Abstract：
:We have constructed deletion mutants of simian virus 40 (SV40) lacking the two tandemly repeated copies or all three copies of the 21-bp repeated sequence located in the origin region. The mutants were viable, but had lower infectivities compared to the wild type. The mutant lacking two copies of the 21-bp repeat grew fairly well indicating that the one copy of the 21-bp repeat it contains is adequate. The other mutant lacking all the three copies of the 21-bp repeat was also viable but grew poorly. The viability of this mutant suggests that the upstream 72-bp repeated sequence compensates, though only partially, for the absence of the 21-bp repeat. The growth deficiencies of the deletion mutants could not be overcome by complementation with temperature-sensitive helper mutants providing either the early or the late functions of the virus, suggesting that the deficiencies lie in both early and late gene expression and/or in replication.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hartzell SW,Yamaguchi J,Subramanian KNdoi
10.1093/nar/11.5.1601subject
Has Abstractpub_date
1983-03-11 00:00:00pages
1601-16issue
5eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract：:Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain dir...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.12.4921
更新日期：1984-06-25 00:00:00
abstract：:The conformational changes induced by the binding of cis-diamminedichloroplatinum(II) to poly(dG-dC).poly(dG-dC) have been studied by reaction with specific antibodies, by circular dichroism and 31P nuclear magnetic resonance. Polyclonal and monoclonal antibodies to Z-DNA bind to platinated poly(dG-dC).poly(dG-dC) at ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.14.5767
更新日期：1984-07-25 00:00:00
abstract：:A number of meiosis-specific transcripts are selectively eliminated during the mitotic cell cycle in fission yeast. Mmi1, an RNA-binding protein, plays a crucial role in this selective elimination. Mmi1 recognizes a specific region, namely, the determinant of selective removal (DSR) on meiotic transcripts and induces ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt763
更新日期：2013-11-01 00:00:00
abstract：:The Protein Folding Database (PFD) is a publicly accessible repository of thermodynamic and kinetic protein folding data. Here we describe the first major revision of this work, featuring extensive restructuring that conforms to standards set out by the recently formed International Foldeomics Consortium. The database...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl1007
更新日期：2007-01-01 00:00:00
abstract：:Clustered regularly interspaced short palindromic repeats (CRISPR), in combination with CRISPR associated (cas) genes, constitute CRISPR-Cas bacterial adaptive immune systems. To generate immunity, these systems acquire short sequences of nucleic acids from foreign invaders and incorporate these into their CRISPR arra...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku527
更新日期：2014-07-01 00:00:00
abstract：:A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.1.19
更新日期：1981-01-10 00:00:00
abstract：:We have developed an HIV nef-Escherichia coli lacZ fusion system in vitro that allows the detection of low frequency mutations, including frameshifts, deletions and insertions. A portion of the nef gene that encompasses a hypervariable region was fused in-frame with a downstream lacZalpha peptide coding region. The re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.9.1710
更新日期：1996-05-01 00:00:00
abstract：:According to recent models, as yet poorly studied architectural proteins appear to be required for local regulation of enhancer-promoter interactions, as well as for global chromosome organization. Transcription factors ZIPIC, Pita and Zw5 belong to the class of chromatin insulator proteins and preferentially bind to ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw371
更新日期：2016-09-06 00:00:00
abstract：:LINE1s occupy 17% of the human genome and are its only active autonomous mobile DNA. L1s are also responsible for genomic insertion of processed pseudogenes and >1 million non-autonomous retrotransposons (Alus and SVAs). These elements have significant effects on gene organization and expression. Despite the importanc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt512
更新日期：2013-08-01 00:00:00
abstract：:Enzymatic hydrolysis and base analysis by high performance liquid chromatography showed that mouse satellite DNA had 30-50% less 5-methylcytosine in sperm than in somatic tissue (1.59 mols % vs 2.40-3.11 mols %). Maxam-Gilbert sequencing and analysis of the intensity of the cytosine bands indicated that the level of m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.11.3969
更新日期：1985-06-11 00:00:00
abstract：:Genetic recombination in meiosis plays an important role in generating diversity of genetic information. In yeast an Escherichia coli RecA-like gene, DMC1, is expressed in meiotic prophase and its product co-localizes with Rad51 protein on zygotene chromosomes. We have cloned the mouse and human homologs of the yeast ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.3.470
更新日期：1996-02-01 00:00:00
abstract：:Communication between distantly spaced genomic regions is one of the key features of gene regulation in eukaryotes. Chromatin per se can stimulate efficient enhancer-promoter communication (EPC); however, the role of chromatin structure and dynamics in this process remains poorly understood. Here we show that nucleoso...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1240
更新日期：2017-04-07 00:00:00
abstract：:MiR-210 is up-regulated in multiple cancer types but its function is disputable and further investigation is necessary. Using a bioinformatics approach, we identified the putative target genes of miR-210 in hypoxia-induced CNE cells from genome-wide scale. Two functional gene groups related to cell cycle and RNA proce...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks995
更新日期：2013-01-07 00:00:00
abstract：:An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its applicat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.9.2963
更新日期：1979-07-11 00:00:00
abstract：:G-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw011
更新日期：2016-05-19 00:00:00
abstract：:A human telomere ends in a single-stranded 3' tail, composed of repeats of T2AG3. G-quadruplexes (GQs) formed from four consecutive repeats have been shown to possess high-structural and mechanical diversity. In principle, a GQ can form from any four repeats that are not necessarily consecutive. To understand the dyna...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz906
更新日期：2019-12-02 00:00:00
abstract：:The NCBI Taxonomy database (http://www.ncbi.nlm.nih.gov/taxonomy) is the standard nomenclature and classification repository for the International Nucleotide Sequence Database Collaboration (INSDC), comprising the GenBank, ENA (EMBL) and DDBJ databases. It includes organism names and taxonomic lineages for each of the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1178
更新日期：2012-01-01 00:00:00
abstract：:The Escherichia coli AlkB protein (EcAlkB) is a DNA repair enzyme which reverses methylation damage such as 1-methyladenine (1-meA) and 3-methylcytosine (3-meC). The mammalian AlkB homologues ALKBH2 and ALKBH3 display EcAlkB-like repair activity in vitro, but their substrate specificities are different, and ALKBH2 is ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks327
更新日期：2012-08-01 00:00:00
abstract：:Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA seed regions (posi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq341
更新日期：2010-09-01 00:00:00
abstract：:We recently compared the efficiency of six picornaviral internal ribosome entry segments (IRESes) and the hepatitis C virus (HCV) IRES for their ability to drive internal initiation of translationin vitro. Here we present the results of a similar comparison performed in six different cultured cell lines infected with ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.5.925
更新日期：1997-03-01 00:00:00
abstract：:The mini-exon, a short segment found at the 5' end of trypanosome mRNAs, is contributed by a small RNA, the mini-exon donor (medRNA). In vivo 32P-labeled medRNA, a set of smaller RNAs related to it, and mRNA, were purified from Trypanosoma brucei by hybrid selection and gel electrophoresis. Using RNA fingerprinting an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.23.9861
更新日期：1987-12-10 00:00:00
abstract：:Affymetrix has recently developed whole-transcript GeneChips-'Gene' and 'Exon' arrays-which interrogate exons along the length of each gene. Although each probe on these arrays is intended to hybridize perfectly to only one transcriptional target, many probes match multiple transcripts located in different parts of th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp853
更新日期：2010-01-01 00:00:00
abstract：:The RNA degradosome is a multi-enzyme assembly that contributes to key processes of RNA metabolism, and it engages numerous partners in serving its varied functional roles. Small domains within the assembly recognize collectively a diverse range of macromolecules, including the core protein components, the cytoplasmic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks739
更新日期：2012-11-01 00:00:00
abstract：:The representational difference analysis (RDA) and other subtraction techniques are used to enrich sample-specific sequences by elimination of ubiquitous sequences existing in both the sample of interest (tester) and the subtraction partner (driver). While applying the RDA to genomic DNA of cutaneous lymphoma cells in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.4.e20
更新日期：2001-02-15 00:00:00
abstract：:In addition to the cap-dependent mechanism, eukaryotic initiation of translation can occur by a cap-independent mechanism which directs ribosomes to defined start codons enabled by internal ribosome entry site (IRES) elements. IRES elements from poliovirus and encephalomyocarditis virus are often used to construct bi-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.16.3327
更新日期：2001-08-15 00:00:00
abstract：:The human lymphoid cell lines Walker and Daudi constitute a particularly suitable system for studies on the chromatin structure of K light chain genes (see preceding paper). The rearranged and non-rearranged alleles of Walker cells were found to be about equally sensitive towards digestion with DNAase I. A DNAase I hy...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.18.7007
更新日期：1984-09-25 00:00:00
abstract：:Differential melting profiles and denaturation maps are calculated for fdDNA whose sequence of nucleotides has been determined recently. The melting profiles for the total DNA and a number of its restriction fragments are compared with experimental data taken from literature. The comparison enables one to correlate a ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.7.2547
更新日期：1978-07-01 00:00:00
abstract：:SplicePort is a web-based tool for splice-site analysis that allows the user to make splice-site predictions for submitted sequences. In addition, the user can also browse the rich catalog of features that underlies these predictions, and which we have found capable of providing high classification accuracy on human s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm407
更新日期：2007-07-01 00:00:00
abstract：:Current approaches to design efficient antisense RNAs (asRNAs) rely primarily on a thermodynamic understanding of RNA-RNA interactions. However, these approaches depend on structure predictions and have limited accuracy, arguably due to overlooking important cellular environment factors. In this work, we develop a bio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx115
更新日期：2017-05-19 00:00:00
abstract：:Peroxisomes are induced in Saccharomyces cerevisiae when this yeast is grown in the presence of oleate, and are repressed when glucose is supplied as the carbon source. Concomitant with this is an induction/repression of peroxisomal beta-oxidation enzymes. We are investigating the transcriptional control of acyl-CoA o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.13.3495
更新日期：1992-07-11 00:00:00
