Abstract：
:We have previously described a partial Drosophila cDNA, clone P19, which bears homology to members of the RNA recognition motif (RRM) family of proteins [Haynes et al. (1987) Proc. Natl. Acad. Sci. USA, 84, 1819-1823]. RNA binding as well as involvement in RNA processing has been demonstrated for some RRM proteins. We report here the further characterization of P19, which we renamed cabeza (caz). caz is located on the X chromosome at position 14B. Using Northern analysis, at least four transcripts from the caz gene were observed at varying levels during development. caz mRNA and protein are enriched in the brain and central nervous system during embryogenesis. In addition, the protein is enriched in the adult head. UV crosslinking was used to demonstrate in vitro RNA binding activity for full-length recombinant caz protein and for the caz RRM domain. Sequence analysis revealed caz is related to two human genes, EWS and TLS, which are involved in chromosomal translocations. The fusion of EWS and TLS to other cellular genes results in sarcoma formation. In addition to their overall structural organization and sequence similarity, these three genes share an RRM which is divergent from typical RRMs. Therefore, it appears that these genes constitute a new sub-family of RNA binding proteins.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Stolow DT,Haynes SRdoi
10.1093/nar/23.5.835subject
Has Abstractpub_date
1995-03-11 00:00:00pages
835-43issue
5eissn
0305-1048issn
1362-4962pii
4t0706journal_volume
23pub_type
杂志文章abstract：:Cells continuously monitor protein synthesis to prevent accumulation of aberrant polypeptides. Insufficient capacity of cellular degradative systems, chaperone shortage or high levels of mistranslation by ribosomes can result in proteotoxic stress and endanger proteostasis. One of the least explored reasons for mistra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa068
更新日期：2020-04-17 00:00:00
abstract：:Short DNA sequence motifs have been identified in viral and cellular enhancers which represent the binding sites for a variety of trans- acting factors. One such HeLa cell factor, EBP1, has been purified and shown to bind to sequences in the SV40 enhancer. The PRDII element in the human beta-interferon gene regulatory...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.2.499
更新日期：1989-01-25 00:00:00
abstract：:Increasing experimental evidence shows a prominent role of histone modifications in the coordinated control of gene expression in the human malaria parasite Plasmodium falciparum. The search for the histone-mark-reading machinery that translates histone modifications into biological processes, such as formation of het...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp115
更新日期：2009-05-01 00:00:00
abstract：:Mitochondrial transcription factor A (TFAM) stimulates transcription from mitochondrial DNA (mtDNA) promoters in vitro and in organello. To investigate whether changes of TFAM levels also modulate transcription and replication in situ, the protein was transiently overexpressed in cultured cells. Mitochondrial mRNAs we...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh921
更新日期：2004-11-16 00:00:00
abstract：:Phosphoprotein-binding domains (PPBDs) mediate many important cellular and molecular processes. Ten PPBDs have been known to exist in the human proteome, namely, 14-3-3, BRCT, C2, FHA, MH2, PBD, PTB, SH2, WD-40 and WW. PepCyber:P approximately PEP is a newly constructed database specialized in documenting human PPBD-c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm854
更新日期：2008-01-01 00:00:00
abstract：:The Aspergillus Genome Database (AspGD) is an online genomics resource for researchers studying the genetics and molecular biology of the Aspergilli. AspGD combines high-quality manual curation of the experimental scientific literature examining the genetics and molecular biology of Aspergilli, cutting-edge comparativ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp751
更新日期：2010-01-01 00:00:00
abstract：:We report the use of an engineered ribozyme to produce a circular human exon in vitro. Specifically, we have designed a derivative of a yeast self-splicing group II intron that is able to catalyze the formation of a circular exon encoding the first kringle domain (K1) of the human tissue plasminogen activator protein....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.24.5085
更新日期：1997-12-15 00:00:00
abstract：:We show that in mouse myoblasts the MyoD1 promoter is highly stimulated by MyoD1 expression, suggesting that it is controlled by a positive feedback loop. Using deletion and mutation analyses, we identified the targets for MyoD1 promoter autoregulation as the two proximal E-boxes located close to the MyoD1 core promot...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.12.2234
更新日期：1994-06-25 00:00:00
abstract：:By putting heterologous genomic regulatory systems into contact, chromosome addition lines derived from interspecific or intergeneric crosses allow the investigation of transcriptional regulation in new genomic environments. Here, we report the transcriptional and epigenetic adaptation of stably inherited alien maize ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky209
更新日期：2018-06-01 00:00:00
abstract：:Post-transcriptional regulation and the formation of mRNA 3' ends are crucial for gene expression in eukaryotes. Interspecies conservation of many sequences within 3'UTRs reveals selective constraint due to similar function. To study the pattern of conservation within 3'UTRs, we compiled and aligned 50 sets of complet...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg751
更新日期：2003-09-15 00:00:00
abstract：:Global run-on sequencing (GRO-seq) is a recent addition to the series of high-throughput sequencing methods that enables new insights into transcriptional dynamics within a cell. However, GRO-sequencing presents new algorithmic challenges, as existing analysis platforms for ChIP-seq and RNA-seq do not address the uniq...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1237
更新日期：2014-02-01 00:00:00
abstract：:Promoter design remains one of the most important considerations in metabolic engineering and synthetic biology applications. Theoretically, there are 450 possible sequences for a 50-nt promoter, of which naturally occurring promoters make up only a small subset. To explore the vast number of potential sequences, we r...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa325
更新日期：2020-07-09 00:00:00
abstract：:Primase synthesizes decaribonucleotides for priming of lagging and possibly leading strand synthesis at a replication fork. The sites of initiation by purified mouse primase were shown to be highly specific within the SV40 origin of replication. This study further examines the role of the 27-bp inverted repeat in the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.5.1953
更新日期：1989-03-11 00:00:00
abstract：:The reactivities of the two cysteine thiol groups of calf thymus F3 histone were investigated using 5,5'-dithiobis-[2- nitrobenzoic acid], (DTNB). In isolated histone, both thiol groups were available for reaction. However, analysis of reaction profiles of native deoxyribonucleohistone, (DNH), in various solvent condi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.2.203
更新日期：1974-02-01 00:00:00
abstract：:We report the first isolation and characterisation of a human Ig V lambda gene. The gene was isolated from a recombinant phage library of human chromosome 22 using a mouse Ig lambda cDNA as probe. DNA sequence analysis predicts a short leader peptide interrupted by an intron of 88 nucleotides, and a mature polypeptide...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.17.6647
更新日期：1984-09-11 00:00:00
abstract：:We have formed complexes of relaxed closed circular Col E1 DNA with various combinations of histones, and examined the effects of treating the complexes with nicking-closing enzyme. Germond et al (1) have shown that when a mixture of the four core histones of the nucleosome (HIA, H2B, H3 and H4) is used in such an exp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.5.1159-a
更新日期：1977-01-01 00:00:00
abstract：:DNA nick can be used as a design motif in programming the shape and reconfigurable deformation of synthetic DNA nanostructures, but its mechanical properties have rarely been systematically characterized at the level of base sequences. Here, we investigated sequence-dependent mechanical properties of DNA nicks through...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1189
更新日期：2019-01-10 00:00:00
abstract：:In order to further elucidate the mechanism of tRNA methylase-tRNA intreaction the methylation of some individual tRNAs separately and by pairs was performed. In conditions of tRNA excess the methylation rates of positionally analogous nucleotides in tRNA molecules are not summed up when two substrates are simultaneou...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.8.2079
更新日期：1976-08-01 00:00:00
abstract：:Exome sequencing strategy is promising for finding novel mutations of human monogenic disorders. However, pinpointing the casual mutation in a small number of samples is still a big challenge. Here, we propose a three-level filtration and prioritization framework to identify the casual mutation(s) in exome sequencing ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1257
更新日期：2012-04-01 00:00:00
abstract：:The unknown modified nucleotide G*, isolated from both Schizosaccharomyces pombe and Torulopsis utilis initiator tRNAs(Met), has been identified as an O-ribosyl-(1"----2')-guanosine-5"-phosphate, called Gr(p), by means of HPLC, UV-absorption, mass spectrometry and periodate oxidation procedures. By comparison with the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.19.5199
更新日期：1991-10-11 00:00:00
abstract：:The opening of DNA double strands is extremely relevant to several biological functions, such as replication and transcription or binding of specific proteins. Such opening phenomenon is particularly sensitive to the aqueous solvent conditions in which the DNA molecule is dispersed, as it can be observed by considerin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1206
更新日期：2013-02-01 00:00:00
abstract：:IntAct is an open source database and software suite for modeling, storing and analyzing molecular interaction data. The data available in the database originates entirely from published literature and is manually annotated by expert biologists to a high level of detail, including experimental methods, conditions and ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl958
更新日期：2007-01-01 00:00:00
abstract：:We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.7.1521
更新日期：1991-04-11 00:00:00
abstract：:We have constructed a stable cell line, human embryonal kidney 293M+, containing a lacZ reporter gene controlled by an in vitro methylated hormone-responsive enhancer. Methylation of the enhancer-promoter abolishes lacZ expression controlled by ponasterone A (an analogue of ecdysone). Ponasterone A-induced expression ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.17.3603
更新日期：2001-09-01 00:00:00
abstract：:The unwinding of supercoiled phi X174 RFI DNA induced by the tumorigenic (+) and non-tumorigenic (-) enantiomers of trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) has been investigated by agarose slab-gel and ethidium titration tube gel electrophoresis. The differences in adduct conformat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.23.6167
更新日期：1992-12-11 00:00:00
abstract：:A list of DNA sequences cloned from the human genome is presented. Intended as a guide to clone availability, this list includes published reports of cDNA, genomic and synthetic clones comprising gene and pseudogene sequences, uncharacterised DNA segments and repetitive DNA elements. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.suppl.r403
更新日期：1988-01-01 00:00:00
abstract：:R.E.D. Server is a unique, open web service, designed to derive non-polarizable RESP and ESP charges and to build force field libraries for new molecules/molecular fragments. It provides to computational biologists the means to derive rigorously molecular electrostatic potential-based charges embedded in force field l...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr288
更新日期：2011-07-01 00:00:00
abstract：:Augmented biological activity in vitro has been demonstrated in oligonucleotides (oligos) modified to provide nuclease resistance, to enhance cellular uptake or to increase target affinity. How chemical modification affects the duration of effect of an oligo with potent activity has not been investigated directly. We ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.6.977
更新日期：1994-03-25 00:00:00
abstract：:A repeated sequence element which is located about 200 nucleotides upstream from the protein-coding portion of the muscle actin gene (probably within a large 5' intron) in the genome of the sea urchin, Strongylocentrotus purpuratus has been characterized, and shown to contain the sequence features which indicate that ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.4.1431
更新日期：1988-02-25 00:00:00
abstract：:The gene encoding myelin basic protein (MBP) contains multiple activator sequences spanning upstream of its transcriptional initiation site which differentially promote transcription in glial cells. The proximal activator sequence, designated MB1, activates transcription in a glial cell type specific manner. This sequ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.3.545
更新日期：1992-02-11 00:00:00
