Determination of DNA cooperativity factor.

Abstract:

:The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5x10(-5) for the cooperatively factor sigma. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Amirikyan BR,Vologodskii AV,Lyubchenko YuL

doi

10.1093/nar/9.20.5469

subject

Has Abstract

pub_date

1981-10-24 00:00:00

pages

5469-82

issue

20

eissn

0305-1048

issn

1362-4962

journal_volume

9

pub_type

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