Abstract：
:Successful use and reliability of microarray technology is highly dependent on several factors, including surface chemistry parameters and accessibility of cDNA targets to the DNA probes fixed onto the surface. Here, we show that functionalisation of glass slides with homemade dendrimers allow production of more sensitive and reliable DNA microarrays. The dendrimers are nanometric structures of size-controlled diameter with aldehyde function at their periphery. Covalent attachment of these spherical reactive chemical structures on amino-silanised glass slides generates a reactive approximately 100 A layer onto which amino-modified DNA probes are covalently bound. This new grafting chemistry leads to the formation of uniform and homogenous spots. More over, probe concentration before spotting could be reduced from 0.2 to 0.02 mg/ml with PCR products and from 20 to 5 micro M with 70mer oligonucleotides without affecting signal intensities after hybridisation with Cy3- and Cy5-labelled targets. More interestingly, while the binding capacity of captured probes on dendrimer-activated glass surface (named dendrislides) is roughly similar to other functionalised glass slides from commercial sources, detection sensitivity was 2-fold higher than with other available DNA microarrays. This detection limit was estimated to 0.1 pM of cDNA targets. Altogether, these features make dendrimer-activated slides ideal for manufacturing cost-effective DNA arrays applicable for gene expression and detection of mutations.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Le Berre V,Trévisiol E,Dagkessamanskaia A,Sokol S,Caminade AM,Majoral JP,Meunier B,François Jdoi
10.1093/nar/gng088keywords:
subject
Has Abstractpub_date
2003-08-15 00:00:00pages
e88issue
16eissn
0305-1048issn
1362-4962journal_volume
31pub_type
杂志文章abstract：:RegulonDB is a DataBase that integrates biological knowledge of the mechanisms that regulate the transcription initiation in Escherichia coli , as well as knowledge on the organization of the genes and regulatory signals into operons in the chromosome. The operon is the basic structure used in RegulonDB to describe th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.1.55
更新日期：1998-01-01 00:00:00
abstract：:The accumulation of somatic mitochondrial DNA (mtDNA) mutations contributes to the pathogenesis of human disease. Currently, mitochondrial mutations are largely considered results of inaccurate processing of its heavily damaged genome. However, mainly from a lack of methods to monitor mtDNA mutations with sufficient s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw716
更新日期：2016-10-14 00:00:00
abstract：:Circular DNA can arise from all parts of eukaryotic chromosomes. In yeast, circular ribosomal DNA (rDNA) accumulates dramatically as cells age, however little is known about the accumulation of other chromosome-derived circles or the contribution of such circles to genetic variation in aged cells. We profiled circular...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa545
更新日期：2020-08-20 00:00:00
abstract：:ADAR enzymes, adenosine deaminases that act on RNA, form a family of RNA editing enzymes that convert adenosine to inosine within RNA that is completely or largely double-stranded. Site-selective A-->I editing has been detected at specific sites within a few structured pre-mRNAs of metazoans. We have analyzed the edit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg681
更新日期：2003-08-15 00:00:00
abstract：:ProPortal (http://proportal.mit.edu/) is a database containing genomic, metagenomic, transcriptomic and field data for the marine cyanobacterium Prochlorococcus. Our goal is to provide a source of cross-referenced data across multiple scales of biological organization--from the genome to the ecosystem--embracing the f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1022
更新日期：2012-01-01 00:00:00
abstract：:Recruitment of RAD18 to stalled replication forks facilitates monoubiquitination of PCNA during S-phase, promoting translesion synthesis at sites of UV irradiation-induced DNA damage. In this study, we show that RAD18 is also recruited to ionizing radiation (IR)-induced sites of DNA double-strand breaks (DSBs) forming...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp082
更新日期：2009-04-01 00:00:00
abstract：:Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and the consequent a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku105
更新日期：2014-04-01 00:00:00
abstract：:The reactivities of the two cysteine thiol groups of calf thymus F3 histone were investigated using 5,5'-dithiobis-[2- nitrobenzoic acid], (DTNB). In isolated histone, both thiol groups were available for reaction. However, analysis of reaction profiles of native deoxyribonucleohistone, (DNH), in various solvent condi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.2.203
更新日期：1974-02-01 00:00:00
abstract：:'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base substitutions in the center of the primers. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.16.3745
更新日期：1993-08-11 00:00:00
abstract：:The essential splicing factor Prp24 contains four RNA Recognition Motif (RRM) domains, and functions to anneal U6 and U4 RNAs during spliceosome assembly. Here, we report the structure and characterization of the C-terminal RRM4. This domain adopts a novel non-canonical RRM fold with two additional flanking α-helices ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr455
更新日期：2011-09-01 00:00:00
abstract：:We have prepared cDNA libraries for amoebae and plasmodia of the acellular slime mould, Physarum polycephalum. Differential screening was used to isolate cell-type-specific cDNA clones (in bacteriophage M13) and both libraries yielded approximately 5% of such sequences. The amoebal- and plasmodial-specific clones were...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.3.933
更新日期：1987-02-11 00:00:00
abstract：:Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1034
更新日期：2016-02-18 00:00:00
abstract：:We have developed an improved method for photofootprinting in vivo which utilizes the thermostable DNA polymerase from T. aquaticus (Taq) in a primer extension assay. UV light is used to introduce photoproducts into the genomic DNA of intact yeast cells. The photoproducts are then detected and mapped at the nucleotide...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.1.171
更新日期：1989-01-11 00:00:00
abstract：:A fraction of the ribosomal 28S genes in some insects are interrupted at specific sites by insertion elements R1 and R2 (also called Type I and II). These elements contain long open-reading frames with homology to reverse transcriptase. We have identified in the silkmoth, Bombyx mori, copies of these elements which ha...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.22.10561
更新日期：1988-11-25 00:00:00
abstract：:DNA double-strand breaks (DSBs) and their repair can cause extensive epigenetic changes. As a result, DSBs have been proposed to promote transcriptional and, ultimately, physiological dysfunction via both cell-intrinsic and cell-non-autonomous pathways. Studying the consequences of DSBs in higher organisms has, howeve...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1482
更新日期：2016-04-20 00:00:00
abstract：:We report the molecular cloning and characterization of a cDNA derived from a zebrafish gene (ZF-21) related to the mouse homeobox containing gene Hox2.1. Interesting information about the differential conservation of various domains was gained from comparisons between the putative protein sequences from ZF-21 (275 am...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.19.9097
更新日期：1988-10-11 00:00:00
abstract：:Chemically modified siRNAs are expected to have resistance toward nuclease degradation and good thermal stability in duplex formation for in vivo applications. We have recently found that 2'-OMe-4'-thioRNA, a hybrid chemical modification based on 2'-OMeRNA and 4'-thioRNA, has high hybridization affinity for complement...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks204
更新日期：2012-07-01 00:00:00
abstract：:Differential scanning microcalorimetry (DSC), UV absorption and circular dichroism (CD) have been used to study structure and stability of linear (lin), open circular (oc), supercoiled (cd) and relaxed circular duplex (rd) DNA and calf thymus (CT) DNA. Investigations were made in low salt buffer and in the presence of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.24.11737
更新日期：1988-12-23 00:00:00
abstract：:Recent advances in DNA-sequencing technology have made it possible to obtain large datasets of small RNA sequences. Here we demonstrate that not all non-perfectly matched small RNA sequences are simple technological sequencing errors, but many hold valuable biological information. Analysis of three small RNA datasets ...
journal_title：Nucleic acids research
pub_type： 杂志文章,meta分析
doi：10.1093/nar/gkp093
更新日期：2009-05-01 00:00:00
abstract：:Although mobility of the phylogenetically widespread group I introns appears to be mechanistically similar, the phage T4 intron-encoded endonucleases that promote mobility of the td and sunY introns are different from their eukaryotic counterparts. Most notably, they cleave at a distance from the intron insertion site...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.13.3763
更新日期：1990-07-11 00:00:00
abstract：:Peptide nucleic acids (PNAs) can bind to homopurine/homopyrimidine sequences of double-stranded DNA targets in a sequence-specific manner and form [PNA]2/DNA triplexes with single-stranded DNA D-loop structures at the PNA binding sites. These D-loop structures have been found to have a capacity to initiate transcripti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.13.2806
更新日期：1999-07-01 00:00:00
abstract：:O-GLYCBASE is a revised database of information on glycoproteins and their O-linked glycosylation sites. Entries are compiled and revised from the literature, and from the sequence databases. Entries include information about species, sequence, glycosylation sites and glycan type and is fully cross-referenced. Compare...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.1.387
更新日期：1998-01-01 00:00:00
abstract：:Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.22.9255
更新日期：1987-11-25 00:00:00
abstract：:In order to study the effects of DNA structure on cellular processes such as transcription, we have made a series of plasmids that locate several different kinds of DNA structure (stiff, flexible or curved) near the sites of cleavage by commonly-used restriction enzymes. One can use these plasmids to place any DNA reg...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.22.9447
更新日期：1989-11-25 00:00:00
abstract：:The transcription factor Sox5 has previously been shown in chicken to be expressed in early neural crest cells and neural crest-derived peripheral glia. Here, we show in mouse that Sox5 expression also continues after neural crest specification in the melanocyte lineage. Despite its continued expression, Sox5 has litt...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn527
更新日期：2008-10-01 00:00:00
abstract：:The rDNA cluster in Saccharomyces cerevisiae is located 450 kb from the left end and 610 kb from the right end of chromosome XII and consists of approximately 150 tandemly repeated copies of a 9.1 kb rDNA unit. To explore the biological significance of this specific chromosomal context, chromosome XII was split at bot...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl293
更新日期：2006-05-31 00:00:00
abstract：:The effect of [Na+] on the stability and conformation of penicillium chrysogenum mycophage dsRNA (PCMdsRNA) was investigated using CD and UV optical techniques. Thermal melting profiles reveal prominent fine structure attributed to at least four regions of structural dissimilarity. A constant increased thermal stabili...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.6.759
更新日期：1975-06-01 00:00:00
abstract：:PAX6 is a transcription factor that plays a major role in ocular morphogenesis. PAX6 is expressed in the eye, central nervous system and pancreas. Two alternative promoters, P0 and P1, which are differentially regulated during development, drive PAX6 transcription. We identified a 57 bp cis-regulatory element in exon ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.19.4070
更新日期：2001-10-01 00:00:00
abstract：:The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) is Europe's primary resource for nucleotide sequence information. With the growing volume and diversity of public sequencing data comes the need for increased sophistication in data organisation, presentation and search services so as to maximise its disc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1129
更新日期：2015-01-01 00:00:00
abstract：:RNA editing in protozoan parasites is a mitochondrial RNA processing reaction in which exclusively uridylate residues are inserted into, and less frequently deleted from, pre-mRNAs. Molecules central to the process are so-called guide RNAs (gRNAs) which function as templates in the reaction. For a detailed molecular u...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.12.2311
更新日期：1997-06-15 00:00:00
