Abstract:
:Transposition of Saccharomyces cerevisiae Ty1 retroelements has been shown to involve reverse transcription in intracytoplasmic virus-like particles (Ty-VLPs). Ty DNA present in the particles specified by Ty1-H3 element was found to consist of the full-length genomic DNA as well as incomplete cDNAs mainly of plus polarity. Our results indicate that identical sequences (TGGGTGGTA) are used as primers for the synthesis of plus strand cDNA, generating cDNAs of 0.345 kb (analogous to the retroviral strong-stop plus cDNA) and of 2.1 kb. Electron microscopic analyses of Ty1-VLP DNA revealed two distinct classes, one full-length and the other corresponding to 0.34 kbp molecules, the size of a LTR sequence. The full-length molecules are either completely double-stranded or only partially double- stranded at one end or at both ends. These double-stranded regions are of a length corresponding to those of incomplete plus strands detected by biochemical techniques. Double-stranded circular molecules mainly of a length corresponding to that of two-LTR circles were also detected on electron micrographs. These analyses allowed us to propose a scheme for reverse transcription in Ty particles.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Pochart P,Agoutin B,Rousset S,Chanet R,Doroszkiewicz V,Heyman Tdoi
10.1093/nar/21.15.3513subject
Has Abstractpub_date
1993-07-25 00:00:00pages
3513-20issue
15eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract::Immunoglobulin genes contain a conserved eight base sequence element 5' to the site of transcription initiation. This octamer can serve as a site for the binding of nuclear proteins which are presumably involved in the cell type specific expression of this family of genes. In studying the binding of nuclear proteins t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.12.5503
更新日期:1988-06-24 00:00:00
abstract::The CABS-fold web server provides tools for protein structure prediction from sequence only (de novo modeling) and also using alternative templates (consensus modeling). The web server is based on the CABS modeling procedures ranked in previous Critical Assessment of techniques for protein Structure Prediction competi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt462
更新日期:2013-07-01 00:00:00
abstract::We previously showed that mRNAs synthesized from three genes that naturally lack introns contain a portion of their coding sequence, known as a cytoplasmic accumulation region (CAR), which is essential for stable accumulation of the intronless mRNAs in the cytoplasm. The CAR in each mRNA is unexpectedly large, ranging...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1314
更新日期:2013-02-01 00:00:00
abstract::We report the consensus sequence of members of a new dispersed middle-repetitive DNA family, MT, which is present in mouse and rat genomes. This family is shown to be as abundant as the described rodent B1 and B2 families. Hybridization experiments with radioactive single-stranded cDNAs from different tissues indicate...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.16.6403
更新日期:1986-08-26 00:00:00
abstract::DNA double-strand breaks are repaired by end-joining or homologous recombination. A key-committing step of recombination is DNA end resection. In resection, phosphorylated CtIP first promotes the endonuclease of MRE11-RAD50-NBS1 (MRN). Subsequently, CtIP also stimulates the WRN/BLM-DNA2 pathway, coordinating thus both...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa273
更新日期:2020-06-04 00:00:00
abstract::TATA-binding protein (TBP) is central to the regulation of eukaryotic transcription initiation. Recruitment of TBP to target genes can be positively regulated by one of two basal transcription factor complexes: SAGA or TFIID. Negative regulation of TBP promoter association can be performed by Mot1p or the NC2 complex....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr784
更新日期:2012-02-01 00:00:00
abstract::Hepatitis B virus (HBV) infection remains a major health problem worldwide. The role played by microRNAs (miRNAs) in HBV replication and pathogenesis is being increasingly recognized. In this study, we found that miR-15b, an important miRNA during HBV infection and hepatocellular carcinoma development, directly binds ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku260
更新日期:2014-06-01 00:00:00
abstract::The thermodynamics of 13 hybridization reactions between 10 base DNA sequences of design 5'-ATGCXYATGC-3' with X, Y = A, C, G, T and their complementary PNA and DNA sequences were determined from isothermal titration calorimetry (ITC) measurements at ambient temperature. For the PNA/DNA hybridization reactions, the bi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.24.4792
更新日期:1999-12-15 00:00:00
abstract::A physical mapping strategy has been developed to verify and accelerate the assembly and gap closure phase of a microbial genome shotgun-sequencing project. The protocol was worked out during the ongoing Pseudomonas putida KT2440 genome project. A macro-restriction map was constructed by linking probe hybridisation of...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.22.e110
更新日期:2001-11-15 00:00:00
abstract::We describe improve enzymatic methods for sequencing method for sequencing DNA. They are based on partial digestion of duplex DNA with exonuclease III to produce DNA molecules with 3' ends shortened to varying lengths, followed by repair synthesis to extend and label the 3' ends. After asymmetrical cleavage of the DNA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.6.2065
更新日期:1982-03-25 00:00:00
abstract::This paper describes a simple program that uses a digitizing device to enter DNA sequences directly from autoradiographs into a computer. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part2.499
更新日期:1984-01-11 00:00:00
abstract::A circular trans-acting ribozyme designed to adopt the motif of the hepatitis delta virus (HDV) trans-acting ribozyme was produced. The circular form was generated in vitro by splicing a modified group I intron precursor RNA in which the relative order of the 5' and 3' splice sites, flanking the single HDV-like ribozy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.18.4253
更新日期:1993-09-11 00:00:00
abstract::The last few years have witnessed significant changes in Pfam (https://pfam.xfam.org). The number of families has grown substantially to a total of 17,929 in release 32.0. New additions have been coupled with efforts to improve existing families, including refinement of domain boundaries, their classification into Pfa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky995
更新日期:2019-01-08 00:00:00
abstract::The attachment of replicating DNA to a rapidly sedimenting nuclear structure was investigated by digestion with various nucleases. When DNA was gradually removed by DNase I, pulse label incorporated during either 1 min or during 1 hour in the presence of arabinosylcytosine, remained preferentially attached to the nucl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.1.219
更新日期:1979-01-01 00:00:00
abstract::From the general views of the eukaryotic transcription systems, L1 (or L1-like) retrotransposons that encode some proteins are unusual. L1, unlike other protein-coding elements, is transcribed through an internal promoter. And the L1 internal promoter, unlike other internal promoters, is thought to be RNA polymerase I...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.18.3704
更新日期:1995-09-25 00:00:00
abstract::In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks164
更新日期:2012-06-01 00:00:00
abstract::In the last decade polycistronic vectors have become essential tools for both basic science and gene therapy applications. In order to co-express heterologous polypeptides, different systems have been developed from Internal Ribosome Entry Site (IRES) based vectors to the use of the 2A peptide. Unfortunately, these me...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp716
更新日期:2009-11-01 00:00:00
abstract::The frequencies of occurrence of four bases in the first, second and third codon positions and in the total coding sequences have been calculated by the codon usage table published in 1990 by Ikemura et al. The distribution of frequencies are further analysed in detail by a graphic technique presented recently by us. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.22.6313
更新日期:1991-11-25 00:00:00
abstract::Hexokinase 2 is an essential factor for signalling repression through the Saccharomyces cerevisiae high-glucose sensing pathway. The main regulatory mechanism that controls the HXK2 gene expression in yeast is mediated by the Rgt1 and Med8 transcription factors, which repress HXK2 expression in low-glucose containing ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl028
更新日期:2006-03-09 00:00:00
abstract::The enzyme endonuclease V nicks uracil-containing DNA at the second or third phosphodiester bond 3' to uracil sites. I applied the enzyme to random fragmentation of DNA to revise the complex DNA shuffling protocol. The merit of using endonuclease V is that cleavage occurs at random sites and the length of the fragment...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnf139
更新日期:2002-12-15 00:00:00
abstract::The deoxyribose hexanucleoside pentaphosphate (m5dC-dG)3 has been studied by 500 MHz 1H NMR in D2O (0.1 M NaCl) and in D2O/deuterated methanol mixtures. Two conformations, in slow equilibrium on the NMR time scale, were detected in methanolic solution. Two-dimensional nuclear Overhauser effect (NOE) experiments were u...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.2.1243
更新日期:1984-01-25 00:00:00
abstract::Apolipoprotein B (apoB) mRNA editing is a nuclear event that minimally requires the RNA substrate, APOBEC-1 and APOBEC-1 Complementation Factor (ACF). The co-localization of these macro-molecules within the nucleus and the modulation of hepatic apoB mRNA editing activity have been described following a variety of meta...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl417
更新日期:2006-07-04 00:00:00
abstract::Stability of aromatic phosphoramidates was studied using 2',3'-O-dibenzoyluridine 5'-phosphoramidates and N,2',3'-O-tribenzoylcytidine 5'-phosphate. The effect of dicyclohexylcarbodiimide in this mixture was investigated. Decomposition of the anilidate was slower in the presence of DCC. Substituted anilidates of uridi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/1.2.223
更新日期:1974-02-01 00:00:00
abstract::The Retinol-Binding Protein (RBP) is expressed primarily in the liver. The regulatory elements involved in its tissue-specific expression have been identified and mapped to the 5' flanking region of the RBP gene. In this paper heterokaryons and somatic cell-hybrids have been produced and analysed in order to demonstra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.24.7235
更新日期:1990-12-25 00:00:00
abstract::Escherichia coli MutY is an adenine and a weak guanine DNA glycosylase involved in reducing mutagenic effects of 7,8-dihydro-8-oxo-guanine (8-oxoG). The C-terminal domain of MutY is required for 8-oxoG recognition and is critical for mutation avoidance of oxidative damage. To determine which residues of this domain ar...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg434
更新日期:2003-06-15 00:00:00
abstract::DXZ4 is an X-linked macrosatellite composed of 12-100 tandemly arranged 3-kb repeat units. In females, it adopts opposite chromatin arrangements at the two alleles in response to X-chromosome inactivation. In males and on the active X chromosome, it is packaged into heterochromatin, but on the inactive X chromosome (X...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr964
更新日期:2012-02-01 00:00:00
abstract::The structure of a ribonuclease III processing signal from bacteriophage T7 was examined by NMR spectroscopy, optical melting, and chemical and enzymatic modification. A 41 nucleotide variant of the T7 R1.1 processing signal has two Watson-Crick base-paired helices separated by an internal loop, consistent with its pr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.4.604
更新日期:1994-02-25 00:00:00
abstract::The class II genes of the major histocompatibility complex (MHC) encode a family of cell surface glycoproteins that present processed antigen to the T cell receptor. Class II genes are regulated coordinately, responding to both immunologic and developmental signals. Conserved sequence elements 5' to class II genes hav...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.6.1243
更新日期:1991-03-25 00:00:00
abstract::Site-directed, Ligase-Independent Mutagenesis (SLIM) is a novel PCR-mediated mutagenesis approach that can accommodate all three sequence modification types (insertion, deletion and substitution). The method utilizes an inverse PCR amplification of the template by two tailed long primers and two short primers in a sin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh172
更新日期:2004-12-07 00:00:00
abstract:T:Activator protein 1 (AP-1) is a transcription factor that recognizes two versions of a 7-base pair response element, either 5΄- GAG CA-3΄ or 5΄- GAG CA-3΄ (where M = 5-methylcytosine). These two elements share the feature that 5-methylcytosine and thymine both have a methyl group in the same position, 5-carbon of the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx057
更新日期:2017-03-17 00:00:00