Solid phase synthesis of the 5'-half of the initiator t-RNA from B. subtilis.

Abstract:

:Using cyanoethyldiisopropylamino phosphoramidite chemistry, four oligonucleotides constituting a part of the sequence of the initiator t-RNA from B. subtilis were synthesized. For the protection of the exocyclic amino functions of bases, phenoxyacetyl group was used for adenine and guanine, and acetyl group was preferred for cytosine. With these labile groups, final deprotection of the oligonucleotides can be performed in milder conditions, allowing the incorporation of 5,6-dihydrouridine in a 35-mer constituting the 5'-end of the t-RNA.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Chaix C,Duplaa AM,Molko D,Téoule R

doi

10.1093/nar/17.18.7381

subject

Has Abstract

pub_date

1989-09-25 00:00:00

pages

7381-93

issue

18

eissn

0305-1048

issn

1362-4962

journal_volume

17

pub_type

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