Abstract：
:Among a collection of 74 aprt mutations induced by treatment of plateau phase Chinese hamster ovary CHO cells with the radiomimetic DNA double-strand cleaving agent neocarzinostatin, nine were large-scale rearrangements. Molecular analysis indicated that all nine were highly conservative, non-homologous reciprocal exchanges, most of which were intrachromosomal as determined by fluorescence in situ hybridization. All but one of the parental sequences contained potential double-strand cleavage sites positioned such that the observed rearrangements could be explained by drug-induced double-strand breakage followed by trimming, templated patching and ligation of the exchanged ends. Predicted non-complementary 3' overhangs were often preserved in the newly formed junctions, suggesting alignment-based fill-in of the overhangs. Banding of metaphase spreads of these mutants, and of a number of mutants induced by the functionally similar compound bleomycin, revealed that bleomycin-induced reciprocal exchange mutants had multiple additional chromosome alterations and considerable chromosomal heterogeneity within each mutant line. In contrast, neocarzinostatin-induced reciprocal exchange mutants, as well as bleomycin-induced base substitution and single base deletion mutants, retained stable pseudodiploid karyotypes similar to that of the parent line. Thus, some reciprocal exchanges arising from misjoining of double-strand breaks were associated with global chromosomal instability, while other ostensibly similar events were not.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Wang P,Lee JW,Yu Y,Turner K,Zou Y,Jackson-Cook CK,Povirk LFdoi
10.1093/nar/gkf369keywords:
subject
Has Abstractpub_date
2002-06-15 00:00:00pages
2639-46issue
12eissn
0305-1048issn
1362-4962journal_volume
30pub_type
杂志文章abstract：:In order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. A 16-mer oligonucleotide (ODN) was derivatized at the 5' prime through a 32P phosphate spacer with cholesterol, yielding a 32P-labeled amphiphatic cho...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.17.4695
更新日期：1991-09-11 00:00:00
abstract：:Embryonic stem cells (ESCs) and meiosis are featured by relatively higher frequent homologous recombination associated with DNA double strand breaks (DSB) repair. Here, we show that Pold3 plays important roles in DSB repair, telomere maintenance and genomic stability of both ESCs and spermatocytes in mice. By attempti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky098
更新日期：2018-04-20 00:00:00
abstract：:We have investigated the mechanism of the electrophoresis-driven chromatin aggregation which had been described by Weintraub (1984, Cell 38, 17-27) as a putative mean for propagation of genetic repression in eukaryotes. We show that the oligonucleosome aggregates are assembled de novo at the starting zone of DNP elect...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.24.7397
更新日期：1990-12-25 00:00:00
abstract：:With discovery of diverse roles for RNA, its centrality in cellular functions has become increasingly apparent. A number of algorithms have been developed to predict RNA secondary structure. Their performance has been benchmarked by comparing structure predictions to reference secondary structures. Generally, algorith...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1081
更新日期：2012-02-01 00:00:00
abstract：:Molecular Biology Database List (MBDL) includes brief descriptions and pointers to Web sites for the various databases described in this issue as well as other Web sites presenting data sets relevant to molecular biology. This information is compiled into a list (http://www.oup.co.uk/nar/Volume_27/Issue_01/summary/ gk...
journal_title：Nucleic acids research
pub_type：
doi：10.1093/nar/27.1.1
更新日期：1999-01-01 00:00:00
abstract：:DNA assembly forms the cornerstone of modern synthetic biology. Despite the numerous available methods, scarless multi-fragment assembly of large plasmids remains challenging. Furthermore, the upcoming wave in molecular biological automation demands a rethinking of how we perform DNA assembly. To streamline automation...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx132
更新日期：2017-06-20 00:00:00
abstract：:We have developed an end-labelling approach to map the positions of nucleosomes and protein binding sites at nucleotide resolution by footprinting micrococcal nuclease (MNase)-sensitive sites. Using this approach we determined that the MFA2 gene and its upstream control regions have four positioned nucleosomes when tr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.13.e64
更新日期：2001-07-01 00:00:00
abstract：:Chicken erythrocyte inner histones (H2A, H2B, H3 and H4) were associated with the two complementary homopolymeric polydeoxyribonucleotides and the two alternating copolymeric polydeoxyribonucleotides. No evidence for formation of chromatin-like structures was obtained for the complexes with poly(dG) . poly(dC) or poly...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.4.1387
更新日期：1979-04-01 00:00:00
abstract：:The binding kinetics of NF-kappaB p50 to the Ig-kappaB site and to a DNA duplex with no specific binding site were determined under varying conditions of potassium chloride concentration using a surface plasmonresonance biosensor. Association and dissociation rate constants were measured enabling calculation of the di...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.4.1063
更新日期：1999-02-15 00:00:00
abstract：:The oncogenic transformation of normal cells into malignant, rapidly proliferating cells requires major alterations in cell physiology. For example, the transformed cells remodel their metabolic processes to supply the additional demand for cellular building blocks. We have recently demonstrated essential metabolic pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1337
更新日期：2016-01-04 00:00:00
abstract：:The nucleotide sequence of a 2.14 kb fragment of Bacillus subtilis DNA containing the citG gene encoding fumarase was determined using the dideoxy chain termination method. The citG coding region of 1392 base pairs (464 codons) was identified, and the deduced Mr (50425) is in good agreement with that of the protein id...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.1.131
更新日期：1985-01-11 00:00:00
abstract：:The results of differential expression analyses provide scientists with hundreds to thousands of differentially expressed genes that need to be interpreted in light of the biology of the specific system under study. This requires mapping the genes to functional classifications that can be, for example, the KEGG pathwa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt516
更新日期：2013-07-01 00:00:00
abstract：:Reversion of T type cytoplasmic male sterility (cmsT) to fertility is correlated with sequence changes in a 1.5 kb AvaI fragment. This 1.5 kb AvaI fragment is composed of 5' flanking sequences of ATPase subunit 6, one complete open reading frame (ORF 13) and part of the another (ORF 25). The sequence of the 1.5 kb Ava...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.15.6091
更新日期：1987-08-11 00:00:00
abstract：:Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.3.915
更新日期：1979-03-01 00:00:00
abstract：:One-Block CYRCA is an automated procedure for identifying multiple-block alignments from single block queries (http://bioinfo.weizmann.ac.il/blocks/OneCYRCA). It is based on the LAMA and CYRCA block-to-block alignment methods. The procedure identifies whether the query blocks can form new multiple-block alignments (bl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki488
更新日期：2005-07-01 00:00:00
abstract：:Bulk replicative DNA synthesis in eukaryotes is highly accurate and efficient, primarily because of two DNA polymerases (Pols): Pols δ and ε. The high fidelity of these enzymes is due to their intrinsic base selectivity and proofreading exonuclease activity which, when coupled with post-replication mismatch repair, he...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1034
更新日期：2011-03-01 00:00:00
abstract：:The functionalisation of oligodeoxynucleotides and oligoribonucleotides by incorporation of 2'-amido-2'-deoxyribonucleosides, possibly containing a reporter group via the 2'-amido bond, was examined. Therefore 2'-acetamido-ribonucleosides containing a small methyl group at the 2'-amido bond were synthesized as model c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.1.51
更新日期：1995-01-11 00:00:00
abstract：:In Escherichia coli, the MnmEG complex modifies transfer RNAs (tRNAs) decoding NNA/NNG codons. MnmEG catalyzes two different modification reactions, which add an aminomethyl (nm) or carboxymethylaminomethyl (cmnm) group to position 5 of the anticodon wobble uridine using ammonium or glycine, respectively. In tRNA(cmnm...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1228
更新日期：2014-02-01 00:00:00
abstract：:A number of cysteine-rich proteins have recently been isolated by homology screening, differential library screens, and association with other proteins. In this report, we describe the isolation of the rat cysteine-rich protein from a rat brain library during a search for clones with homology to the delta-opioid recep...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.24.5477
更新日期：1994-12-11 00:00:00
abstract：:Soil salinity is a significant threat to sustainable agricultural production worldwide. Plants must adjust their developmental and physiological processes to cope with salt stress. Although the capacity for adaptation ultimately depends on the genome, the exceptional versatility in gene regulation provided by the spli...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1229
更新日期：2018-02-28 00:00:00
abstract：:We have constructed deletion mutants of simian virus 40 (SV40) lacking the two tandemly repeated copies or all three copies of the 21-bp repeated sequence located in the origin region. The mutants were viable, but had lower infectivities compared to the wild type. The mutant lacking two copies of the 21-bp repeat grew...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.5.1601
更新日期：1983-03-11 00:00:00
abstract：:DNA polymerase gamma from purified nuclei of EMT-6 cells (mice) seems to be identical to the mitochondrial DNA polymerase from the same source following several criteria. These two enzyme activities are strongly inhibited by ethidium bromide and acriflavin, while proflavin, acridine orange, daunomycin and chloroquine ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.6.2197
更新日期：1978-06-01 00:00:00
abstract：:Human telomerase is a ribonucleoprotein which uses its internal RNA moiety as a template for telomeric DNA synthesis. This enzyme is up-regulated in most malignant tumors and is therefore considered as a possible cancer target. Here we examined the effects of differently modified oligomers on telomeraseactivity from H...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.4.1152
更新日期：1999-02-15 00:00:00
abstract：:A previously elaborated technique for fixing a chosen partially melted state of DNA with glyoxal was used in a study of the melting process of the replicative form (RF III) of phi X174 DNA. Electron-microscopic maps corresponding to five points of the melting curve of RF III were obtained and compared with the theoret...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.6.1867
更新日期：1982-03-25 00:00:00
abstract：:Many RNP proteins contain one or more copies of the RNA recognition motif (RRM) and are thought to be involved in cellular RNA metabolism. We have previously characterized in Xenopus a nervous system specific gene, nrp1, that is more similar to the hnRNP A/B proteins than to other known proteins (K. Richter, P. J. Goo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.4.999
更新日期：1993-02-25 00:00:00
abstract：:We describe PReMod, a new database of genome-wide cis-regulatory module (CRM) predictions for both the human and the mouse genomes. The prediction algorithm, described previously in Blanchette et al. (2006) Genome Res., 16, 656-668, exploits the fact that many known CRMs are made of clusters of phylogenetically conser...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl879
更新日期：2007-01-01 00:00:00
abstract：:DNA synthesis by phage T4 DNA polymerase is arrested at specific sequences in single-stranded DNA templates. To determine whether or not T4 DNA polymerase accessory proteins 32, 44, 45 and 62 eliminated recognition of these arrest sites, unique primer-templates were constructed in which DNA synthesis began at a DNA pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.8.3343
更新日期：1986-04-25 00:00:00
abstract：:We have isolated and characterized a S. pombe promoter using a functional heterologous gene product assay. Random S. pombe genomic fragments were cloned upstream from the promoterless 'lacZ gene and tested in vivo for their efficiency to promote expression of the beta-galactosidase protein in the fission yeast. An eff...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.17.8603
更新日期：1988-09-12 00:00:00
abstract：:We have developed a web server, PRince, which analyzes the structural features and physicochemical properties of the protein-RNA interface. Users need to submit a PDB file containing the atomic coordinates of both the protein and the RNA molecules in complex form (in '.pdb' format). They should also mention the chain ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks535
更新日期：2012-07-01 00:00:00
abstract：:Small RNA (sRNA) Sequencing technology has revealed that microRNAs (miRNAs) are capable of exhibiting frequent variations from their canonical sequences, generating multiple variants: the isoforms of miRNAs (isomiRs). However, integrated tool to precisely detect and systematically annotate isomiRs from sRNA sequencing...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw427
更新日期：2016-07-08 00:00:00
