Abstract:
:Differential melting profiles and denaturation maps are calculated for fdDNA whose sequence of nucleotides has been determined recently. The melting profiles for the total DNA and a number of its restriction fragments are compared with experimental data taken from literature. The comparison enables one to correlate a number of peaks on experimental melting profiles with the melting out of concrete regions of the nucleotide sequence. For three fragments very strong end effects are demonstrated on both theoretical and experimental profiles. These anomalous end effects are shown to be connected with a region highly enriched with AT-pairs. A possible influence of the heterogeneity of stacking interaction on the results obtained is discussed in detail.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Vologodskii AV,Frank-Kamenetskii MDdoi
10.1093/nar/5.7.2547subject
Has Abstractpub_date
1978-07-01 00:00:00pages
2547-56issue
7eissn
0305-1048issn
1362-4962journal_volume
5pub_type
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1993-05-25 00:00:00
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更新日期:1978-07-01 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2011-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-06-24 00:00:00
abstract:UNLABELLED:Partial cleavage of DNA with the restriction endonuclease NotI (5'...GC/GGCCGC...3') is an important technique for genomic mapping. However, partial genomic cleavage with this enzyme is impaired by the agarose matrix in which the DNA must be suspended. To solve this problem we have purified the blocking meth...
journal_title:Nucleic acids research
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pub_type: 杂志文章,评审
doi:10.1093/nar/29.18.3784
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1988-10-25 00:00:00
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journal_title:Nucleic acids research
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更新日期:2017-03-17 00:00:00