Abstract:
:The integrity and stability of nucleosomes under transcription assay conditions has been found to depend on concentration and ionic environment. Rifamycin AF/013, a commonly used inhibitor of initiation, is particularly effective in destabilisation of nucleosomes. Intact nucleosomes are refractory to transcription by wheat RNA polymerase II, the histone core preventing initiation. Template titration suggests that the polymerase can, however, bind to nucleosomes, and a 15--16S complex has been observed on sucrose gradients. DNase I digestion of polymerase-nucleosome incubations indicates that whilst histone is still present in the complex, the nucleosome conformation is altered resulting in enhanced nucleolysis at sites near the DNA centre but reduced overall kinetics of digestion.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Lilley DM,Jacobs MF,Houghton Mdoi
10.1093/nar/7.2.377subject
Has Abstractpub_date
1979-09-25 00:00:00pages
377-99issue
2eissn
0305-1048issn
1362-4962journal_volume
7pub_type
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1990-07-11 00:00:00
abstract::The nucleotide sequence of the control region of the divergent argECBH operon has been established in the wild type and in mutants affecting expression of these genes. The argE and argCBH promoters face each other and overlap with an operator region containing two domains which may act as distinct repressor binding si...
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Nucleic acids research
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pub_type: 杂志文章
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abstract::RNA-protein complexes are essential in mediating important fundamental cellular processes, such as transport and localization. In particular, ncRNA-protein interactions play an important role in post-transcriptional gene regulation like mRNA localization, mRNA stabilization, poly-adenylation, splicing and translation....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv020
更新日期:2015-02-18 00:00:00
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journal_title:Nucleic acids research
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abstract::Single-strand specific endoribonuclease YbeY has been shown to play an important role in the processing of the 3' end of the 16S rRNA in Escherichia coli. Lack of YbeY results in the accumulation of the 17S rRNA precursor. In contrast to a previous report, we show that Sinorhizobium meliloti YbeY exhibits endoribonucl...
journal_title:Nucleic acids research
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更新日期:2020-01-10 00:00:00
abstract::Several mono-, di-, tetra-, penta- and nonaribozymes were developed. These multitarget-ribozymes were targeted to cleave HIV-1 env RNA at up to nine different conserved sites. Each multitarget-ribozyme consisted of a chain of up to nine hammerhead motifs, each flanked by a different targeting sequence. The multitarget...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
abstract::Upon infection of Escherichia coli by bacteriophage Qβ, the virus-encoded β-subunit recruits host translation elongation factors EF-Tu and EF-Ts and ribosomal protein S1 to form the Qβ replicase holoenzyme complex, which is responsible for amplifying the Qβ (+)-RNA genome. Here, we use X-ray crystallography, NMR spect...
journal_title:Nucleic acids research
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更新日期:2017-10-13 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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