A method of one-step enzyme labelling of short oligonucleotide probes for filter hybridisation.

Abstract:

:Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detection of hybridisation signal is linear for two orders of magnitude of target dilution. It is shown to be comparable in sensitivity with standard procedures and with radioactive detection. The method is quick, simple and has potential for automation of large-scale oligo-nucleotide hybridisation and multiplex sequencing.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Guerasimova A,Ivanov I,Lehrach H

doi

10.1093/nar/27.2.703

subject

Has Abstract

pub_date

1999-01-15 00:00:00

pages

703-5

issue

2

eissn

0305-1048

issn

1362-4962

pii

gkc154

journal_volume

27

pub_type

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