_date
1984-05-11 00:00:00pages
4011-7issue
9eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract：:Protein-RNA interactions play essential roles in a number of regulatory mechanisms for gene expression such as RNA splicing, transport, translation and post-transcriptional control. As the number of available protein-RNA complex 3D structures has increased, it is now possible to statistically examine protein-RNA inter...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl819
更新日期：2006-01-01 00:00:00
abstract：:PrimPol is a primase-polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been established. Here, we report that the proposed zinc finger domain ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku214
更新日期：2014-05-01 00:00:00
abstract：:We present a general method for isolating molecular markers specific to any region of a chromosome using existing mapping populations. Two pools of DNA from individuals homozygous for opposing alleles for a targeted chromosomal interval, defined by two or more linked RFLP markers, are constructed from members of an ex...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.23.6553
更新日期：1991-12-11 00:00:00
abstract：:The Nucleolar Proteome Database (NOPdb) archives data on >700 proteins that were identified by multiple mass spectrometry (MS) analyses from highly purified preparations of human nucleoli, the most prominent nuclear organelle. Each protein entry is annotated with information about its corresponding gene, its domain st...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkj004
更新日期：2006-01-01 00:00:00
abstract：:The identification of genes with specific patterns of change (e.g. down-regulated and methylated) as phenotype drivers or samples with similar profiles for a given gene set as drivers of clinical outcome, requires the integration of several genomic data types for which an 'integrate by intersection' (IBI) approach is ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1503
更新日期：2016-04-20 00:00:00
abstract：:Understanding the molecular mechanisms that influence transposable element target site preferences is a fundamental challenge in functional and evolutionary genomics. Large-scale transposon insertion projects provide excellent material to study target site preferences in the absence of confounding effects of post-inse...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn563
更新日期：2008-11-01 00:00:00
abstract：:The rRNA WWW Server at URL http://rrna.uia.ac.be/ now provides a database of 496 large subunit ribosomal RNA sequences. All these sequences are aligned, incorporate secondary structure information, and can be obtained in a number of formats. Other information about the sequences, such as literature references, accessi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.1.183
更新日期：1998-01-01 00:00:00
abstract：:An improved algorithm was elaborated to analyse tRNA interaction with aminoacyl-tRNA synthetase based on analysis of tRNA sequences. The fundamental element defining the interaction between the tRNA and the synthetase is not a single nucleotide but a nucleotide combination named a tile which comprises of a given nucle...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.8.1941
更新日期：1993-04-25 00:00:00
abstract：:The rates of acid-catalyzed hydrolysis of a hypermodified nucleoside, wyosine and its 5'-monophosphate were determined at various pH, temperature and buffer concentrations. The results show that despite distinct differences in structure and the glycosyl bond stability, the hydrolysis of wyosine proceeds via cleavage o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.7.2443
更新日期：1985-04-11 00:00:00
abstract：:U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, h...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp050
更新日期：2009-04-01 00:00:00
abstract：:Antigenic variation of the pilus is an essential component of Neisseria gonorrhoeae pathogenesis. Unidirectional recombination of silent pilin DNA into an expressed pilin gene allows for substantial sequence variation of this highly immunogenic surface structure. While the RecA protein is required for pilin gene recom...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.7.1362
更新日期：1997-04-01 00:00:00
abstract：:Hydroxylapatite chromatographies of mitochondrial and total enzymes from a rho+ yeast, or from the related rho degrees mitochondrial DNA-less mutant, show the occurrence in the mitochondrial enzyme of one Phe-, one Met-, one Leu-tRNA synthetase peak which elutes distinctly from the cytoplasmic counterpart and charges ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.5.1151
更新日期：1976-05-01 00:00:00
abstract：:Aberration within the p53 tumor suppressor gene is the most frequently identified genetic damage in human cancer. Regulatory functions proposed for the p53 protein include modulation of the cell cycle, cellular differentiation, signal transduction, and gene expression. Additionally, the p53 gene product may guard the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.3.713
更新日期：1993-02-11 00:00:00
abstract：:SRS (Sequence Retrieval System) is a widely used keyword search engine for querying biological databases. BLAST2 is the most widely used tool to query databases by sequence similarity search. These tools allow users to retrieve sequences by shared keyword or by shared similarity, with many public web servers available...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg535
更新日期：2003-07-01 00:00:00
abstract：:G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. T...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.7.1613
更新日期：1998-04-01 00:00:00
abstract：:Programmed chromosome breakage occurs at 50-200 specific sites in the genome of Tetrahymena thermo-phila during somatic nuclear (macronuclear) differentiation. Previous studies have identified a 15 bp sequence, the Cbs (for chromosome breakage sequence), that is necessary and sufficient to specify these sites. In this...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.4.895
更新日期：2000-02-15 00:00:00
abstract：:The CATH database of protein domain structures (http://www.biochem.ucl.ac.uk/bsm/cath_new) currently contains 34 287 domain structures classified into 1383 superfamilies and 3285 sequence families. Each structural family is expanded with domain sequence relatives recruited from GenBank using a variety of efficient seq...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg062
更新日期：2003-01-01 00:00:00
abstract：:We report thermodynamic values for binding of the guanosine nucleophile to the ribozyme derived from the Anabaena group I intron, and find that they are similar to those measured previously for the structurally distinct Tetrahymena ribozyme. The free energy of binding guanosine 5'-monophosphate (pG) at 30 degrees C is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.19.3722
更新日期：1996-10-01 00:00:00
abstract：:The sequence of 436 nucleotides around the region coding for 5.8S RNA in the Sciara coprophila rDNA transcription unit (1) has been determined. Regions coding for 5.8S and 2S RNAs have been identified; they are 80 - 90% homologous to the corresponding Drosophila sequences and are separated by a 22 nucleotide long spac...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.16.3565
更新日期：1980-08-25 00:00:00
abstract：:The three dimensional crystal structure of T5 5'-3' exonuclease was compared with that of two other members of the 5'-3' exonuclease family: T4 ribonuclease H and the N-terminal domain of Thermus aquaticus DNA polymerase I. Though these structures were largely similar, some regions of these enzymes show evidence of si...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.21.4224
更新日期：1997-11-01 00:00:00
abstract：:Genotoxic stress induces alternative splicing of the oncogene MDM2 generating MDM2-ALT1, an isoform attributed with tumorigenic properties. However, the mechanisms underlying this event remain unclear. Here we explore MDM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2 splicing in respon...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv223
更新日期：2015-04-30 00:00:00
abstract：:A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.17.6075
更新日期：1985-09-11 00:00:00
abstract：:Epigenetic regulation of gene expression is tightly controlled by the dynamic modification of histones by chemical groups, the diversity of which has largely expanded over the past decade with the discovery of lysine acylations, catalyzed from acyl-coenzymes A. We investigated the dynamics of lysine acetylation and cr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa163
更新日期：2020-05-07 00:00:00
abstract：:Multiplexed tandem PCR (MT-PCR) is a process for highly multiplexed gene expression profiling. In the first step, multiple primer pairs are added to the RNA to be analysed together with reverse transcriptase and Taq DNA polymerase. Following reverse transcription, the multiplexed amplicons are simultaneously amplified...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni182
更新日期：2005-11-24 00:00:00
abstract：:An effective tool for the global analysis of both DNA methylation status and protein-chromatin interactions is a microarray constructed with sequences containing regulatory elements. One type of array suited for this purpose takes advantage of the strong association between CpG Islands (CGIs) and gene regulatory regio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki582
更新日期：2005-05-23 00:00:00
abstract：:SynergyFinder (https://synergyfinder.fimm.fi) is a stand-alone web-application for interactive analysis and visualization of drug combination screening data. Since its first release in 2017, SynergyFinder has become a widely used web-tool both for the discovery of novel synergistic drug combinations in pre-clinical mo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa216
更新日期：2020-07-02 00:00:00
abstract：:Arguably, the most bizarre mitochondrial DNA (mtDNA) is that of the euglenozoan eukaryote Diplonema papillatum. The genome consists of numerous small circular chromosomes none of which appears to encode a complete gene. For instance, the cox1 coding sequence is spread out over nine different chromosomes in non-overlap...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq883
更新日期：2011-02-01 00:00:00
abstract：:Molecular dynamics (MD) simulations and time resolved fluorescence (TRF) spectroscopy were combined to quantitatively describe the conformational landscape of the DNA primary binding sequence (PBS) of the HIV-1 genome, a short hairpin targeted by retroviral nucleocapsid proteins implicated in the viral reverse transcr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw077
更新日期：2016-04-20 00:00:00
abstract：:In the most extensive examination to date of the relationship between the pausing of reverse transcrip-tase (RT) and RNA secondary structures, pause events were found to be correlated to inverted repeats both ahead of, and behind the catalytic site in vitro. In addition pausing events were strongly associated with pol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.14.3433
更新日期：1998-07-15 00:00:00
abstract：:PIR-International is an association of macromolecular sequence data collection centers dedicated to fostering international cooperation as an essential element in the development of scientific databases. A major objective of PIR-International is to continue the development of the Protein Sequence Database as an essent...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
