Abstract:
:The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophila tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initiation site is a complex mechanism that involves the T-control region, the D-control region, and sequences 5' adjacent to the D-control region. In this study either "half" of the Drosophila tRNAArg gene promoted transcription in Xenopus oocytes. This finding supports a recent model for eukaryotic tRNA gene transcription (Dingermann et al., 1983, J. Biol. Chem. 258, 10395-10402) that proposes transcription initiation is dependent on the ability of specific DNA sequences to sequester two RNA polymerase III transcription factors.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sharp S,Dingermann T,Schaack J,Sharp JA,Burke DJ,DeRobertis EM,Söll Ddoi
10.1093/nar/11.24.8677subject
Has Abstractpub_date
1983-12-20 00:00:00pages
8677-90issue
24eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract::The diverse localization of transcripts in cells suggests that there are many specific RNA-protein interactions that have yet to be identified. Progress has been limited, however, by the lack of a robust method to detect and isolate the RNA-binding proteins. Here we describe the use of an RNA aptamer, scaffolded to a ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1316
更新日期:2011-04-01 00:00:00
abstract::LOCtarget is a web server and database that predicts and annotates sub-cellular localization for structural genomics targets; LOCnet is one of the methods used in LOCtarget that can predict sub-cellular localization for all eukaryotic and prokaryotic proteins. Targets are taken from the central registration database f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh441
更新日期:2004-07-01 00:00:00
abstract::We have constructed and tested several new vectors for P element-mediated gene transfer. These vectors contain restriction sites for cloning a wide variety of DNA fragments within a small, non-autonomous P element and can be used to efficiently transduce microinjected DNA sequences into the germ line chromosomes of D....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.18.6341
更新日期:1983-09-24 00:00:00
abstract::Genetic recombination in meiosis plays an important role in generating diversity of genetic information. In yeast an Escherichia coli RecA-like gene, DMC1, is expressed in meiotic prophase and its product co-localizes with Rad51 protein on zygotene chromosomes. We have cloned the mouse and human homologs of the yeast ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.3.470
更新日期:1996-02-01 00:00:00
abstract::A novel method of determining the number of superhelical turns of a covalently-closed plasmid DNA is described. It relies on the determination of the hyperchromicity, and hence the proportion of unstacked basepairs, of a partially heat-denatured sample which co-migrates during electrophoresis with nicked circular dupl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.2.525
更新日期:1982-01-22 00:00:00
abstract::Transposition of Saccharomyces cerevisiae Ty1 retroelements has been shown to involve reverse transcription in intracytoplasmic virus-like particles (Ty-VLPs). Ty DNA present in the particles specified by Ty1-H3 element was found to consist of the full-length genomic DNA as well as incomplete cDNAs mainly of plus pola...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.15.3513
更新日期:1993-07-25 00:00:00
abstract::The RNA-Seq technology has revolutionized transcriptome characterization not only by accurately quantifying gene expression, but also by the identification of novel transcripts like chimeric fusion transcripts. The 'fusion' or 'chimeric' transcripts have improved the diagnosis and prognosis of several tumors, and have...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx315
更新日期:2017-07-27 00:00:00
abstract::Conditional gene targeting depends on tissue and time specificity of recombination events. Endogenous promoters are often used to drive various transgenic constructs. To avoid the problems associated with reconstituting a specific expression pattern in transgenic animals by this method, we tested the internal ribosome...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.6.1552
更新日期:1999-03-15 00:00:00
abstract::Naturally occurring RNAs are known to exhibit a high degree of modularity, whereby specific structural modules (or motifs) can be mixed and matched to create new molecular architectures. The modular nature of RNA also affords researchers the ability to characterize individual structural elements in controlled syntheti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz304
更新日期:2019-07-09 00:00:00
abstract::The Pk-rec gene, encoding a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcussp. KOD1, was expressed in Escherichia coli. The recombinant Pk-REC was purified to homogeneity and was shown to be in a dimeric form. A striking property of the purified recombinant Pk-REC was the unusual DNase activity on ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.4.719
更新日期:1997-02-15 00:00:00
abstract::Primer Design Assistant (PDA) is a web interface primer design service combined with thermodynamic theory to evaluate the fitness of primers. It runs in a Linux-Apache-MySQL-PHP structure on a PC equipped with dual CPU (Intel Pentium III 1.4 GHz) and 512 Mb of RAM. A succinct user interface of PDA is accomplished by b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg560
更新日期:2003-07-01 00:00:00
abstract::The complete DNA sequence of pO157, the large virulence plasmid of EHEC strain O157:H7 EDL 933, is presented. The 92 kb F-like plasmid is composed of segments of putative virulence genes in a framework of replication and maintenance regions, with seven insertion sequence elements, located mostly at the boundaries of t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.18.4196
更新日期:1998-09-15 00:00:00
abstract::In addition to maintaining the GenBank(R) nucleic acid sequence database, the National Center for Biotechnology Information (NCBI) provides analysis and retrieval resources for the data in GenBank and other biological data available through NCBI's web site. NCBI resources include Entrez, the Entrez Programming Utiliti...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1000
更新日期:2008-01-01 00:00:00
abstract::Cationic aromatic tricyclic compounds including triphenylmethane dyes, phenazines, phenoxazines, acridines, phenothiazines, phenanthridinium compounds, anthracenes and xanthene dyes, which amplify cell killing in phleomycin-treated Escherichia coli B cells also modified phleomycin-induced breakdown of DNA to acid-solu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.23.9083
更新日期:1984-12-11 00:00:00
abstract::The SIGnaling Network Open Resource 2.0 (SIGNOR 2.0) is a public repository that stores signaling information as binary causal relationships between biological entities. The captured information is represented graphically as a signed directed graph. Each signaling relationship is associated to an effect (up/down-regul...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz949
更新日期:2020-01-08 00:00:00
abstract::Escherichia coli DNA polymerase III holoenzyme is composed of 10 different subunits linked by noncovalent interactions. The polymerase activity resides in the alpha-subunit. The epsilon-subunit, which contains the proofreading exonuclease site within its N-terminal 185 residues, binds to alpha via a segment of 57 addi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn489
更新日期:2008-09-01 00:00:00
abstract::MicA is a trans-encoded small non-coding RNA, which downregulates porin-expression in stationary-phase. In this work, we focus on the role of endoribonucleases III and E on Salmonella typhimurium sRNA MicA regulation. RNase III is shown to regulate MicA in a target-coupled way, while RNase E is responsible for the con...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1239
更新日期:2011-04-01 00:00:00
abstract::Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.18.8835
更新日期:1988-09-26 00:00:00
abstract::Guanine-rich sequences are highly abundant in the human genome, especially in regulatory regions. Because guanine-rich sequences have the unique ability to form G-quadruplexes, these structures may play a role in the regulation of gene transcription. In previous studies, we demonstrated that formation of G-quadruplexe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr259
更新日期:2011-09-01 00:00:00
abstract::Non-coding genetic variants/mutations can play functional roles in the cell by disrupting regulatory interactions between transcription factors (TFs) and their genomic target sites. For most human TFs, a myriad of DNA-binding models are available and could be used to predict the effects of DNA mutations on TF binding....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz363
更新日期:2019-07-02 00:00:00
abstract::Minimized trans-acting HDV ribozyme systems consisting of three (Rz-3) and two (Rz-2) RNA strands were prepared and their folding conformations were analyzed by NMR spectroscopy. The guanosine residues in one of the enzyme components of Rz-3 were labeled with 13C and 15N. Imino proton signals were assigned by analysis...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.3.766
更新日期:2002-02-01 00:00:00
abstract::We have determined the nucleotide sequence of a 7.5 kb full-size gypsy element from Drosophila subobscura strain H-271. Comparative analyses were carried out on the sequence and molecular structure of gypsy elements of D.subobscura (gypsyDs), D.melanogaster (gypsyDm) and D.virilis (gypsyDv). The three elements show a ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.5.914
更新日期:1996-03-01 00:00:00
abstract::We have isolated a rat cDNA, named FE65, hybridizing to an mRNA of about 2,300 nucleotides present in rat brain, undetectable in rat liver and very poorly represented in other tissues. An mRNA of the same size is present in human neuroblastoma cells and is absent from other human cell lines. The FE65 cDNA contains an ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.19.5269
更新日期:1991-10-11 00:00:00
abstract::High-resolution structures of proteins remain the most valuable source for understanding their function in the cell and provide leads for drug design. Since the availability of sufficient protein structures to tackle complex problems such as modeling backbone moves or docking remains a problem, alternative approaches ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq972
更新日期:2011-01-01 00:00:00
abstract::During the last years, the demand for custom-made cDNA chips/arrays as well as whole genome chips is increasing rapidly. The efficient selection of gene-specific primers/oligomers is of the utmost importance for the successful production of such chips. We developed GenomePRIDE, a highly flexible and scalable software ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg752
更新日期:2003-10-01 00:00:00
abstract::A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with its cognate and non cognate yeast tRNAs. It was shown that, when limited amounts of tRNA (either cognate or non cognate) a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.7.2421
更新日期:1977-07-01 00:00:00
abstract::Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.10.3255
更新日期:1983-05-25 00:00:00
abstract::The 5'-flanking region of the bovine prolactin gene was cloned and sequenced. The expression of chimeric gene constructs containing 5'-flanking DNA fragments from the prolactin gene joined to a reporter gene encoding human growth hormone (hGH) was examined using transiently transfected rat pituitary cells. Prolactin n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:
更新日期:1990-08-25 00:00:00
abstract::We have sequenced the 0.7-kb-long fragment of Drosophila DNA which ensures the autonomous replication of plasmids in yeast. Deletion mapping has shown the ARS element to consist of at least two domains: the core having the consensus 11-bp sequence TAAATATAAAT and the enhancer which is no more than 90 bp long and is lo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.3.1169
更新日期:1988-02-11 00:00:00
abstract::The Plant Ontology Consortium (POC, http://www.plantontology.org) is a collaborative effort among model plant genome databases and plant researchers that aims to create, maintain and facilitate the use of a controlled vocabulary (ontology) for plants. The ontology allows users to ascribe attributes of plant structure ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm908
更新日期:2008-01-01 00:00:00