Abstract:
:The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5x10(-5) for the cooperatively factor sigma. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Amirikyan BR,Vologodskii AV,Lyubchenko YuLdoi
10.1093/nar/9.20.5469subject
Has Abstractpub_date
1981-10-24 00:00:00pages
5469-82issue
20eissn
0305-1048issn
1362-4962journal_volume
9pub_type
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