Elk-1 can recruit SRF to form a ternary complex upon the serum response element.


:The initial genomic response to serum growth factors is the transcriptional activation of a set of immediate-early genes. Serum-induced transcriptional activation of several of these genes involves the formation of a ternary complex that includes the serum response factor (SRF), a 62 kDa ternary complex factor (TCF) and a serum response element (SRE). TCF alone does not bind the SRE of the protooncogene c-fos, but requires the prior assembly of the SRF-SRE binary complex for it to be recruited into a ternary complex. Here we show that this SRF-SRE binary complex is not an obligatory prerequisite for the formation of a serum responsive ternary complex. We demonstrate that Elk-1, which has properties of TCF can recruit SRF into a ternary complex on elements that do not support formation of the SRF-DNA binary complex. We also show that for two immediate-early genes, pip92 and nur77, formation of the ternary complex may occur without the prior assembly of SRF-DNA binary complex. Finally, we show that the ability of different sequences to support formation of Elk-l-SRF-DNA ternary complex in vitro correlates with their ability to respond to serum growth factors in vivo. Our results suggest that a much broader range of DNA sequences than the consensus SRF and TCF binding sites can support ternary complex formation, and by inference, serum induction. Possible implications of these results are discussed.


Nucleic Acids Res


Nucleic acids research


Latinkić BV,Zeremski M,Lau LF




Has Abstract


1996-04-01 00:00:00














  • Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection.

    abstract::Multiplexed tandem PCR (MT-PCR) is a process for highly multiplexed gene expression profiling. In the first step, multiple primer pairs are added to the RNA to be analysed together with reverse transcriptase and Taq DNA polymerase. Following reverse transcription, the multiplexed amplicons are simultaneously amplified...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Stanley KK,Szewczuk E

    更新日期:2005-11-24 00:00:00

  • Highly selective retrieval of accurate DNA utilizing a pool of in situ-replicated DNA from multiple next-generation sequencing platforms.

    abstract::Scalable and cost-effective production of error-free DNA is critical to meet the increased demand for such DNA in the field of biological science. Methods based on 'Dial-out PCR' have enabled the high-throughput error-free DNA synthesis from a microarray-synthesized DNA pool by labeling with retrieval PCR tags, and re...

    journal_title:Nucleic acids research

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    authors: Lim H,Cho N,Ahn J,Park S,Jang H,Kim H,Han H,Lee JH,Bang D

    更新日期:2018-04-20 00:00:00

  • Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.

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    journal_title:Nucleic acids research

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    authors: Neylon C

    更新日期:2004-02-27 00:00:00

  • A nucleobase-binding pocket in a viral RNA-dependent RNA polymerase contributes to elongation complex stability.

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    authors: Korada SK,Johns TD,Smith CE,Jones ND,McCabe KA,Bell CE

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    journal_title:Nucleic acids research

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sen GC,Ghosh HP

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    更新日期:2018-04-06 00:00:00