Abstract:
:Traditional chromatin analysis methods only test one locus at the time or use different templates for each locus, making a standardized analysis of large genomic regions or many co-regulated genes at different loci a difficult task. On the other hand, genome-wide high-resolution mapping of chromatin accessibility employing massive parallel sequencing platforms generates an extensive data set laborious to analyse and is a cost-intensive method, only applicable to the analysis of a limited set of biological samples. To close this gap between the traditional and the high-throughput procedures we have developed a method in which a condition-specific, genome-wide chromatin fragment library is produced and then used for locus-specific DNA fragment analysis. To validate the method, we used, as a test locus, the well-studied promoter of the divergently transcribed niiA and niaD genes coding for nitrate assimilation enzymes in Aspergillus. Additionally, we have used the condition-specific libraries to study nucleosomal positioning at two different loci, the promoters of the general nitrogen regulator areA and the regulator of secondary metabolism, aflR.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Basheer A,Berger H,Reyes-Dominguez Y,Gorfer M,Strauss Jdoi
10.1093/nar/gkp037subject
Has Abstractpub_date
2009-04-01 00:00:00pages
e42issue
6eissn
0305-1048issn
1362-4962pii
gkp037journal_volume
37pub_type
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