Primary structure, developmentally regulated expression and potential duplication of the zebrafish homeobox gene ZF-21.

abstract：:Chlorella virus PBCV-1 DNA ligase seals nicked DNA substrates consisting of a 5'-phosphate-terminated strand and a 3'-hydroxyl-terminated strand annealed to a bridging DNA template strand. The enzyme discriminates at the DNA binding step between substrates containing a 5'-phosphate versus a 5'-hydroxyl at the nick. Mu...

journal_title：Nucleic acids research

authors： Sriskanda V,Shuman S

更新日期：1998-01-15 00:00:00

abstract：:Recognizing the methylation status of specific DNA sequences is central to the function of many systems in eukaryotes and prokaryotes. Restriction-modification systems have to distinguish between 'self' and 'non-self' DNA and depend on the inability of restriction endonucleases to cleave their DNA substrates when the ...

journal_title：Nucleic acids research

authors： Rice MR,Blumenthal RM

更新日期：2000-08-15 00:00:00

abstract：:The NCBI Taxonomy database (http://www.ncbi.nlm.nih.gov/taxonomy) is the standard nomenclature and classification repository for the International Nucleotide Sequence Database Collaboration (INSDC), comprising the GenBank, ENA (EMBL) and DDBJ databases. It includes organism names and taxonomic lineages for each of the...

journal_title：Nucleic acids research

authors： Federhen S

更新日期：2012-01-01 00:00:00

abstract：:Antisense oligodeoxynucleotides targeted to Ha-ras mRNA have been designed to discriminate between the codon 12-mutated oncogene and the normal proto-oncogene. An in vitro assay using two different sources of RNase H (rabbit reticulocyte lysates and nuclear extract from HeLa cells) was used to characterize oligonucleo...

journal_title：Nucleic acids research

authors： Duroux I,Godard G,Boidot-Forget M,Schwab G,Hélène C,Saison-Behmoaras T

更新日期：1995-09-11 00:00:00

abstract：:A series of plasmids were constructed in which a promoter was introduced into a lac-based operon fusion vector. A perfectly symmetrical oligonucleotide of 22-bp corresponding to an idealized binding site for cAMP receptor protein (CRP) of E. coli was chemically synthesized. The synthetic CRP site was placed between th...

journal_title：Nucleic acids research

authors： Morita T,Shigesada K,Kimizuka F,Aiba H

更新日期：1988-08-11 00:00:00

abstract：:Complex carbohydrates of plants are the main food sources of animals and microbes, and serve as promising renewable feedstock for biofuel and biomaterial production. Carbohydrate active enzymes (CAZymes) are the most important enzymes for complex carbohydrate metabolism. With an increasing number of plant and plant-as...

journal_title：Nucleic acids research

authors： Zhang H,Yohe T,Huang L,Entwistle S,Wu P,Yang Z,Busk PK,Xu Y,Yin Y

更新日期：2018-07-02 00:00:00

abstract：:The active form of Escherichia coli DNA polymerase V responsible for damage-induced mutagenesis is a multiprotein complex (UmuD'(2)C-RecA-ATP), called pol V Mut. Optimal activity of pol V Mut in vitro is observed on an SSB-coated single-stranded circular DNA template in the presence of the β/γ complex and a transactiv...

journal_title：Nucleic acids research

authors： Vaisman A,Kuban W,McDonald JP,Karata K,Yang W,Goodman MF,Woodgate R

更新日期：2012-07-01 00:00:00

abstract：:To examine the influence of thyrotropin (TSH) on the thyroglobulin (Tgb) mRNA content, the latter was evaluated in the cytoplasm of hog thyroid cells cultured in the absence (control cells) or presence of TSH. The Tgb mRNA levels were determined by, (i) kinetics of hybridization to sheep Tgb cDNA, (ii) capacity of cod...

journal_title：Nucleic acids research

authors： Chebath J,Chabaud O,Mauchamp J

更新日期：1979-07-25 00:00:00

abstract：:Genomic meta-analysis to combine relevant and homogeneous studies has been widely applied, but the quality control (QC) and objective inclusion/exclusion criteria have been largely overlooked. Currently, the inclusion/exclusion criteria mostly depend on ad-hoc expert opinion or naïve threshold by sample size or platfo...

journal_title：Nucleic acids research

authors： Kang DD,Sibille E,Kaminski N,Tseng GC

更新日期：2012-01-01 00:00:00

abstract：:The active and inactive state of transcription factors in growing cells is usually directed by allosteric physicochemical signals or metabolites, which are in turn either produced in the cell or obtained from the environment by the activity of the products of effector genes. To understand the regulatory dynamics and t...

journal_title：Nucleic acids research

authors： Janga SC,Salgado H,Martínez-Antonio A,Collado-Vides J

更新日期：2007-01-01 00:00:00

abstract：:The ciliate Tetrahymena thermophila is an important eukaryotic model organism that has been used in pioneering studies of general phenomena, such as ribozymes, telomeres, chromatin structure and genome reorganization. Recent work has shown that Tetrahymena has many classes of small RNA molecules expressed during veget...

journal_title：Nucleic acids research

authors： Andersen KL,Nielsen H

更新日期：2012-02-01 00:00:00

abstract：:The advances of next-generation sequencing technology have facilitated metagenomics research that attempts to determine directly the whole collection of genetic material within an environmental sample (i.e. the metagenome). Identification of genes directly from short reads has become an important yet challenging probl...

journal_title：Nucleic acids research

authors： Rho M,Tang H,Ye Y

更新日期：2010-11-01 00:00:00

abstract：:Lnc2Meth (http://www.bio-bigdata.com/Lnc2Meth/), an interactive resource to identify regulatory relationships between human long non-coding RNAs (lncRNAs) and DNA methylation, is not only a manually curated collection and annotation of experimentally supported lncRNAs-DNA methylation associations but also a platform t...

journal_title：Nucleic acids research

authors： Zhi H,Li X,Wang P,Gao Y,Gao B,Zhou D,Zhang Y,Guo M,Yue M,Shen W,Ning S,Jin L,Li X

更新日期：2018-01-04 00:00:00

abstract：:The SeqA protein binds hemi-methylated GATC sites and forms structures that sequester newly replicated origins and trail the replication forks. Cells that lack SeqA display signs of replication fork disintegration. The broken forks could arise because of over-initiation (the launching of too many forks) or lack of dyn...

journal_title：Nucleic acids research

authors： Pedersen IB,Helgesen E,Flåtten I,Fossum-Raunehaug S,Skarstad K

更新日期：2017-06-20 00:00:00

abstract：:Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B ...

journal_title：Nucleic acids research

authors： Rickert RC,Roes J,Rajewsky K

更新日期：1997-03-15 00:00:00

abstract：:Signals setting the level of transcription of the tRNA-tufB operon have been studied by deletion mapping. TufB transcription was measured in vivo with plasmid-borne tRNA-tufB:galk operon fusions. Removal of the sequences from -133 to -58 with respect to the transcription start point, results in a 90% decrease of tufB ...

journal_title：Nucleic acids research

authors： van Delft JH,Mariñon B,Schmidt DS,Bosch L

更新日期：1987-11-25 00:00:00

abstract：:Several restriction endonuclease fragments isolated from highly repetitive satellite DNA of the chromatin eliminating nematode Ascaris lumbricoides var. suum have been cloned. Each type of restriction fragment corresponds to a different variant of the same related ancestral sequence. These variants differ by small del...

journal_title：Nucleic acids research

authors： Müller F,Walker P,Aeby P,Neuhaus H,Felder H,Back E,Tobler H

更新日期：1982-12-11 00:00:00

abstract：:The 5'-cap of spliceosomal small nuclear RNAs, some small nucleolar RNAs and of telomerase RNA was found to be hypermethylated in vivo. The Trimethylguanosine Synthase 1 (TGS1) mediates this conversion of the 7-methylguanosine-cap to the 2,2,7-trimethylguanosine (m(3)G)-cap during maturation of the RNPs. For mammalian...

journal_title：Nucleic acids research

authors： Monecke T,Dickmanns A,Ficner R

更新日期：2009-07-01 00:00:00

abstract：:The role of TATA elements in the expression of a mammalian promoter was investigated in the fission yeast Schizosaccharomyces pombe, by studying the human adenovirus E2-early promoter. This is a unique dual promoter with two nonconsensus TATA elements directing transcription from two cap sites, +1 and -26. A sequence ...

journal_title：Nucleic acids research

authors： Swaminathan S,Malhotra P,Manohar CF,Dhar R,Thimmapaya B

更新日期：1993-06-11 00:00:00

abstract：:ColE1 derivative plasmids were constructed in which the natural promoter that primes replication or, in addition, the region coding for the RNA I control element had been deleted. In all of these molecules priming of the origin was effected by read-through transcription from constitutive or inducible (lacUV5) promoter...

journal_title：Nucleic acids research

authors： Panayotatos N

更新日期：1984-03-26 00:00:00

abstract：:The rolling circle DNA replication structures generated by the in vitro phage T4 replication system were analyzed using two-dimensional agarose gels. Replication structures were generated in the presence or absence of T4 primase (gp61), permitting the analysis of replication forks with either duplex or single-stranded...

journal_title：Nucleic acids research

authors： Belanger KG,Mirzayan C,Kreuzer HE,Alberts BM,Kreuzer KN

更新日期：1996-06-01 00:00:00

abstract：:Mobile introns and inteins self-propagate by 'homing', a gene conversion process initiated by site-specific homing endonucleases. The VMA intein, which encodes the PI-SceI endonuclease in Saccharomyces cerevisiae, is present in several different yeast strains. Surprisingly, a wild wine yeast (DH1-1A) contains not only...

journal_title：Nucleic acids research

authors： Gimble FS

更新日期：2001-10-15 00:00:00

abstract：:Gene duplications are a major source of evolutionary innovations. Understanding the functional divergence of duplicates and their role in genetic robustness is an important challenge in biology. Previously, analyses of genetic robustness were primarily focused on duplicates essentiality and epistasis in several labora...

journal_title：Nucleic acids research

authors： Plata G,Vitkup D

更新日期：2014-02-01 00:00:00

abstract：:Southern blot analyses using reduced stringency hybridization conditions have been employed to search for sequence homologies between rodent VL30 genes and murine leukemia virus (MuLV) proviruses. These constitute two classes of transposon-like elements previously believed to be genetically unrelated. Our results demo...

journal_title：Nucleic acids research

authors： Giri CP,Hodgson CP,Elder PK,Courtney MG,Getz MJ

更新日期：1983-01-25 00:00:00

abstract：:RNA-based therapeutics, i.e. the utilization of synthetic RNA molecules to alter cellular functions, have the potential to address targets which are currently out of scope for traditional drug design pipelines. This potential however hinges on the ability to selectively deliver and internalize therapeutic RNAs into ce...

journal_title：Nucleic acids research

authors： Wang Y,Hoinka J,Liang Y,Adamus T,Swiderski P,Przytycka TM

更新日期：2018-09-19 00:00:00

abstract：:Because of their high nucleotide sequence homology, the specific detection of mRNA transcripts of individual members of a gene family presents certain problems. Here we apply and defend the single nucleotide primer extension (SNuPE) as a simple, specific, and highly quantitative assay for this purpose. The method dist...

journal_title：Nucleic acids research

authors： Lombardo AJ,Brown GB

更新日期：1996-12-01 00:00:00

abstract：:A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing S...

journal_title：Nucleic acids research

authors： Pyle LE,Finch LR

更新日期：1988-07-11 00:00:00

abstract：:Transcription factors are important gene regulators with distinctive roles in development, cell signaling and cell cycling, and they have been associated with many diseases. The ConTra v3 web server allows easy visualization and exploration of predicted transcription factor binding sites (TFBSs) in any genomic region ...

journal_title：Nucleic acids research

authors： Kreft L,Soete A,Hulpiau P,Botzki A,Saeys Y,De Bleser P

更新日期：2017-07-03 00:00:00

abstract：:M13 RF IV DNA may be prepared in vitro to contain phosphorothioate-modified internucleotidic linkages in the (-)strand only. Certain restriction enzymes react with this modified DNA to hydrolyze the (+)strand exclusively when a phosphorothioate linkage occurs at the normal cleavage point in the (-)strand. The reaction...

journal_title：Nucleic acids research

authors： Taylor JW,Ott J,Eckstein F

更新日期：1985-12-20 00:00:00

abstract：:The FREP database (http://facts.gsc.riken.go.jp/FREP/) contains 31 396 RepeatMasker-identified non-redundant variant repeat sequences derived from 16,527 mouse cDNAs with protein-coding potential. The repeats were computationally associated with potential effects on transcriptional variation, translation, protein func...

journal_title：Nucleic acids research

authors： Nagashima T,Matsuda H,Silva DG,Petrovsky N,Konagaya A,Schönbach C,Kasukawa T,Arakawa T,Carninci P,Kawai J,Hayashizaki Y,RIKEN GER Group.,Genome Science Laoratory Members.

更新日期：2004-01-01 00:00:00