Incorporation of 2'-amido-nucleosides in oligodeoxynucleotides and oligoribonucleotides as a model for 2'-linked conjugates.

abstract：:The pol gene of all retroviruses is expressed as a gag-pol fusion protein which is proteolytically processed to produce all viral enzymes. In the human immunodeficiency virus (HIV), the gag and pol genes overlap by 241 nucleotides with pol in the -1 phase with respect to gag. The gag-pol fusion is produced via a -1 ri...

journal_title：Nucleic acids research

authors： Vickers TA,Ecker DJ

更新日期：1992-08-11 00:00:00

abstract：:GenBank (R) is a comprehensive database that contains publicly available nucleotide sequences for more than 260 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the web-based BankIt or st...

journal_title：Nucleic acids research

authors： Benson DA,Karsch-Mizrachi I,Lipman DJ,Ostell J,Wheeler DL

更新日期：2008-01-01 00:00:00

abstract：:The DINAMelt web server simulates the melting of one or two single-stranded nucleic acids in solution. The goal is to predict not just a melting temperature for a hybridized pair of nucleic acids, but entire equilibrium melting profiles as a function of temperature. The two molecules are not required to be complementa...

journal_title：Nucleic acids research

authors： Markham NR,Zuker M

更新日期：2005-07-01 00:00:00

abstract：:A novel method combining elements of suppression subtractive hybridization with high throughput differential screening permits the efficient and rapid cloning of rarely transcribed differentially expressed genes. The experimental strategy virtually excludes the possibility of isolating false positive clones. The poten...

journal_title：Nucleic acids research

authors： von Stein OD,Thies WG,Hofmann M

更新日期：1997-07-01 00:00:00

abstract：:Next-generation sequencing is excellently suited to evaluate the abundance of mRNAs to study gene expression. Here we compare two alternative technologies, cap analysis of gene expression (CAGE) and serial analysis of gene expression (SAGE), for the same RNA samples. Along with quantifying gene expression levels, CAGE...

journal_title：Nucleic acids research

authors： Hestand MS,Klingenhoff A,Scherf M,Ariyurek Y,Ramos Y,van Workum W,Suzuki M,Werner T,van Ommen GJ,den Dunnen JT,Harbers M,'t Hoen PA

更新日期：2010-09-01 00:00:00

abstract：:The noncoding RNA database (ncRNAdb) was created as a source of information on RNA molecules, which do not possess protein-coding capacity. It is now widely accepted that, in addition to constitutively expressed, housekeeping or infrastructural RNAs, there is a wide variety of RNAs participating in mechanisms involved...

journal_title：Nucleic acids research

authors： Szymanski M,Erdmann VA,Barciszewski J

更新日期：2007-01-01 00:00:00

abstract：:DynOmics (dynomics.pitt.edu) is a portal developed to leverage rapidly growing structural proteomics data by efficiently and accurately evaluating the dynamics of structurally resolved systems, from individual molecules to large complexes and assemblies, in the context of their physiological environment. At the core o...

journal_title：Nucleic acids research

authors： Li H,Chang YY,Lee JY,Bahar I,Yang LW

更新日期：2017-07-03 00:00:00

abstract：:The Zebrafish Information Network (ZFIN) (https://zfin.org/) is the database for the model organism, zebrafish (Danio rerio). ZFIN expertly curates, organizes, and provides a wide array of zebrafish genetic and genomic data, including genes, alleles, transgenic lines, gene expression, gene function, mutant phenotypes,...

journal_title：Nucleic acids research

authors： Howe DG,Ramachandran S,Bradford YM,Fashena D,Toro S,Eagle A,Frazer K,Kalita P,Mani P,Martin R,Moxon ST,Paddock H,Pich C,Ruzicka L,Schaper K,Shao X,Singer A,Van Slyke CE,Westerfield M

更新日期：2021-01-08 00:00:00

abstract：:Global run-on sequencing (GRO-seq) is a recent addition to the series of high-throughput sequencing methods that enables new insights into transcriptional dynamics within a cell. However, GRO-sequencing presents new algorithmic challenges, as existing analysis platforms for ChIP-seq and RNA-seq do not address the uniq...

journal_title：Nucleic acids research

authors： Allison KA,Kaikkonen MU,Gaasterland T,Glass CK

更新日期：2014-02-01 00:00:00

abstract：:CSP41 is a ubiquitous chloroplast endoribonuclease belonging to the short chain dehydrogenase/reductase (SDR) superfamily. To help elucidate the role of CSP41 in chloroplast gene regulation, the mechanisms that determine its substrate recognition and catalytic activity were investigated. A divalent metal is required f...

journal_title：Nucleic acids research

authors： Bollenbach TJ,Stern DB

更新日期：2003-08-01 00:00:00

abstract：:The topoisomerase I (TOP1) inhibitor irinotecan triggers cell death by trapping TOP1 on DNA, generating cytotoxic protein-linked DNA breaks (PDBs). Despite its wide application in a variety of solid tumors, the mechanisms of cancer cell resistance to irinotecan remains poorly understood. Here, we generated colorectal ...

journal_title：Nucleic acids research

authors： Meisenberg C,Ashour ME,El-Shafie L,Liao C,Hodgson A,Pilborough A,Khurram SA,Downs JA,Ward SE,El-Khamisy SF

更新日期：2017-02-17 00:00:00

abstract：:Specific DNA restriction endonuclease fragments can be identified after electrophoresis in agarose gels by hybridization in the gel (in situ) to radioactive homologous RNA. RNA-DNA hybrids are detected by autoradiography of the gel. Comparison of band patterns of the autoradiogram and the ethidium bromide stained gel ...

journal_title：Nucleic acids research

authors： Shinnick TM,Lund E,Smithies O,Blattner FR

更新日期：1975-10-01 00:00:00

abstract：:Although the involvement of Ser/Arg-rich (SR) proteins in RNA metabolism is well documented, their role in vertebrate development remains elusive. We, therefore, elected to take advantage of the zebrafish model organism to study the SR genes' functions using the splicing morpholino (sMO) microinjection and the program...

journal_title：Nucleic acids research

authors： Joris M,Schloesser M,Baurain D,Hanikenne M,Muller M,Motte P

更新日期：2017-09-19 00:00:00

abstract：:The possible role of LG-1, a Tetrahymena specific HMG protein found in the macronuclear chromatin (Hamana, K. and Iwai, K. (1979) J. Biochem. 86, 789-794), was examined in relation to the chromatin structure. The chromatin isolated from cells synchronized at different stages of the cell cycle contained about one molec...

journal_title：Nucleic acids research

authors： Hamana K,Zama M

更新日期：1980-11-25 00:00:00

abstract：:Oligo(dG).oligo(dC)- or short poly(dG).poly(dC)-containing fragments were enriched and cloned by means of Mg2+-dependent triplex affinity capture and subsequent cloning procedures. A library constructed after three cycles of enrichment showed that approximately 80% of the clones in the supercoiled form formed a comple...

journal_title：Nucleic acids research

authors： Nishikawa N,Kanda N,Oishi M,Kiyama R

更新日期：1997-05-01 00:00:00

abstract：:Bacterial retrons consist of a reverse transcriptase (RT) and a contiguous non-coding RNA (ncRNA) gene. One third of annotated retrons carry additional open reading frames (ORFs), the contribution and significance of which in retron biology remains to be determined. In this study we developed a computational pipeline ...

journal_title：Nucleic acids research

authors： Mestre MR,González-Delgado A,Gutiérrez-Rus LI,Martínez-Abarca F,Toro N

更新日期：2020-12-16 00:00:00

abstract：:Multifunctional proteins challenge the conventional 'one protein-one function' paradigm. Here we note apparent multifunctional proteins with nucleic acid partners, tabulating eight examples. We then focus on eight additional cases of transcription factors that bind double-stranded DNA with sequence specificity, but th...

journal_title：Nucleic acids research

authors： Cassiday LA,Maher LJ 3rd

更新日期：2002-10-01 00:00:00

abstract：:The availability of protein fluorophores with appropriate spectral properties has made it possible to employ fluorescence resonance energy transfer (FRET) to assess interactions between three proteins microscopically. Flow cytometry offers excellent sensitivity, effective signal separation and the capacity to assess a...

journal_title：Nucleic acids research

authors： He L,Wu X,Simone J,Hewgill D,Lipsky PE

更新日期：2005-04-01 00:00:00

abstract：:We have microinjected genomic DNA clones containing the Moloney murine leukemia virus (M-MuLV) proviral genome and flanking mouse sequences from Mov-3, Mov-7 and Mov-10 mice into Xenopus laevis oocytes and analyzed the virus-specific transcription and translation products. These mouse strains carry a proviral genome c...

journal_title：Nucleic acids research

authors： Breindl M,Kalthoff H,Jaenisch R

更新日期：1983-06-25 00:00:00

abstract：:In metazoan cells, only a limited number of mini chromosome maintenance (MCM) complexes are fired during S phase, while the majority remain dormant. Several methods have been used to map replication origins, but such methods cannot identify dormant origins. Herein, we determined MCM7-binding sites in human cells using...

journal_title：Nucleic acids research

authors： Sugimoto N,Maehara K,Yoshida K,Ohkawa Y,Fujita M

更新日期：2018-07-27 00:00:00

abstract：:We present web servers for analysis of non-coding RNA sequences on the basis of their secondary structures. Software tools for structural multiple sequence alignments, structural pairwise sequence alignments and structural motif findings are available from the integrated web server and the individual stand-alone web s...

journal_title：Nucleic acids research

authors： Asai K,Kiryu H,Hamada M,Tabei Y,Sato K,Matsui H,Sakakibara Y,Terai G,Mituyama T

更新日期：2008-07-01 00:00:00

abstract：:The continuing threat of infectious disease and future pandemics, coupled to the continuous increase of drug-resistant pathogens, makes the discovery of new and better vaccines imperative. For effective vaccine development, antigen discovery and validation is a prerequisite. The compilation of information concerning p...

journal_title：Nucleic acids research

authors： Ansari HR,Flower DR,Raghava GP

更新日期：2010-01-01 00:00:00

abstract：:Chicken-erythrocyte inner histone tetramer has been complexed with several natural and synthetic DNA duplexes by salt-gradient dialysis at various protein/DNA ratios. The resulting complexes, in low-ionic-strength buffer, have been examined by electron microscopy, circular dichroism, and thermal denaturation. Electron...

journal_title：Nucleic acids research

authors： Bryan PN,Wright EB,Hsie MH,Olins AL,Olins DE

更新日期：1978-10-01 00:00:00

abstract：:Basic region-leucine zipper (B-ZIP) proteins are a class of dimeric sequence-specific DNA-binding proteins unique to eukaryotes. We have identified 67 B-ZIP proteins in the Arabidopsis thaliana genome. No A.thaliana B-ZIP domains are homologous with any Homo sapiens B-ZIP domains. Here, we predict the dimerization spe...

journal_title：Nucleic acids research

authors： Deppmann CD,Acharya A,Rishi V,Wobbes B,Smeekens S,Taparowsky EJ,Vinson C

更新日期：2004-06-29 00:00:00

abstract：:Leishmania species are protozoan parasites whose remarkably plastic genome limits the establishment of effective genetic manipulation and leishmaniasis treatment. The strategies used by Leishmania to maintain its genome while allowing variability are not fully understood. Here, we used DiCre-mediated conditional gene ...

journal_title：Nucleic acids research

authors： Damasceno JD,Obonaga R,Silva GLA,Reis-Cunha JL,Duncan SM,Bartholomeu DC,Mottram JC,McCulloch R,Tosi LRO

更新日期：2018-12-14 00:00:00

abstract：:Given that greater than 90% of the human genome is expressed, it is logical to assume that post-transcriptional regulatory mechanisms must be the primary means of controlling the flow of information from mRNA to protein. This report describes a robust approach that includes in silico, in vitro and in cellulo experimen...

journal_title：Nucleic acids research

authors： Beaudoin JD,Perreault JP

更新日期：2010-11-01 00:00:00

abstract：:A method for quantifying the proportion of supercoiled circular (SC) forms in DNA solutions is described. The method (SCFluo) takes advantage of the reversible denaturation property of SC forms and the high specificity of the PicoGreen fluorochrome for double-stranded (ds)DNA. Fluorescence values of forms capable of r...

journal_title：Nucleic acids research

authors： Levy MS,Lotfian P,O'Kennedy R,Lo-Yim MY,Shamlou PA

更新日期：2000-06-15 00:00:00

abstract：:Alternative splicing of mRNA precursors results in multiple protein variants from a single gene and is critical for diverse cellular processes and development. Xist encodes a long noncoding RNA which is a central player to induce X-chromosome inactivation in female mammals and has two major splicing variants: long and...

journal_title：Nucleic acids research

authors： Yue M,Ogawa Y

更新日期：2018-03-16 00:00:00

abstract：:Human telomerase is a ribonucleoprotein which uses its internal RNA moiety as a template for telomeric DNA synthesis. This enzyme is up-regulated in most malignant tumors and is therefore considered as a possible cancer target. Here we examined the effects of differently modified oligomers on telomeraseactivity from H...

journal_title：Nucleic acids research

authors： Matthes E,Lehmann C

更新日期：1999-02-15 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that mediate gene expression at the post-transcriptional and translational levels by an imperfect binding to target mRNA 3'UTR regions. While the ab-initio computational prediction of miRNA-mRNA interactions still poses significant challenges, it is possible to overcome som...

journal_title：Nucleic acids research

authors： Sales G,Coppe A,Bicciato S,Bortoluzzi S,Romualdi C

更新日期：2010-04-01 00:00:00