Detection of labelled RNA species by contact hybridization.

abstract：:To investigate the mechanisms by which oligonucleotides hybridize to target molecules, the binding of two oligodeoxynucleotide probes to RNA targets was measured over a broad range of temperatures. Mutations were then scanned across each DNA/RNA hybrid to map, at single base resolution, sequences important for hybridi...

journal_title：Nucleic acids research

authors： Majlessi M,Becker MM

更新日期：2008-05-01 00:00:00

abstract：:The field bean, Vicia faba L. var. minor, possesses two sub-families of 11 S legumin genes named A and B. We isolated from a genomic library a B-type gene (LeB4) and determined its primary DNA sequence. Gene LeB4 codes for a 484 amino acid residue prepropolypeptide, encompassing a signal peptide of 22 amino acid resid...

journal_title：Nucleic acids research

authors： Bäumlein H,Wobus U,Pustell J,Kafatos FC

更新日期：1986-03-25 00:00:00

abstract：:Next-generation sequencing allows now the sequencing of small RNA molecules and the estimation of their expression levels. Consequently, there will be a high demand of bioinformatics tools to cope with the several gigabytes of sequence data generated in each single deep-sequencing experiment. Given this scene, we deve...

journal_title：Nucleic acids research

authors： Hackenberg M,Sturm M,Langenberger D,Falcón-Pérez JM,Aransay AM

更新日期：2009-07-01 00:00:00

abstract：:About 40% of the genes in the nematode Caenorhabditis elegans have homologs in humans. Based on the history of this model system, it is clear that the application of genetic methods to the study of this set of genes would provide important clues to their function in humans. To facilitate such genetic studies, we are e...

journal_title：Nucleic acids research

authors： Edgley M,D'Souza A,Moulder G,McKay S,Shen B,Gilchrist E,Moerman D,Barstead R

更新日期：2002-06-15 00:00:00

abstract：:Toscana virus (TOSV) is an arthropod-borne human pathogen responsible for seasonal outbreaks of fever and meningoencephalitis in the Mediterranean basin. TOSV is a segmented negative-strand RNA virus (sNSV) that belongs to the genus phlebovirus (family Phenuiviridae, order Bunyavirales), encompassing other important h...

journal_title：Nucleic acids research

authors： Jones R,Lessoued S,Meier K,Devignot S,Barata-García S,Mate M,Bragagnolo G,Weber F,Rosenthal M,Reguera J

更新日期：2019-11-18 00:00:00

abstract：:The inherent properties of DNA as a stable polymer with unique affinity for partner molecules determined by the specific Watson-Crick base pairing makes it an ideal component in self-assembling structures. This has been exploited for decades in the design of a variety of artificial substrates for investigations of DNA...

journal_title：Nucleic acids research

authors： Andersen FF,Knudsen B,Oliveira CL,Frøhlich RF,Krüger D,Bungert J,Agbandje-McKenna M,McKenna R,Juul S,Veigaard C,Koch J,Rubinstein JL,Guldbrandtsen B,Hede MS,Karlsson G,Andersen AH,Pedersen JS,Knudsen BR

更新日期：2008-03-01 00:00:00

abstract：:Antisense oligodeoxynucleotides targeted to Ha-ras mRNA have been designed to discriminate between the codon 12-mutated oncogene and the normal proto-oncogene. An in vitro assay using two different sources of RNase H (rabbit reticulocyte lysates and nuclear extract from HeLa cells) was used to characterize oligonucleo...

journal_title：Nucleic acids research

authors： Duroux I,Godard G,Boidot-Forget M,Schwab G,Hélène C,Saison-Behmoaras T

更新日期：1995-09-11 00:00:00

abstract：:Guanine-quadruplexes (G4) consist of non-canonical four-stranded helical arrangements of guanine-rich nucleic acid sequences. The bulky and thermodynamically stable features of G4 structures have been shown in many respects to affect normal nucleic acid metabolism. In vivo conversion of G4 structures to single-strande...

journal_title：Nucleic acids research

authors： Lattmann S,Stadler MB,Vaughn JP,Akman SA,Nagamine Y

更新日期：2011-11-01 00:00:00

abstract：:Combinatorial interactions of sequence-specific trans-acting factors with localized genomic cis-element clusters are the principal mechanism for regulating tissue-specific and developmental gene expression. With the emergence of expanding numbers of genome-wide expression analyses, the identification of the cis-elemen...

journal_title：Nucleic acids research

authors： Jegga AG,Gupta A,Gowrisankar S,Deshmukh MA,Connolly S,Finley K,Aronow BJ

更新日期：2005-07-01 00:00:00

abstract：:In the process of establishing more and more complete annotations of eukaryotic genomes, a constantly growing number of alternative splicing (AS) events has been reported over the last decade. Consequently, the increasing transcript coverage also revealed the real complexity of some variations in the exon-intron struc...

journal_title：Nucleic acids research

authors： Foissac S,Sammeth M

更新日期：2007-07-01 00:00:00

abstract：:Genome editing using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) predominantly induces non-homologous end joining (NHEJ), which generates random insertions or deletions, whereas homology-directed repair (HDR), which generates precise recombination products, is ...

journal_title：Nucleic acids research

authors： Kato-Inui T,Takahashi G,Hsu S,Miyaoka Y

更新日期：2018-05-18 00:00:00

abstract：:A UCSD Pascal program was developed which can analyze nucleic acid dot matrices of up to 9500 X 9500 in size on the Apple II computer. Although matrices of such size consume large amounts of computer memory, this program minimizes these problems by analyzing only small strips of the matrix at a time, and then transfer...

journal_title：Nucleic acids research

authors： Zweig SE

更新日期：1984-01-11 00:00:00

abstract：:An attempt has been made to correlate differential scanning calorimetry melting profiles of 5S rRNAs from lupin seeds (L.s.) and wheat germ (W.g.) with their structure. It is suggested that the observed differences in thermal unfolding are due to differences in RNA nucleotide sequence and as a consequence in higher or...

journal_title：Nucleic acids research

authors： Barciszewski J,Bratek-Wiewiórowska MD,Górnicki P,Naskret-Barciszewska M,Wiewiórowski M,Zielenkiewicz A,Zielenkiewicz W

更新日期：1988-01-25 00:00:00

abstract：:We have constructed simple test-plasmids to study transcriptional enhancers in yeast. In this paper the reporter-gene is a plasmid borne deletion-substitution derivative (his-del4) of the Saccharomyces cerevisiae HIS3 gene in which the native promoter has been replaced by a dormant, susceptible pseudopromoter. We inve...

journal_title：Nucleic acids research

authors： Ciaramella M,Sacco M,Pulitzer JF

更新日期：1988-09-26 00:00:00

abstract：:Short oligo(deoxynucleoside phosphorothioate)s were analyzed as a pool of individual diastereomeric species. The composition of such mixtures, determined by means of HPLC, indicates that consecutive couplings in commonly used phosphoramidite chemistry lead to increasing contents of the Rp isomer. Methods of analysis a...

journal_title：Nucleic acids research

authors： Wilk A,Stec WJ

更新日期：1995-02-11 00:00:00

abstract：:A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtaine...

journal_title：Nucleic acids research

authors： Demidov V,Frank-Kamenetskii MD,Egholm M,Buchardt O,Nielsen PE

更新日期：1993-05-11 00:00:00

abstract：:Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, ...

journal_title：Nucleic acids research

authors： Sinclair JH,Bryant LA

更新日期：1987-11-25 00:00:00

abstract：:The mammalian target of rapamycin (mTOR) is a critical regulator of cell growth, integrating multiple signalling cues and pathways. Key among the downstream activities of mTOR is the control of the protein synthesis machinery. This is achieved, in part, via the co-ordinated regulation of mRNAs that contain a terminal ...

journal_title：Nucleic acids research

authors： Smith EM,Benbahouche NEH,Morris K,Wilczynska A,Gillen S,Schmidt T,Meijer HA,Jukes-Jones R,Cain K,Jones C,Stoneley M,Waldron JA,Bell C,Fonseca BD,Blagden S,Willis AE,Bushell M

更新日期：2021-01-11 00:00:00

abstract：:Synchronized cells of a normal human lymphocytic cell line contain little swiven enzyme activity in G0 and G1 and high activity in Sphase. The level of activity in different growth phase appears to be related to the fraction of the population engaged in DNA replication. No endogenous inhibitor or activator of swiven a...

journal_title：Nucleic acids research

authors： Rosenberg BH,Ungers G,Deutsch JF

更新日期：1976-12-01 00:00:00

abstract：:Gramene (http://www.gramene.org) is a knowledgebase for comparative functional analysis in major crops and model plant species. The current release, #54, includes over 1.7 million genes from 44 reference genomes, most of which were organized into 62,367 gene families through orthologous and paralogous gene classificat...

journal_title：Nucleic acids research

authors： Tello-Ruiz MK,Naithani S,Stein JC,Gupta P,Campbell M,Olson A,Wei S,Preece J,Geniza MJ,Jiao Y,Lee YK,Wang B,Mulvaney J,Chougule K,Elser J,Al-Bader N,Kumari S,Thomason J,Kumar V,Bolser DM,Naamati G,Tapanari E,Fo

更新日期：2018-01-04 00:00:00

abstract：:Rap1 is an essential DNA-binding factor from the yeast Saccharomyces cerevisiae involved in transcription and telomere maintenance. Its binding to DNA targets Rap1 at particular loci, and may optimize its ability to form functional macromolecular assemblies. It is a modular protein, rich in large potentially unfolded ...

journal_title：Nucleic acids research

authors： Matot B,Le Bihan YV,Lescasse R,Pérez J,Miron S,David G,Castaing B,Weber P,Raynal B,Zinn-Justin S,Gasparini S,Le Du MH

更新日期：2012-04-01 00:00:00

abstract：:E2F transcription factors (E2F1-8) are known to coordinately regulate the expression of a plethora of target genes, including those coding for microRNAs (miRNAs), to control cell cycle progression. Recent work has described the atypical E2F factor E2F7 as a transcriptional repressor of cell cycle-related protein-codin...

journal_title：Nucleic acids research

authors： Mitxelena J,Apraiz A,Vallejo-Rodríguez J,Malumbres M,Zubiaga AM

更新日期：2016-07-08 00:00:00

abstract：:We have developed general methods for joining together, via cleavable disulfide bonds, either two unprotected polynucleotides or a polynucleotide and a peptide or protein. To join two oligonucleotides, each is first converted to an adduct in which cystamine is joined to the 5'-terminal phosphate of the oligonucleotide...

journal_title：Nucleic acids research

authors： Chu BC,Orgel LE

更新日期：1988-05-11 00:00:00

abstract：:Controller (C) proteins regulate the timing of the expression of restriction and modification (R-M) genes through a combination of positive and negative feedback circuits. A single dimer bound to the operator switches on transcription of the C-gene and the endonuclease gene; at higher concentrations, a second dimer bo...

journal_title：Nucleic acids research

authors： McGeehan JE,Streeter SD,Thresh SJ,Ball N,Ravelli RB,Kneale GG

更新日期：2008-08-01 00:00:00

abstract：:Elongation factor P (EF-P) is a conserved ribosome-binding protein that structurally mimics tRNA to enable the synthesis of peptides containing motifs that otherwise would induce translational stalling, including polyproline. In many bacteria, EF-P function requires post-translational modification with (R)-β-lysine by...

journal_title：Nucleic acids research

authors： Katz A,Solden L,Zou SB,Navarre WW,Ibba M

更新日期：2014-03-01 00:00:00

abstract：:An intronic hexanucleotide UGCAUG has been shown to play a critical role in the regulation of tissue-specific alternative splicing of pre-mRNAs in a wide range of tissues. Vertebrate Fox-1 has been shown to bind to this element, in a highly sequence-specific manner, through its RNA recognition motif (RRM). In mammals,...

journal_title：Nucleic acids research

authors： Nakahata S,Kawamoto S

更新日期：2005-04-11 00:00:00

abstract：:The immunoglobulin heavy chain intronic transcriptional enhancer (E mu) is part of a complex cis-regulatory DNA region which has notably been shown to modulate V(D)J rearrangements of associated variable gene segments. We have used recombination substrates comprised of the E mu enhancer together with various lengths o...

journal_title：Nucleic acids research

authors： Fernex C,Caillol D,Capone M,Krippl B,Ferrier P

更新日期：1994-03-11 00:00:00

abstract：:Peptidome similarity analysis enables researchers to gain insights into differential peptide profiles, providing a robust tool to discriminate strain-specific peptides, true intra-species differences among biological replicates or even microorganism-phenotype variations. However, no in silico peptide fingerprinting so...

journal_title：Nucleic acids research

authors： Blanco-Míguez A,Fdez-Riverola F,Lourenço A,Sánchez B

更新日期：2017-07-03 00:00:00

abstract：:RNA-binding proteins (RBPs) play important roles in the post-transcriptional control of RNAs. Identifying RBP binding sites and characterizing RBP binding preferences are key steps toward understanding the basic mechanisms of the post-transcriptional gene regulation. Though numerous computational methods have been dev...

journal_title：Nucleic acids research

authors： Zhang S,Zhou J,Hu H,Gong H,Chen L,Cheng C,Zeng J

更新日期：2016-02-29 00:00:00

abstract：:POGO-DB (http://pogo.ece.drexel.edu/) provides an easy platform for comparative microbial genomics. POGO-DB allows users to compare genomes using pre-computed metrics that were derived from extensive computationally intensive BLAST comparisons of >2000 microbes. These metrics include (i) average protein sequence ident...

journal_title：Nucleic acids research

authors： Lan Y,Morrison JC,Hershberg R,Rosen GL

更新日期：2014-01-01 00:00:00