Abstract:
:A differential effect is found of various bivalent cations (Ba2+, Ca2+, Mg2+, Cd2+, Co2+, Mn2+, Ni2+, Zn2+ and Hg2+) on stability of intermolecular Py-Pu-Pu triplex with different sequence of base triads. Ca2+, Mg2+, Cd2+, Co2+, Mn2+, Ni2+ and Zn2+ do stabilize the d(C)n d(G)n d(G)n triplex whereas Ba2+ and Hg2+ do not. Ba2+, Ca2+, Mg2+ and Hg2+ destabilize the d(TC)n d(GA)n d(AG)n triplex whereas Cd2+, Co2+, Mn2+, Ni2+ and Zn2+ stabilize it. The complexes we observe are rather stable because they do not dissociate during time of gel electrophoresis in the co-migration experiments. Chemical probing experiments with dimethyl sulfate as a probe indicate that an arbitrary homopurine-homopyrimidine sequence forms triplex with corresponding purine oligonucleotide in the presence of Mn2+ or Zn2+, but not Mg2+. In the complex the purine oligonucleotide has antiparallel orientation with respect to the purine strand of the duplex. Specifically, we have shown the formation of the Py-Pu-Pu triplex in a fragment of human papilloma virus HPV-16 in the presence of Mn2+.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Malkov VA,Voloshin ON,Soyfer VN,Frank-Kamenetskii MDdoi
10.1093/nar/21.3.585subject
Has Abstractpub_date
1993-02-11 00:00:00pages
585-91issue
3eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract::Hfq, a bacterial RNA-binding protein, was recently shown to contain the Sm1 motif, a characteristic of Sm and LSm proteins that function in RNA processing events in archaea and eukaryotes. In this report, comparative structural modeling was used to predict a three-dimensional structure of the Hfq core sequence. The pr...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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doi:10.1093/nar/20.24.6687
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.18.5381
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.5.761
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journal_title:Nucleic acids research
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更新日期:2006-03-17 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1132
更新日期:2013-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-10-13 00:00:00
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journal_title:Nucleic acids research
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更新日期:1982-02-11 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2013-04-01 00:00:00