Abstract：
:The RES complex was previously identified in yeast as a splicing factor affecting nuclear pre-mRNA retention. This complex was shown to contain three subunits, namely Snu17, Bud13 and Pml1, but its mode of action remains ill-defined. To obtain insights into its function, we have performed a structural investigation of this factor. Production of a short N-terminal truncation of residues that are apparently disordered allowed us to determine the X-ray crystallographic structure of Pml1. This demonstrated that it consists mainly of a FHA domain, a fold which has been shown to mediate interactions with phosphothreonine-containing peptides. Using a new sensitive assay based on alternative splice-site choice, we show, however, that mutation of the putative phosphothreonine-binding pocket of Pml1 does not affect pre-mRNA splicing. We have also investigated how Pml1 integrates into the RES complex. Production of recombinant complexes, combined with serial truncation and mutagenesis of their subunits, indicated that Pml1 binds to Snu17, which itself contacts Bud13. This analysis allowed us to demarcate the binding sites involved in the formation of this assembly. We propose a model of the organization of the RES complex based on these results, and discuss the functional consequences of this architecture.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Brooks MA,Dziembowski A,Quevillon-Cheruel S,Henriot V,Faux C,van Tilbeurgh H,Séraphin Bdoi
10.1093/nar/gkn894subject
Has Abstractpub_date
2009-01-01 00:00:00pages
129-43issue
1eissn
0305-1048issn
1362-4962pii
gkn894journal_volume
37pub_type
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