Three new members of the RNP protein family in Xenopus.

abstract：:The Chemical Effects in Biological Systems database (CEBS) is a comprehensive and unique toxicology resource that compiles individual and summary animal data from the National Toxicology Program (NTP) testing program and other depositors into a single electronic repository. CEBS has undergone significant updates in re...

journal_title：Nucleic acids research

authors： Lea IA,Gong H,Paleja A,Rashid A,Fostel J

更新日期：2017-01-04 00:00:00

abstract：:The ribosomal proteins L4 and L22 form part of the peptide exit tunnel in the large ribosomal subunit. In Escherichia coli, alterations in either of these proteins can confer resistance to the macrolide antibiotic, erythromycin. The structures of the 30S as well as the 50S subunits from each antibiotic resistant mutan...

journal_title：Nucleic acids research

authors： O'Connor M,Gregory ST,Dahlberg AE

更新日期：2004-10-27 00:00:00

abstract：:The Blocks Database contains multiple alignments of conserved regions in protein families which can be searched by e-mail (blocks@blocks.fhcrc.org) and World Wide Web (http://blocks.fhcrc.org/ ) servers to classify protein and nucleotide sequences. Recent enhancements to the servers include: (i) improved calculation o...

journal_title：Nucleic acids research

authors： Henikoff JG,Pietrokovski S,Henikoff S

更新日期：1997-01-01 00:00:00

abstract：:A simple and effective modified ethanol precipitation-based protocol is described for the preparation of DNA from ancient human bones. This method is fast and requires neither hazardous chemicals nor special devices. After the powdering and incubating of the bone samples Dextran Blue was added as a carrier for removin...

journal_title：Nucleic acids research

authors： Kalmár T,Bachrati CZ,Marcsik A,Raskó I

更新日期：2000-06-15 00:00:00

abstract：:Message-specific translational regulation mechanisms shape the biogenesis of multimeric oxidative phosphorylation (OXPHOS) enzyme in mitochondria from the yeast Saccharomyces cerevisiae. These mechanisms, driven mainly by the action of mRNA-specific translational activators, help to coordinate synthesis of OXPHOS cata...

journal_title：Nucleic acids research

authors： Mays JN,Camacho-Villasana Y,Garcia-Villegas R,Perez-Martinez X,Barrientos A,Fontanesi F

更新日期：2019-06-20 00:00:00

abstract：:Mononegaviruses, such as Ebola virus, encode an L (large) protein that bears all the catalytic activities for replication/transcription and RNA capping. The C-terminal conserved region VI (CRVI) of L protein contains a K-D-K-E catalytic tetrad typical for 2'O methyltransferases (MTase). In mononegaviruses, cap-MTase a...

journal_title：Nucleic acids research

authors： Martin B,Coutard B,Guez T,Paesen GC,Canard B,Debart F,Vasseur JJ,Grimes JM,Decroly E

更新日期：2018-09-06 00:00:00

abstract：:The U1 snRNP-specific protein C contains an N-terminal zinc finger-like CH motif which is required for the binding of the U1C protein to the U1 snRNP particle. Recently a similar motif was reported to be essential for in vivo homodimerization of the yeast splicing factor PRP9. In the present study we demonstrate that ...

journal_title：Nucleic acids research

authors： Gunnewiek JM,van Aarssen Y,Wassenaar R,Legrain P,van Venrooij WJ,Nelissen RL

更新日期：1995-12-11 00:00:00

abstract：:5-methylcytosine (m5C) modifications of RNA are ubiquitous in nature and play important roles in many biological processes such as protein translational regulation, RNA processing and stress response. Aberrant expressions of RNA:m5C methyltransferases are closely associated with various human diseases including cancer...

journal_title：Nucleic acids research

authors： Liu RJ,Long T,Li J,Li H,Wang ED

更新日期：2017-06-20 00:00:00

abstract：:The accurate calculation of protein/nucleic acid-ligand interactions or condensed phase properties by force field-based methods require a precise description of the energetics of intermolecular interactions. Despite the progress made in force fields, small molecule parameterization remains an open problem due to the m...

journal_title：Nucleic acids research

authors： Dodda LS,Cabeza de Vaca I,Tirado-Rives J,Jorgensen WL

更新日期：2017-07-03 00:00:00

abstract：:A number of cysteine-rich proteins have recently been isolated by homology screening, differential library screens, and association with other proteins. In this report, we describe the isolation of the rat cysteine-rich protein from a rat brain library during a search for clones with homology to the delta-opioid recep...

journal_title：Nucleic acids research

authors： McLaughlin CR,Tao Q,Abood ME

更新日期：1994-12-11 00:00:00

abstract：:Here we compare several methods for predicting oligonucleotide frequencies in 691 kb of Drosophila melanogaster DNA. As in previous work on Escherichia coli and Saccharomyces cerevisiae, a relatively simple equation based on tetranucleotide frequencies can be used in predicting frequencies of higher order oligonucleot...

journal_title：Nucleic acids research

authors： Cuticchia AJ,Ivarie R,Arnold J

更新日期：1992-07-25 00:00:00

abstract：:The UCSC Genome Browser (https://genome.ucsc.edu) provides a web interface for exploring annotated genome assemblies. The assemblies and annotation tracks are updated on an ongoing basis-12 assemblies and more than 28 tracks were added in the past year. Two recent additions are a display of CRISPR/Cas9 guide sequences...

journal_title：Nucleic acids research

authors： Casper J,Zweig AS,Villarreal C,Tyner C,Speir ML,Rosenbloom KR,Raney BJ,Lee CM,Lee BT,Karolchik D,Hinrichs AS,Haeussler M,Guruvadoo L,Navarro Gonzalez J,Gibson D,Fiddes IT,Eisenhart C,Diekhans M,Clawson H,Barber GP,

更新日期：2018-01-04 00:00:00

abstract：:The CDC16 gene is involved in the process of chromosome segregation in mitosis and a cdc16ts mutant accumulates the predominant microtubule-associated protein at the nonpermissive temperature. We find that the CDC16 gene open reading frame (ORF) is capable of encoding a protein whose calculated molecular weight and pI...

journal_title：Nucleic acids research

authors： Icho T,Wickner RB

更新日期：1987-10-26 00:00:00

abstract：:N-nitrosation of glycine and its derivatives generates potent alkylating agents that can lead to the formation of O(6)-carboxymethylguanine (O(6)-CMG) in DNA. O(6)-CMG has been identified in DNA derived from human colon tissue, and its occurrence has been linked to diets high in red and processed meats. By analogy to ...

journal_title：Nucleic acids research

authors： Zhang F,Tsunoda M,Suzuki K,Kikuchi Y,Wilkinson O,Millington CL,Margison GP,Williams DM,Czarina Morishita E,Takénaka A

更新日期：2013-05-01 00:00:00

abstract：:ADGO 2.0 is a web-based tool that provides composite interpretations for microarray data comparing two sample groups as well as lists of genes from diverse sources of biological information. Some other tools also incorporate composite annotations solely for interpreting lists of genes but usually provide highly redund...

journal_title：Nucleic acids research

authors： Chi SM,Kim J,Kim SY,Nam D

更新日期：2011-07-01 00:00:00

abstract：:The origin of replication of the c-myc gene in HeLa cells was previously identified at low resolution within 3.5 kb 5' to the P1 promoter, based on replication fork polarity and the location of DNA nascent strands. To define the initiation events in the c-myc origin at higher resolution the template bias of nascent DN...

journal_title：Nucleic acids research

authors： Waltz SE,Trivedi AA,Leffak M

更新日期：1996-05-15 00:00:00

abstract：:Interest in secondary metabolites such as RiPPs (ribosomally synthesized and posttranslationally modified peptides) is increasing worldwide. To facilitate the research in this field we have updated our mining web server. BAGEL4 is faster than its predecessor and is now fully independent from ORF-calling. Gene clusters...

journal_title：Nucleic acids research

authors： van Heel AJ,de Jong A,Song C,Viel JH,Kok J,Kuipers OP

更新日期：2018-07-02 00:00:00

abstract：:We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process was mediated by a s...

journal_title：Nucleic acids research

authors： Yu BJ,Kang KH,Lee JH,Sung BH,Kim MS,Kim SC

更新日期：2008-08-01 00:00:00

abstract：:The chromatographic profiles of isoaccepting tRNAs were analyzed at five time points during the 96 hr, dimethylsulfoxide induced, erythroid-like differentiation of Friend leukemia cells. Sixty-four isoaccepting species of tRNA for 16 amino acids were resolved by RPC-5 chromatography. The relative amounts of tRNAphe, t...

journal_title：Nucleic acids research

authors： Lin VK,Agris PF

更新日期：1980-08-11 00:00:00

abstract：:In general, synthetic RNA transcripts corresponding to the 3' ends of Saccharomyces cerevisiae genes appear to be accurately cleaved and polyadenylated in vitro under appropriate conditions in yeast cell extracts. Initially, however, the endpoints observed in vitro for the GAL7 gene failed to correlate adequately with...

journal_title：Nucleic acids research

authors： Sadhale PP,Sapolsky R,Davis RW,Butler JS,Platt T

更新日期：1991-07-11 00:00:00

abstract：:Humans have two nearly identical copies of the survival motor neuron (SMN ) gene, SMN1 and SMN2. Homozygous loss of SMN1 causes spinal muscular atrophy (SMA). SMN2 is unable to prevent the disease due to skipping of exon 7. Using a systematic approach of in vivo selection, we have previously demonstrated that a weak 5...

journal_title：Nucleic acids research

authors： Singh NN,Singh RN,Androphy EJ

更新日期：2007-01-01 00:00:00

abstract：:Loss-of-function in melanocortin 1 receptor (MC1R), a GS protein-coupled receptor that regulates signal transduction through cAMP and protein kinase A (PKA) in melanocytes, is a major inherited melanoma risk factor. Herein, we report a novel cAMP-mediated response for sensing and responding to UV-induced DNA damage re...

journal_title：Nucleic acids research

authors： Jarrett SG,Wolf Horrell EM,D'Orazio JA

更新日期：2016-12-15 00:00:00

abstract：:DNA synthesis is a fundamental requirement for cell proliferation and DNA repair, but no single method can identify the location, direction and speed of replication forks with high resolution. Mammalian cells have the ability to incorporate thymidine analogs along with the natural A, T, G and C bases during DNA synthe...

journal_title：Nucleic acids research

authors： Georgieva D,Liu Q,Wang K,Egli D

更新日期：2020-09-04 00:00:00

abstract：:Comparisons of proteins show that they evolve through the movement of domains. However, in many cases, the underlying mechanisms remain unclear. Here, we observed the movements of DNA recognition domains between non-orthologous proteins within a prokaryote genome. Restriction-modification (RM) systems, consisting of a...

journal_title：Nucleic acids research

authors： Furuta Y,Kobayashi I

更新日期：2012-10-01 00:00:00

abstract：:The integrity and stability of nucleosomes under transcription assay conditions has been found to depend on concentration and ionic environment. Rifamycin AF/013, a commonly used inhibitor of initiation, is particularly effective in destabilisation of nucleosomes. Intact nucleosomes are refractory to transcription by ...

journal_title：Nucleic acids research

authors： Lilley DM,Jacobs MF,Houghton M

更新日期：1979-09-25 00:00:00

abstract：:Insertion of the insertion sequence Is2(I) directly before the galE gene of the galactose operon results in a Gal minus phenotype (1, 2). The Gal-constitutive allele galc200 (and its deletion derivative galc200 delta 31) arise from such a Gal minus mutant by the insertion of LS2(II) DNA within the LS2(I) sequence (3)....

journal_title：Nucleic acids research

authors： Hinton DM,Musso RE

更新日期：1982-08-25 00:00:00

abstract：:Alu elements have repeatedly been found involved in gene rearrangements in humans. Although these elements have been suggested to stimulate gene rearrangements, sparse information is available for the possible mechanism(s) of these events. Here we present a compilation of Alu elements that have been involved in recomb...

journal_title：Nucleic acids research

authors： Rüdiger NS,Gregersen N,Kielland-Brandt MC

更新日期：1995-01-25 00:00:00

abstract：:The binding of transcription factors to specific regulatory sequence elements is a primary mechanism for controlling gene transcription. Eukaryotic genes are often regulated by several transcription factors whose binding sites are tightly clustered and form cis-regulatory modules. In this paper, we present a web serve...

journal_title：Nucleic acids research

authors： Sharan R,Ben-Hur A,Loots GG,Ovcharenko I

更新日期：2004-07-01 00:00:00

abstract：:With the improved secreted protein prediction approach and comprehensive data sources, including Swiss-Prot, TrEMBL, RefSeq, Ensembl and CBI-Gene, we have constructed secretomes of human, mouse and rat, with a total of 18 152 secreted proteins. All the entries are ranked according to the prediction confidence. They we...

journal_title：Nucleic acids research

authors： Chen Y,Zhang Y,Yin Y,Gao G,Li S,Jiang Y,Gu X,Luo J

更新日期：2005-01-01 00:00:00

abstract：:The VISTA portal for comparative genomics is designed to give biomedical scientists a unified set of tools to lead them from the raw DNA sequences through the alignment and annotation to the visualization of the results. The VISTA portal also hosts the alignments of a number of genomes computed by our group, allowing ...

journal_title：Nucleic acids research

authors： Brudno M,Poliakov A,Minovitsky S,Ratnere I,Dubchak I

更新日期：2007-07-01 00:00:00