Abstract：
:Post-transcriptional modifications are characteristic features of tRNAs and have been shown in a number of cases to influence both their structural and functional properties, including structure stabilization, amino-acylation and codon recognition. We have developed an approach which allows the investigation of the post-transcriptional modification patterns of human mitochondrial wild-type and mutant tRNAs at both the qualitative and the quantitative levels. Specific tRNA species are long-term labeled in vivo with [32P]orthophosphate, isolated in a highly selective way, enzymatically digested to mononucleotides and then subjected to two-dimensional thin layer chromatographic analysis. The wild-type tRNALysand the corresponding tRNALyscarrying the A8344G mutation associated with the MERRF (Myoclonic Epilepsy with Ragged Red Fibers) syndrome exhibit the same modified nucleotides at the same molar concentrations. By contrast, a quantitatively different modification pattern was observed between the wild-type tRNALeu(UUR)and its counterpart carrying the A3243G mutation associated with the MELAS (Mitochondrial Myopathy, Encephalopathy with Lactic Acidosis and Stroke-like episodes) syndrome, the latter exhibiting a 50% decrease in m2G content. Complementary sequencing of tRNALeu(UUR)has allowed the localization of this modification at position 10 within the D-stem of the tRNA. The decreased level of this modification may have important implications for understanding the molecular mechanism underlying the MELAS-associated mitochondrial dysfunction.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Helm M,Florentz C,Chomyn A,Attardi Gdoi
10.1093/nar/27.3.756subject
Has Abstractpub_date
1999-02-01 00:00:00pages
756-63issue
3eissn
0305-1048issn
1362-4962pii
gkc161journal_volume
27pub_type
杂志文章abstract：:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...
journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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更新日期：1999-02-01 00:00:00
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更新日期：1996-07-15 00:00:00
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journal_title：Nucleic acids research
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更新日期：1992-04-11 00:00:00
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更新日期：2004-11-16 00:00:00
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更新日期：2002-01-01 00:00:00
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更新日期：2009-08-01 00:00:00
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更新日期：2006-01-31 00:00:00
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更新日期：1989-12-25 00:00:00
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更新日期：2014-01-01 00:00:00
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更新日期：2017-01-04 00:00:00
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更新日期：1984-11-26 00:00:00
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更新日期：2002-06-01 00:00:00
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更新日期：2021-01-25 00:00:00
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更新日期：2000-03-15 00:00:00
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更新日期：2011-02-01 00:00:00
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更新日期：1980-04-25 00:00:00
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更新日期：2009-11-01 00:00:00
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