Abstract:
:Short oligo(deoxynucleoside phosphorothioate)s were analyzed as a pool of individual diastereomeric species. The composition of such mixtures, determined by means of HPLC, indicates that consecutive couplings in commonly used phosphoramidite chemistry lead to increasing contents of the Rp isomer. Methods of analysis and mathematical basis for diastereomeric composition are discussed. Data presented include all 16 possible combinations of nucleosides in dinucleotide phosphorothioates, as well as examples of trimers and tetramers.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Wilk A,Stec WJdoi
10.1093/nar/23.3.530subject
Has Abstractpub_date
1995-02-11 00:00:00pages
530-4issue
3eissn
0305-1048issn
1362-4962pii
4j0014journal_volume
23pub_type
杂志文章abstract::An attempt has been made to correlate differential scanning calorimetry melting profiles of 5S rRNAs from lupin seeds (L.s.) and wheat germ (W.g.) with their structure. It is suggested that the observed differences in thermal unfolding are due to differences in RNA nucleotide sequence and as a consequence in higher or...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.2.685
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abstract::Alternative splicing generates multiple transcript and protein isoforms from a single gene and controls transcript intracellular localization and stability by coupling to mRNA export and nonsense-mediated mRNA decay (NMD). RNA interference (RNAi) is a potent mechanism to modulate gene expression. However, its interact...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2021-01-25 00:00:00
abstract::Revealing the clonal composition of a single tumor is essential for identifying cell subpopulations with metastatic potential in primary tumors or with resistance to therapies in metastatic tumors. Sequencing technologies provide only an overview of the aggregate of numerous cells. Computational approaches to de-mix a...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.19.9097
更新日期:1988-10-11 00:00:00
abstract::Three novel DNA-binding proteins with apparent molecular masses of 7, 10 and 30 kDa have been isolated from the hyperthermophilic methanogen Methanopyrus kandleri. The proteins were identified using a blot overlay assay that was modified to emulate the high ionic strength intracellular environment of M.kandleri protei...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.3.685
更新日期:2002-02-01 00:00:00
abstract::Traditional chromatin analysis methods only test one locus at the time or use different templates for each locus, making a standardized analysis of large genomic regions or many co-regulated genes at different loci a difficult task. On the other hand, genome-wide high-resolution mapping of chromatin accessibility empl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp037
更新日期:2009-04-01 00:00:00
abstract::The strand displacement activity of DNA polymerase δ is strongly stimulated by its interaction with proliferating cell nuclear antigen (PCNA). However, inactivation of the 3'-5' exonuclease activity is sufficient to allow the polymerase to carry out strand displacement even in the absence of PCNA. We have examined in ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr235
更新日期:2011-07-01 00:00:00
abstract::Besides orthologs of other eukaryotic mismatch-repair (MMR) proteins, plants encode MSH7, a paralog of MSH6. The Arabidopsis thaliana recognition heterodimers AtMSH2*MSH6 (AtMutSalpha) and AtMSH2*MSH3 (AtMutSbeta) were previously found to bind the same subsets of mismatches as their counterparts in other eukaryotes--r...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1989-04-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::The tight-binding I12-X86 lac repressor binds to non-operator DNA in a sequence-specific fashion. Using the DNA of the E. coli I gene we have investigated these sequence-specific interactions and compared them to the operator binding of wild-type repressor. The specific, non-operator DNA interactions are sensitive to ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1980-02-11 00:00:00
abstract::Predicting molecular interactions is a major goal in rational drug design. Pharmacophore, which is the spatial arrangement of features that is essential for a molecule to interact with a specific target receptor, is an important model for achieving this goal. We present a freely available web server, named PharmaGist,...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh104
更新日期:2004-07-16 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2019-04-08 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr787
更新日期:2012-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1986-03-25 00:00:00
abstract::The UCSC Genome Browser (https://genome.ucsc.edu) provides a web interface for exploring annotated genome assemblies. The assemblies and annotation tracks are updated on an ongoing basis-12 assemblies and more than 28 tracks were added in the past year. Two recent additions are a display of CRISPR/Cas9 guide sequences...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-07-06 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1981-11-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.7.1689
更新日期:1998-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.8.1805
更新日期:1980-04-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn894
更新日期:2009-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1253
更新日期:2021-01-25 00:00:00