Abstract:
:A single base change in 16S rRNA (C726 to G) has previously been shown to have a dramatic effect on protein synthesis in E. coli (1). This paper more specifically details the effects of the mutation on mRNA binding and translation-termination. The in vitro technique of toeprinting (2) was used to demonstrate that 30S subunits containing the mutation 726G had an altered binding affinity for mRNA by comparison to the wild type. In addition, expression of the mutant ribosomes in vivo resulted in exclusive suppression of the UGA nonsense codon. This effect was supported by in vitro studies that showed the mutant ribosomes to have an altered binding affinity for Release Factor-2.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Prescott CD,Göringer HUdoi
10.1093/nar/18.18.5381subject
Has Abstractpub_date
1990-09-25 00:00:00pages
5381-6issue
18eissn
0305-1048issn
1362-4962journal_volume
18pub_type
杂志文章abstract::The endonuclease activity within the influenza virus cap-snatching process is a proven therapeutic target. The anti-influenza drug baloxavir is highly effective, but is associated with resistance mutations that threaten its clinical efficacy. The endonuclease resides within the N-terminal domain of the PA subunit (PAN...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1294
更新日期:2021-01-19 00:00:00
abstract::A growing body of evidence suggests that siRNA could generate off-target effects through different mechanisms. However, the full impact of off-target gene regulation on phenotypic induction and accordingly on data interpretation in the context of large-scale siRNA library screen has not been reported. Here we report o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki762
更新日期:2005-08-09 00:00:00
abstract::MiRNAs post-transcriptionally regulate gene expression by recruiting the miRNA-induced silencing complex (miRISC) to target mRNAs. However, the mechanisms by which miRISC components are maintained at appropriate levels for proper function are largely unknown. Here, we demonstrate that Caenorhabditis elegans TEG-1 regu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw836
更新日期:2017-02-17 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm096
更新日期:2007-01-01 00:00:00
abstract::Post-translational modifications (PTMs) regulate many aspects of protein function and are indispensable for the spatio-temporal regulation of cellular processes. The proteome-wide identification of PTM targets has made significant progress in recent years, as has the characterization of their writers, readers, modifie...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkv1383
更新日期:2016-02-18 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp477
更新日期:2009-07-01 00:00:00
abstract::Protein-RNA interactions play essential roles in a number of regulatory mechanisms for gene expression such as RNA splicing, transport, translation and post-transcriptional control. As the number of available protein-RNA complex 3D structures has increased, it is now possible to statistically examine protein-RNA inter...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl819
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.5.1103
更新日期:2002-03-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.1.166
更新日期:1996-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1995-03-11 00:00:00
abstract::The structure and organisation of the human N-ras gene has been determined by analysing cDNA clones derived from the two main mRNA transcripts. One clone in particular is 4.1 Kb long and originates from the larger (4.3 Kb) message. Sequence analysis of this clone has revealed that the N-ras gene consists of seven exon...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.14.5255
更新日期:1985-07-25 00:00:00
abstract::Two models have been proposed for triggering release of the lagging strand polymerase at the replication fork, enabling cycling to the primer for the next Okazaki fragment--either collision with the 5'-end of the preceding fragment (collision model) or synthesis of a new primer by primase (signaling model). Specific p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1098
更新日期:2014-02-01 00:00:00
abstract::The mRNA export complex TREX (TREX) is known to contain Aly, UAP56, Tex1 and the THO complex, among which UAP56 is required for TREX assembly. Here, we systematically investigated the role of each human TREX component in TREX assembly and its association with the mRNA. We found that Tex1 is essentially a subunit of th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1188
更新日期:2013-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.9.3439
更新日期:1978-09-01 00:00:00
abstract::OrthoDB is a comprehensive catalog of orthologs, genes inherited by extant species from a single gene in their last common ancestor. In 2016 OrthoDB reached its 9th release, growing to over 22 million genes from over 5000 species, now adding plants, archaea and viruses. In this update we focused on usability of this f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1119
更新日期:2017-01-04 00:00:00
abstract::Thyroid hormone receptors (TRs) are members of the nuclear hormone receptor superfamily, which act as transcription factors upon binding to specific DNA sequences called thyroid hormone (T3) response elements (TREs). Such elements are found in the upstream regulatory region of promoters as well as in intragenic sequen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.9.1774
更新日期:1997-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1128
更新日期:2011-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.21.9947
更新日期:1988-11-11 00:00:00
abstract::Enzymatic hydrolysis and base analysis by high performance liquid chromatography showed that mouse satellite DNA had 30-50% less 5-methylcytosine in sperm than in somatic tissue (1.59 mols % vs 2.40-3.11 mols %). Maxam-Gilbert sequencing and analysis of the intensity of the cytosine bands indicated that the level of m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.11.3969
更新日期:1985-06-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh056
更新日期:2004-04-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp446
更新日期:2009-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.20.4626
更新日期:1998-10-15 00:00:00
abstract::DNA methylation and chromatin DNaseI sensitivity were analyzed in and adjacent to D4Z4 repeat arrays, which consist of 1 to approximately 100 tandem 3.3-kb units at subtelomeric 4q and 10q. D4Z4 displayed hypomethylation in some cancers and hypermethylation in others relative to normal tissues. Surprisingly, in cancer...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn055
更新日期:2008-04-01 00:00:00
abstract::Sequences from -3500 to +1365 of the chicken histone H5 gene have been analyzed for the presence of cis-acting elements in H5 expressing (transformed CFU-E) and non-expressing cells (fibroblasts). The region from -3500 to -115 had little effect on transcription. Proximal upstream sequences contain a negative element (...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.18.7495
更新日期:1989-09-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.8.999
更新日期:1974-08-01 00:00:00
abstract::The sequence of the 356 nucleotide residues of chrysanthemum stunt viroid (CSV) has been determined. Overlapping linear viroid fragments were obtained by partial ribonuclease digestion, radiolabelled in vitro at their 5'-ends, and sequenced using partial enzymic cleavage methods. Of the CSV sequence, 69% is contained ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.12.2741
更新日期:1981-06-25 00:00:00
abstract::The laboratory mouse is the premier animal model for studying human biology because all life stages can be accessed experimentally, a completely sequenced reference genome is publicly available and there exists a myriad of genomic tools for comparative and experimental research. In the current era of genome scale, dat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1115
更新日期:2013-01-01 00:00:00