当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > DnaG interacts with a linker region that joins the N- and C-domains of DnaB and induces the formation of 3-fold symmetric rings.

DnaG interacts with a linker region that joins the N- and C-domains of DnaB and induces the formation of 3-fold symmetric rings.

Abstract：

:Loading of the replicative ring helicase onto the origin of replication (oriC) is the final outcome of a well coordinated series of events that collectively constitute a primosomal cascade. Once the ring helicase is loaded, it recruits the primase and signals the switch to the polymerization mode. The transient nature of the helicase-primase (DnaB-DnaG) interaction in the Escherichia coli system has hindered our efforts to elucidate its structure and function. Taking advantage of the stable DnaB-DnaG complex in Bacillus stearothermophilus, we have reviewed conflicting mutagenic data from other bacterial systems and shown that DnaG interacts with the flexible linker that connects the N- and C-terminal domains of DnaB. Furthermore, atomic force microscopy (AFM) imaging experiments show that binding of the primase to the helicase induces predominantly a 3-fold symmetric morphology to the hexameric ring. Overall, three DnaG molecules appear to interact with the hexameric ring helicase but a small number of complexes with two and even one DnaG molecule bound to DnaB were also detected. The structural/functional significance of these data is discussed and a speculative structural model for this complex is suggested.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Thirlway J,Turner IJ,Gibson CT,Gardiner L,Brady K,Allen S,Roberts CJ,Soultanas P

doi

10.1093/nar/gkh628

keywords:

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

2977-86

issue

10

eissn

0305-1048

issn

1362-4962

pii

32/10/2977

journal_volume

32

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • A novel POU homeodomain gene specifically expressed in cells of the developing mammalian nervous system.

    abstract：:We report the isolation of a novel human POU domain encoding gene named RDC-1. The POU domain of the RDC-1 encoded protein is highly related to the POU domain potentially encoded by the rat brain-3 sequence and to that of the Drosophila I-POU protein; outside of the POU region, RDC-1 is unrelated to any previously cha...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.18.4919

    authors： Collum RG,Fisher PE,Datta M,Mellis S,Thiele C,Huebner K,Croce CM,Israel MA,Theil T,Moroy T

    更新日期：1992-09-25 00:00:00

  • Automated Gene Ontology annotation for anonymous sequence data.

    abstract：:Gene Ontology (GO) is the most widely accepted attempt to construct a unified and structured vocabulary for the description of genes and their products in any organism. Annotation by GO terms is performed in most of the current genome projects, which besides generality has the advantage of being very convenient for co...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg582

    authors： Hennig S,Groth D,Lehrach H

    更新日期：2003-07-01 00:00:00

  • TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells.

    abstract：:PSF (a.k.a. SFPQ) is a ubiquitously expressed, essential nuclear protein with important roles in DNA damage repair and RNA biogenesis. In stimulated T cells, PSF binds to and suppresses the inclusion of CD45 exon 4 in the final mRNA; however, in resting cells, TRAP150 binds PSF and prevents access to the CD45 RNA, tho...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv816

    authors： Yarosh CA,Tapescu I,Thompson MG,Qiu J,Mallory MJ,Fu XD,Lynch KW

    更新日期：2015-10-15 00:00:00

  • Flexible expressed region analysis for RNA-seq with derfinder.

    abstract：:Differential expression analysis of RNA sequencing (RNA-seq) data typically relies on reconstructing transcripts or counting reads that overlap known gene structures. We previously introduced an intermediate statistical approach called differentially expressed region (DER) finder that seeks to identify contiguous regi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw852

    authors： Collado-Torres L,Nellore A,Frazee AC,Wilks C,Love MI,Langmead B,Irizarry RA,Leek JT,Jaffe AE

    更新日期：2017-01-25 00:00:00

  • The GW/WG repeats of Drosophila GW182 function as effector motifs for miRNA-mediated repression.

    abstract：:The control of messenger RNA (mRNA) function by micro RNAs (miRNAs) in animal cells requires the GW182 protein. GW182 is recruited to the miRNA repression complex via interaction with Argonaute protein, and functions downstream to repress protein synthesis. Interaction with Argonaute is mediated by GW/WG repeats, whic...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq501

    authors： Chekulaeva M,Parker R,Filipowicz W

    更新日期：2010-10-01 00:00:00

  • Position-dependent effects of regioisomeric methylated adenine and guanine ribonucleosides on translation.

    abstract：:Reversible methylation of the N6 or N1 position of adenine in RNA has recently been shown to play significant roles in regulating the functions of RNA. RNA can also be alkylated upon exposure to endogenous and exogenous alkylating agents. Here we examined how regio-specific methylation at the hydrogen bonding edge of ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkx515

    authors： You C,Dai X,Wang Y

    更新日期：2017-09-06 00:00:00

  • The base pairing properties of 8-aza-7-deaza-2'-deoxyisoguanosine and 7-halogenated derivatives in oligonucleotide duplexes with parallel and antiparallel chain orientation.

    abstract：:The base pairing properties of oligonucleotide duplexes containing 8-aza-7-deaza-2'-deoxyisoguanosine, its 7-bromo or its 7-iodo derivative are described. The nucleosides were synthesized on a convergent route, protected and converted into phosphoramidites. Oligonucleotides were prepared on a solid-phase and were hybr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg926

    authors： Seela F,Kröschel R

    更新日期：2003-12-15 00:00:00

  • Phosphorylation of an intrinsically disordered region of Ets1 shifts a multi-modal interaction ensemble to an auto-inhibitory state.

    abstract：:Multi-modal interactions are frequently observed in intrinsically disordered regions (IDRs) of proteins upon binding to their partners. In many cases, post-translational modifications in IDRs are accompanied by coupled folding and binding. From both molecular simulations and biochemical experiments with mutational stu...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkx1297

    authors： Kasahara K,Shiina M,Higo J,Ogata K,Nakamura H

    更新日期：2018-03-16 00:00:00

  • TFIIH is an elongation factor of RNA polymerase I.

    abstract：:TFIIH is a multisubunit factor essential for transcription initiation and promoter escape of RNA polymerase II and for the opening of damaged DNA double strands in nucleotide excision repair (NER). In this study, we have analyzed at which step of the transcription cycle TFIIH is essential for transcription by RNA poly...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkr746

    authors： Assfalg R,Lebedev A,Gonzalez OG,Schelling A,Koch S,Iben S

    更新日期：2012-01-01 00:00:00

  • PHUSER (Primer Help for USER): a novel tool for USER fusion primer design.

    abstract：:Uracil-Specific Exision Reagent (USER) fusion is a recently developed technique that allows for assembly of multiple DNA fragments in a few simple steps. However, designing primers for USER fusion is both tedious and time consuming. Here, we present the Primer Help for USER (PHUSER) software, a novel tool for designin...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkr394

    authors： Olsen LR,Hansen NB,Bonde MT,Genee HJ,Holm DK,Carlsen S,Hansen BG,Patil KR,Mortensen UH,Wernersson R

    更新日期：2011-07-01 00:00:00

  • Regulatory factor-X binding to mutant HLA-DRA promoter sequences.

    abstract：:The class II genes of the major histocompatibility complex (MHC) encode a family of cell surface glycoproteins that present processed antigen to the T cell receptor. Class II genes are regulated coordinately, responding to both immunologic and developmental signals. Conserved sequence elements 5' to class II genes hav...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/19.6.1243

    authors： Hasegawa SL,Sloan JH,Reith W,Mach B,Boss JM

    更新日期：1991-03-25 00:00:00

  • BioModels-15 years of sharing computational models in life science.

    abstract：:Computational modelling has become increasingly common in life science research. To provide a platform to support universal sharing, easy accessibility and model reproducibility, BioModels (https://www.ebi.ac.uk/biomodels/), a repository for mathematical models, was established in 2005. The current BioModels platform ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkz1055

    authors： Malik-Sheriff RS,Glont M,Nguyen TVN,Tiwari K,Roberts MG,Xavier A,Vu MT,Men J,Maire M,Kananathan S,Fairbanks EL,Meyer JP,Arankalle C,Varusai TM,Knight-Schrijver V,Li L,Dueñas-Roca C,Dass G,Keating SM,Park YM,Buso N

    更新日期：2020-01-08 00:00:00

  • Phosphorylation of PNKP by ATM prevents its proteasomal degradation and enhances resistance to oxidative stress.

    abstract：:We examined the mechanism regulating the cellular levels of PNKP, the major kinase/phosphatase involved in the repair of oxidative DNA damage, and find that it is controlled by ATM phosphorylation and ubiquitylation-dependent proteasomal degradation. We discovered that ATM-dependent phosphorylation of PNKP at serines ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks909

    authors： Parsons JL,Khoronenkova SV,Dianova II,Ternette N,Kessler BM,Datta PK,Dianov GL

    更新日期：2012-12-01 00:00:00

  • PPT-DB: the protein property prediction and testing database.

    abstract：:The protein property prediction and testing database (PPT-DB) is a database housing nearly 30 carefully curated databases, each of which contains commonly predicted protein property information. These properties include both structural (i.e. secondary structure, contact order, disulfide pairing) and dynamic (i.e. orde...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm800

    authors： Wishart DS,Arndt D,Berjanskii M,Guo AC,Shi Y,Shrivastava S,Zhou J,Zhou Y,Lin G

    更新日期：2008-01-01 00:00:00

  • BiSearch: primer-design and search tool for PCR on bisulfite-treated genomes.

    abstract：:Bisulfite genomic sequencing is the most widely used technique to analyze the 5-methylation of cytosines, the prevalent covalent DNA modification in mammals. The process is based on the selective transformation of unmethylated cytosines to uridines. Then, the investigated genomic regions are PCR amplified, subcloned a...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gni012

    authors： Tusnády GE,Simon I,Váradi A,Arányi T

    更新日期：2005-01-13 00:00:00

  • Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan.

    abstract：:The topoisomerase I (TOP1) inhibitor irinotecan triggers cell death by trapping TOP1 on DNA, generating cytotoxic protein-linked DNA breaks (PDBs). Despite its wide application in a variety of solid tumors, the mechanisms of cancer cell resistance to irinotecan remains poorly understood. Here, we generated colorectal ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw1026

    authors： Meisenberg C,Ashour ME,El-Shafie L,Liao C,Hodgson A,Pilborough A,Khurram SA,Downs JA,Ward SE,El-Khamisy SF

    更新日期：2017-02-17 00:00:00

  • The Mouse Genome Database (MGD): from genes to mice--a community resource for mouse biology.

    abstract：:The Mouse Genome Database (MGD) forms the core of the Mouse Genome Informatics (MGI) system (http://www.informatics.jax.org), a model organism database resource for the laboratory mouse. MGD provides essential integration of experimental knowledge for the mouse system with information annotated from both literature an...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gki113

    authors： Eppig JT,Bult CJ,Kadin JA,Richardson JE,Blake JA,Anagnostopoulos A,Baldarelli RM,Baya M,Beal JS,Bello SM,Boddy WJ,Bradt DW,Burkart DL,Butler NE,Campbell J,Cassell MA,Corbani LE,Cousins SL,Dahmen DJ,Dene H,Diehl AD

    更新日期：2005-01-01 00:00:00

  • Unusual structural features of the 5S ribosomal RNA from Streptococcus cremoris.

    abstract：:The nucleotide sequence of the 5S ribosomal RNA of Streptococcus cremoris has been determined. The sequence is 5' (sequence in text) 3'. Comparison of the S. cremoris 5S RNA sequence to an updated prokaryotic generalized 5S RNA structural model shows that this 5S RNA contains some unusual structural features. These fe...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/11.21.7569

    authors： Neimark H,Andersen J,Delihas N

    更新日期：1983-11-11 00:00:00

  • DNA multiplex hybridization on microarrays and thermodynamic stability in solution: a direct comparison.

    abstract：:Hybridization intensities of 30 distinct short duplex DNAs measured on spotted microarrays, were directly compared with thermodynamic stabilities measured in solution. DNA sequences were designed to promote formation of perfect match, or hybrid duplexes containing tandem mismatches. Thermodynamic parameters DeltaH deg...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm865

    authors： Fish DJ,Horne MT,Brewood GP,Goodarzi JP,Alemayehu S,Bhandiwad A,Searles RP,Benight AS

    更新日期：2007-01-01 00:00:00

  • Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs.

    abstract：:In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi-...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/29.16.3327

    authors： Hennecke M,Kwissa M,Metzger K,Oumard A,Kröger A,Schirmbeck R,Reimann J,Hauser H

    更新日期：2001-08-15 00:00:00

  • Gene F of plasmid RSF1010 codes for a low-molecular-weight repressor protein that autoregulates expression of the repAC operon.

    abstract：:The repAC operon of plasmid RSF1010 consists of the genes for proteins E, F, RepA (DNA helicase), and RepC (origin-binding initiator protein) and is transcriptionally initiated by a promoter called P4. We have studied the expression of the repAC operon in vivo by using fusions to the lacZ reporter gene. The results sh...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/18.21.6215

    authors： Maeser S,Scholz P,Otto S,Scherzinger E

    更新日期：1990-11-11 00:00:00

  • The interaction of high mobility proteins HMG14 and 17 with nucleosomes.

    abstract：:The interaction of the high mobility group proteins, HMG14 and HMG17, with nucleosome core particles has been studied. The results show that two molecules of HMG14/17 can be bound tightly but reversibly to each core particle and that their affinity for core particles is greater than their affinity for histone-free DNA...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/8.17.3757

    authors： Sandeen G,Wood WI,Felsenfeld G

    更新日期：1980-09-11 00:00:00

  • M-Coffee: combining multiple sequence alignment methods with T-Coffee.

    abstract：:We introduce M-Coffee, a meta-method for assembling multiple sequence alignments (MSA) by combining the output of several individual methods into one single MSA. M-Coffee is an extension of T-Coffee and uses consistency to estimate a consensus alignment. We show that the procedure is robust to variations in the choice...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkl091

    authors： Wallace IM,O'Sullivan O,Higgins DG,Notredame C

    更新日期：2006-03-23 00:00:00

  • Discovery of functional genomic motifs in viruses with ViReMa-a Virus Recombination Mapper-for analysis of next-generation sequencing data.

    abstract：:We developed an algorithm named ViReMa (Viral-Recombination-Mapper) to provide a versatile platform for rapid, sensitive and nucleotide-resolution detection of recombination junctions in viral genomes using next-generation sequencing data. Rather than mapping read segments of pre-defined lengths and positions, ViReMa ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt916

    authors： Routh A,Johnson JE

    更新日期：2014-01-01 00:00:00

  • R3D-2-MSA: the RNA 3D structure-to-multiple sequence alignment server.

    abstract：:The RNA 3D Structure-to-Multiple Sequence Alignment Server (R3D-2-MSA) is a new web service that seamlessly links RNA three-dimensional (3D) structures to high-quality RNA multiple sequence alignments (MSAs) from diverse biological sources. In this first release, R3D-2-MSA provides manual and programmatic access to cu...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv543

    authors： Cannone JJ,Sweeney BA,Petrov AI,Gutell RR,Zirbel CL,Leontis N

    更新日期：2015-07-01 00:00:00

  • Sequences of human immunoglobulin switch regions: implications for recombination and transcription.

    abstract：:We have sequenced the entire human S mu and S gamma 4 immunoglobulin heavy chain class switch regions, and have also completed the sequence of human S epsilon. S mu is composed predominantly of GAGCT and GGGCT pentameric repeats, with these units also being found in S epsilon at a much lower density. S mu-S gamma 4 ma...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/18.24.7305

    authors： Mills FC,Brooker JS,Camerini-Otero RD

    更新日期：1990-12-25 00:00:00

  • Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase.

    abstract：:The original cDNA sequence reported for the murine DNA methyltransferase (MTase) was not full length. Recently, additional cDNA sequences have been reported that lie upstream of the original and contain an extended open reading frame with three additional ATGs in frame with the coding region [Tucker et al . (1996) Pro...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/25.22.4666

    authors： Pradhan S,Talbot D,Sha M,Benner J,Hornstra L,Li E,Jaenisch R,Roberts RJ

    更新日期：1997-11-15 00:00:00

  • A group II intron in the Neurospora mitochondrial coI gene: nucleotide sequence and implications for splicing and molecular evolution.

    abstract：:The temperature-sensitive Neurospora nuclear mutant cyt18-1 is deficient in splicing many Group I mitochondrial introns when grown at its non-permissive temperature; however, splicing of intron 1 in the coI gene of the Adiopodoume (formerly called North Africa) strain is unaffected (R.A. Collins and A.M. Lambowitz, J....

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/17.22.9087

    authors： Field DJ,Sommerfield A,Saville BJ,Collins RA

    更新日期：1989-11-25 00:00:00

  • Atlas of Genetics and Cytogenetics in Oncology and Haematology, year 2003.

    abstract：:The 'Atlas of Genetics and Cytogenetics in Oncology and Haematology' (http://www.infobiogen.fr/services/chromcancer) contains concise and updated cards on genes involved in cancer, cytogenetics and clinical entities in oncology, and cancer-prone diseases, a portal towards genetics/cancer, and teaching materials in gen...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg126

    authors： Huret JL,Dessen P,Bernheim A

    更新日期：2003-01-01 00:00:00

  • Loss of EZH2 results in precocious mammary gland development and activation of STAT5-dependent genes.

    abstract：:Establishment and differentiation of mammary alveoli during pregnancy are controlled by prolactin through the transcription factors STAT5A and STAT5B (STAT5), which also regulate temporal activation of mammary signature genes. This study addressed the question whether the methyltransferase and transcriptional co-activ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv776

    authors： Yoo KH,Oh S,Kang K,Hensel T,Robinson GW,Hennighausen L

    更新日期：2015-10-15 00:00:00