Abstract:
:Fully protected diastereoisomers of deoxyguanylyl (3' leads to 5') deoxyadenosine stereospecifically labelled on phosphorus with oxygen-18 have been synthesized by oxidation of phosphite triester intermediates in the presence of 18O-labelled water. The diastereoisomers have been chromatographically separated and their absolute configuration at phosphorus determined. (Rp)-[18O]deoxyguanylyl (3' leads to 5')deoxyadenosine has been prepared by complete deprotection of the parent diastereoisomer of the Sp configuration. Methylation of the former compound permits assignment of the absolute configurations of the methyl esters of N1-methyldeoxyguanylyl (3' leads to 5') N1-methyldeoxyadenosine.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Potter BV,Eckstein F,Uznański Bdoi
10.1093/nar/11.20.7087subject
Has Abstractpub_date
1983-10-25 00:00:00pages
7087-103issue
20eissn
0305-1048issn
1362-4962journal_volume
11pub_type
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