Abstract:
:The nematode Caenorhabditis elegans contains a small family of vitellogenin genes which is expressed abundantly, but only in the intestine of the adult hermaphrodite worm. In order to identify possible regulatory elements, we have sequenced the DNA surrounding the 5' ends of five of the six genes. Contained within regions which have largely diverged from one another, two different heptameric sequences are found repeated within the first 200 bp upstream of each of the genes. The first sequence, TGTCAAT, is present as a perfect heptamer at least once upstream of each gene. It is repeated in both orientations four to six times in each 5' flanking region, allowing a one-base mismatch. The second sequence, CTGATAA, is also present as a perfect heptamer in a restricted region upstream of each gene. These two sequence elements may be involved in regulation of the vitellogenin genes. Remarkably, the CTGATAA sequence is present in a similar location in the promoter regions of vertebrate vitellogenin genes. In fact, our data reveal a surprising degree of similarity between the nematode and vertebrate vitellogenins.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Spieth J,Denison K,Kirtland S,Cane J,Blumenthal Tdoi
10.1093/nar/13.14.5283subject
Has Abstractpub_date
1985-07-25 00:00:00pages
5283-95issue
14eissn
0305-1048issn
1362-4962journal_volume
13pub_type
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