Abstract:
:A U3 snoRNA gene isolated from a Chlamydomonas reinhardtii (CRE:) genomic library contains putative pol III-specific transcription signals similar to those of RNA polymerase III-specific small nuclear (sn)RNA genes of higher plants. The 222 nt long CRE: U3 snoRNA was immunoprecipitated by anti-gamma-mpppN antisera, but not by anti-m(2,2,7)G antibodies, supporting the notion that it is a RNA polymerase III transcript. Tagged CRE: U3 snoRNA gene constructs were expressed in CRE: cells. Results of chemical and enzymatic structure probing of CRE: U3 snoRNA in solution and of DMS modification of CRE: U3 snoRNA under in vivo conditions revealed that the two-hairpin structure of the 5'-domain that is found in solution is no longer detected under in vivo conditions. The observed differences can be explained by the formation of several base pair interactions with the 18S and 5'-ETS parts of the pre-rRNA. A model that involves five intermolecular helices is proposed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Antal M,Mougin A,Kis M,Boros E,Steger G,Jakab G,Solymosy F,Branlant Cdoi
10.1093/nar/28.15.2959keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
2959-68issue
15eissn
0305-1048issn
1362-4962journal_volume
28pub_type
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