Abstract:
:Using cyanoethyldiisopropylamino phosphoramidite chemistry, four oligonucleotides constituting a part of the sequence of the initiator t-RNA from B. subtilis were synthesized. For the protection of the exocyclic amino functions of bases, phenoxyacetyl group was used for adenine and guanine, and acetyl group was preferred for cytosine. With these labile groups, final deprotection of the oligonucleotides can be performed in milder conditions, allowing the incorporation of 5,6-dihydrouridine in a 35-mer constituting the 5'-end of the t-RNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Chaix C,Duplaa AM,Molko D,Téoule Rdoi
10.1093/nar/17.18.7381subject
Has Abstractpub_date
1989-09-25 00:00:00pages
7381-93issue
18eissn
0305-1048issn
1362-4962journal_volume
17pub_type
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