Molecular Biology Database List.

abstract：:Fluorescence anisotropy and fluorescence correlation spectroscopy measurements of rhodamine-labeled DNA oligonucleotide duplexes have been used to determine equilibrium binding constants for DNA binding of the prokaryotic transcription activator protein NtrC. Measurements were made with wild-type NtrC from Escherichia...

journal_title：Nucleic acids research

authors： Sevenich FW,Langowski J,Weiss V,Rippe K

更新日期：1998-03-15 00:00:00

abstract：:Segments of SV40 DNA having homologous overlapping termini recombine to produce viable genomes in monkey cells. Frequencies of recombination on either side of a deletion marker are non-random; replication and palindromes do not appear to be essential. Since recombination involves host enzymes, a suitable system has be...

journal_title：Nucleic acids research

authors： Upcroft P,Carter B,Kidson C

更新日期：1980-06-25 00:00:00

abstract：:Cadmium (Cd2+) is a known carcinogen that inactivates the DNA mismatch repair (MMR) pathway. In this study, we have tested the effect of Cd2+ exposure on the enzymatic activity of the mismatch binding complex MSH2-MSH6. Our results indicate that Cd2+ is highly inhibitory to the ATP binding and hydrolysis activities of...

journal_title：Nucleic acids research

authors： Banerjee S,Flores-Rozas H

更新日期：2005-03-03 00:00:00

abstract：:Significant differences are observed between the CD spectra of eu- and heterochromatin prepared by fractionating mechanically sheared rat liver chromatin on linear sucrose density gradients. Heterochromatin has a broad positive CD at 270--280 nm, a cross-over point at 256 nmn, and a negative shoulder at 248 nm. Euchro...

journal_title：Nucleic acids research

authors： Moyer GH,Kay E,Austin GE

更新日期：1979-04-01 00:00:00

abstract：:A mathematical model and a computer simulation were used to study PCR specificity. The model describes the occurrences of non-targeted PCR products formed through random primer-template interactions. The PCR simulation scans DNA sequence databases with primers pairs. According to the model prediction, PCR with complex...

journal_title：Nucleic acids research

authors： Rubin E,Levy AA

更新日期：1996-09-15 00:00:00

abstract：:A U3 snoRNA gene isolated from a Chlamydomonas reinhardtii (CRE:) genomic library contains putative pol III-specific transcription signals similar to those of RNA polymerase III-specific small nuclear (sn)RNA genes of higher plants. The 222 nt long CRE: U3 snoRNA was immunoprecipitated by anti-gamma-mpppN antisera, bu...

journal_title：Nucleic acids research

authors： Antal M,Mougin A,Kis M,Boros E,Steger G,Jakab G,Solymosy F,Branlant C

更新日期：2000-08-01 00:00:00

abstract：:Synthetic deoxyoligonucleotides have been 5'-aminoalkylated at the end of step-wise synthesis on the polymer support. This was achieved through the activation of the 5'-hydroxyl group as its 5'-imidazolyl derivative using carbonyldiimidazole, which was subsequently displaced with hexamethylene diamine to yield the tit...

journal_title：Nucleic acids research

authors： Wachter L,Jablonski JA,Ramachandran KL

更新日期：1986-10-24 00:00:00

abstract：:Alternative splicing of mRNA precursors results in multiple protein variants from a single gene and is critical for diverse cellular processes and development. Xist encodes a long noncoding RNA which is a central player to induce X-chromosome inactivation in female mammals and has two major splicing variants: long and...

journal_title：Nucleic acids research

authors： Yue M,Ogawa Y

更新日期：2018-03-16 00:00:00

abstract：:T7 RNA polymerase promoters consist of a highly conserved 23 base-pair sequence that spans the site of the initiation of transcription (+1) and extends from -17 to +6. To determine the bases within the T7 consensus promoter that are essential for promoter function a library of mutant T7 promoters was constructed, and ...

journal_title：Nucleic acids research

authors： Ikeda RA,Ligman CM,Warshamana S

更新日期：1992-05-25 00:00:00

abstract：:RNA structural complexity and flexibility present a challenge for computational modeling efforts. Experimental information and bioinformatics data can be used as restraints to improve the accuracy of RNA tertiary structure prediction. Regarding utilization of restraints, the fundamental questions are: (i) What is the ...

journal_title：Nucleic acids research

authors： Wang J,Williams B,Chirasani VR,Krokhotin A,Das R,Dokholyan NV

更新日期：2019-06-20 00:00:00

abstract：:Peroxisomes are essential organelles of eukaryotic origin, ubiquitously distributed in cells and organisms, playing key roles in lipid and antioxidant metabolism. Loss or malfunction of peroxisomes causes more than 20 fatal inherited conditions. We have created a peroxisomal database (http://www.peroxisomeDB.org) that...

journal_title：Nucleic acids research

authors： Schlüter A,Fourcade S,Domènech-Estévez E,Gabaldón T,Huerta-Cepas J,Berthommier G,Ripp R,Wanders RJ,Poch O,Pujol A

更新日期：2007-01-01 00:00:00

abstract：:The early DNa products of reverse transcription have been analyzed from reconstructed reactions containing avian myeloblastosis virus 35S RNA . tRNAtrp complex and highly purified reverse transcriptase. We describe conditions for the synthesis of genome-length complementary DNA and two discrete species of plus strand ...

journal_title：Nucleic acids research

authors： Olsen JC,Watson KF

更新日期：1982-02-11 00:00:00

abstract：:Lnc2Meth (http://www.bio-bigdata.com/Lnc2Meth/), an interactive resource to identify regulatory relationships between human long non-coding RNAs (lncRNAs) and DNA methylation, is not only a manually curated collection and annotation of experimentally supported lncRNAs-DNA methylation associations but also a platform t...

journal_title：Nucleic acids research

authors： Zhi H,Li X,Wang P,Gao Y,Gao B,Zhou D,Zhang Y,Guo M,Yue M,Shen W,Ning S,Jin L,Li X

更新日期：2018-01-04 00:00:00

abstract：:The budding yeast Saccharomyces cerevisiae is proving to be an useful and accurate model for eukaryotic DNA replication. It contains both DNA polymerase alpha (I) and delta (III). Recently, proliferating cell nuclear antigen (PCNA), which in mammalian cells is an auxiliary subunit of DNA polymerase delta and is essent...

journal_title：Nucleic acids research

authors： Bauer GA,Burgers PM

更新日期：1990-01-25 00:00:00

abstract：:DNA triplet repeats, 5'-d(CTG)n and 5'-d(CAG)n, are present in genes which have been implicated in several neurodegenerative disorders. To investigate possible stable structures formed by these repeating sequences, we have examined d(CTG)n, d(CAG)n and d(CTG).d(CAG)n (n = 2 and 3) using NMR and UV optical spectroscopy...

journal_title：Nucleic acids research

authors： Smith GK,Jie J,Fox GE,Gao X

更新日期：1995-11-11 00:00:00

abstract：:EcoGene (http://ecogene.org) is a database and website devoted to continuously improving the structural and functional annotation of Escherichia coli K-12, one of the most well understood model organisms, represented by the MG1655(Seq) genome sequence and annotations. Major improvements to EcoGene in the past decade i...

journal_title：Nucleic acids research

authors： Zhou J,Rudd KE

更新日期：2013-01-01 00:00:00

abstract：:In prokaryotes and archaea transfer ribonucleic acid (tRNA) stability as well as cellular UV protection relies on the post-transcriptional modification of uracil at position 8 (U8) of tRNAs by the 4-thiouridine synthetase ThiI. Here, we report three crystal structures of ThiI from Thermotoga maritima in complex with a...

journal_title：Nucleic acids research

authors： Neumann P,Lakomek K,Naumann PT,Erwin WM,Lauhon CT,Ficner R

更新日期：2014-06-01 00:00:00

abstract：:The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DN...

journal_title：Nucleic acids research

authors： Casey J,Davidson N

更新日期：1977-01-01 00:00:00

abstract：:The D. melanogaster Adh gene is transcribed from two different promoters; a proximal (larval) promoter is active during late embryonic and larval stages, and a distal (adult) promoter is active primarily in third instar larvae and in adult flies (1). Genetic analyses suggest that several species of the mulleri subgrou...

journal_title：Nucleic acids research

authors： Fischer JA,Maniatis T

更新日期：1985-10-11 00:00:00

abstract：:The human RAD54B protein is a paralog of the RAD54 protein, which plays important roles in homologous recombination. RAD54B contains an N-terminal region outside the SWI2/SNF2 domain that shares less conservation with the corresponding region in RAD54. The biochemical roles of this region of RAD54B are not known, alth...

journal_title：Nucleic acids research

authors： Sarai N,Kagawa W,Fujikawa N,Saito K,Hikiba J,Tanaka K,Miyagawa K,Kurumizaka H,Yokoyama S

更新日期：2008-10-01 00:00:00

abstract：:The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophila tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initia...

journal_title：Nucleic acids research

authors： Sharp S,Dingermann T,Schaack J,Sharp JA,Burke DJ,DeRobertis EM,Söll D

更新日期：1983-12-20 00:00:00

abstract：:Alternative splicing (AS) of pre-mRNAs is an important regulatory mechanism shaping the transcriptome. In plants, only few RNA-binding proteins are known to affect AS. Here, we show that the glycine-rich RNA-binding protein AtGRP7 influences AS in Arabidopsis thaliana. Using a high-resolution RT-PCR-based AS panel, we...

journal_title：Nucleic acids research

authors： Streitner C,Köster T,Simpson CG,Shaw P,Danisman S,Brown JW,Staiger D

更新日期：2012-12-01 00:00:00

abstract：:An endo-exonuclease has been purified from cultured monkey (CV-1) cells. The enzyme which was purified to near homogeneity to be a 65 kDa monomeric protein. The single-strand DNase activity is endonucleolytic and nonprocessive, whereas the double-strand DNase activity is exonucleolytic and processive. The enzyme was a...

journal_title：Nucleic acids research

authors： Couture C,Chow TY

更新日期：1992-08-25 00:00:00

abstract：:The log kobs vs. pH profiles were determined in the intermediate acidity region for the glycosyl hydrolysis of guanosine and its 8-amino, 8-monomethylamino, 8-dimethylamino and 8-bromo derivatives. The decreased rate of the 8-amino and enhanced rate of the 8-bromo compound compared to guanosine support an A type mecha...

journal_title：Nucleic acids research

authors： Jordan F,Niv H

更新日期：1977-03-01 00:00:00

abstract：:Conformations of 8-bromo-2'-[unk]-triisopropylbenzenesulfonyladenosine ([unk]) and its 3'-[unk]-isomer ([unk]) in solution have been determined by the use of intramolecular nuclear Overhauser effects in (1)H NMR spectroscopy. Compound [unk] has been proved to have a conformation in which the adenosine and benzene ring...

journal_title：Nucleic acids research

authors： Ueyama M,Tori K,Ikehara M,Kaneko M

更新日期：1974-06-01 00:00:00

abstract：:The Mouse Genome Database (MGD) focuses on the integration of mapping, homology, polymorphism and molecular data about the laboratory mouse. Detailed descriptions of genes including their chromosomal location, gene function, disease associations, mutant phenotypes, molecular polymorphisms and links to representative s...

journal_title：Nucleic acids research

authors： Blake JA,Richardson JE,Davisson MT,Eppig JT

更新日期：1999-01-01 00:00:00

abstract：:Successful development of biological databases requires accommodation of the burgeoning amounts of data from high-throughput genomics pipelines. As the volume of curated data in Animal QTLdb (https://www.animalgenome.org/QTLdb) increases exponentially, the resulting challenges must be met with rapid infrastructure dev...

journal_title：Nucleic acids research

authors： Hu ZL,Park CA,Reecy JM

更新日期：2019-01-08 00:00:00

abstract：:We describe the further development of a widely used package of DNA/protein sequence analysis programs (1). Important revisions have been made based on user experience, and new features, multi-user capability, and a set of large scale homology programs have been added. The programs are very user friendly, economical o...

journal_title：Nucleic acids research

authors： Pustell J,Kafatos FC

更新日期：1984-01-11 00:00:00

abstract：:DISCOVER1 (DIStribution COunter VERsion 1) is a new program that can identify DNA motifs occurring with a high deviation from the expected frequency. The program generates families of patterns, each family having a common set of defined bases. Undefined bases are inserted amongst the defined bases in different ways, t...

journal_title：Nucleic acids research

authors： Valle G

更新日期：1993-11-11 00:00:00

abstract：:Yeast tRNA Val 1 alkylation with 2', 3'-O-4-(N-2-chloroethyl-N-methylamino) benzylidene d(pC-G)-A proceeds at 20 degrees - 30 degrees C in the complementary complexes which are formed by d(pC-G)-A greater than RC1 binding to 3 sequences of tRNA Val 1 : psi-C-G58 in the T loop, C-G40 at the 3'-side of the anticodon loo...

journal_title：Nucleic acids research

authors： Grineva NI,Karpova GG,Kuznetsova LM,Venkstern TV,Bayev AA

更新日期：1977-01-01 00:00:00