当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > A site-specific endodeoxyribonuclease from Streptomyces albus CMI 52766 sharing site-specificity with Providencia stuartii endonuclease PstI.

A site-specific endodeoxyribonuclease from Streptomyces albus CMI 52766 sharing site-specificity with Providencia stuartii endonuclease PstI.

Abstract：

:A class II site-specific endodeoxyribonuclease (SalPI) was identified in cell-free extracts of Streptomyces albus CMI 52766 after high speed centrifugation and fractionation through Bio Gel AO.5M. SalPI cleaves lambda DNA into at least 18 fragments. Five cleavage sites were located in the linear lambda map by the use of double and triple restriction enzyme digests involving EcoRI, HindIII, SalGI and another new Streptomyces enzyme, SacI. The results were indistinguishable from those previously obtained for a Providencia stuartii enzyme, PstI, by Smith, Blattner & Davies (Nucleic Acids Res. 1976 3, 343). SalPI and PstI were shown by a double digest test to have the same site specificity. None of 34 phages tested was obviously restricted by S. albus CMI 52766, and correspondingly DNA from two of them was not cleaved in vitro by SalPI. DNA from Streptomyces phage that does not form plaques on S. albus CMI 52766, and plasmid SCP2 DNA from S. coelicolor A3 (2), were both cleaved.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Chater KF

doi

10.1093/nar/4.6.1989

subject

Has Abstract

pub_date

1977-06-01 00:00:00

pages

1989-98

issue

6

eissn

0305-1048

issn

1362-4962

journal_volume

4

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • E1A represses apolipoprotein AI enhancer activity in liver cells through a pRb- and CBP-independent pathway.

    abstract：:The apolipoprotein AI (apoAI) promoter/enhancer contains multiple cis -acting elements on which a variety of hepatocyte-enriched and ubiquitous transcription factors function synergistically to regulate liver-specific transcription. Adenovirus E1A proteins repress tissue-specific gene expression and disrupt the differ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/26.7.1761

    authors： Kilbourne EJ,Evans MJ,Karathanasis SK

    更新日期：1998-04-01 00:00:00

  • Localization of mitochondrial DNA base excision repair to an inner membrane-associated particulate fraction.

    abstract：:Mitochondrial DNA (mtDNA) contains high levels of oxidative damage relative to nuclear DNA. A full, functional DNA base excision repair (BER) pathway is present in mitochondria, to repair oxidative DNA lesions. However, little is known about the organization of this pathway within mitochondria. Here, we provide eviden...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gki683

    authors： Stuart JA,Mayard S,Hashiguchi K,Souza-Pinto NC,Bohr VA

    更新日期：2005-07-08 00:00:00

  • Differences in the phosphate oxygen requirements for self-cleavage by the extended and prototypical hammerhead forms.

    abstract：:The hammerhead self-cleaving motif occurs in a variety of RNAs that infect plants and consists of three non-conserved helices connected by a highly conserved central core. A variant hammerhead, called the extended hammerhead, is found in satellite 2 transcripts from a variety of caudate amphibians. The extended hammer...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/25.11.2189

    authors： Mitrasinovic O,Epstein LM

    更新日期：1997-06-01 00:00:00

  • Streamlining effects of extra telomeric repeat on telomeric DNA folding revealed by fluorescence-force spectroscopy.

    abstract：:A human telomere ends in a single-stranded 3' tail, composed of repeats of T2AG3. G-quadruplexes (GQs) formed from four consecutive repeats have been shown to possess high-structural and mechanical diversity. In principle, a GQ can form from any four repeats that are not necessarily consecutive. To understand the dyna...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkz906

    authors： Mitra J,Ha T

    更新日期：2019-12-02 00:00:00

  • Carboxy terminal modifications of the P0 protein reveal alternative mechanisms of nuclear ribosomal stalk assembly.

    abstract：:The P0 scaffold protein of the ribosomal stalk is mainly incorporated into pre-ribosomes in the cytoplasm where it replaces the assembly factor Mrt4. In analyzing the role of the P0 carboxyl terminal domain (CTD) during ribosomal stalk assembly, we found that its complete removal yields a protein that is functionally ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt637

    authors： Francisco-Velilla R,Remacha M,Ballesta JP

    更新日期：2013-10-01 00:00:00

  • Genome-wide prediction and analysis of human chromatin boundary elements.

    abstract：:Boundary elements partition eukaryotic chromatin into active and repressive domains, and can also block regulatory interactions between domains. Boundary elements act via diverse mechanisms making accurate feature-based computational predictions difficult. Therefore, we developed an unbiased algorithm that predicts th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkr750

    authors： Wang J,Lunyak VV,Jordan IK

    更新日期：2012-01-01 00:00:00

  • DNA synthesis is initiated at two positions within the origin of replication of plasmid R1162.

    abstract：:DNA synthesis of broad host-range plasmid R1162 is initiated from two positions, flanking a large (40 bp stem, 40 bp loop) inverted repeat. Each start-point is located within a highly conserved, but oppositely oriented, 10 base-pair sequence. Synthesis from the two positions converges within the intervening inverted r...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/15.20.8319

    authors： Lin LS,Meyer RJ

    更新日期：1987-10-26 00:00:00

  • Chromosome size polymorphism in Plasmodium falciparum can involve deletions of the subtelomeric pPFrep20 sequence.

    abstract：:The P. falciparum pPFrep20 repetitive element from the Palo Alto Uganda strain has been isolated and sequenced. The Palo Alto pPFrep20 repeat (pPFPArep20) has a clustered subtelomeric location and on chromosome 1 has been deleted from one end. Analysis of chromosome 1 from 5 other strains has revealed that pPFrep20 se...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/16.10.4331

    authors： Patarapotikul J,Langsley G

    更新日期：1988-05-25 00:00:00

  • A simulation of subtractive hybridization.

    abstract：:Various strategies employed in genomic DNA cloning by subtractive hybridization have been examined by computer simulations, with the comparison between the predictions and the published results. The result shows that the efficiency of target sequence enrichment and the sensitivity to experimental conditions depend str...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/26.6.1440

    authors： Cho TJ,Park SS

    更新日期：1998-03-15 00:00:00

  • E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands.

    abstract：:G-quadruplex (G4) DNA structures can form physical barriers within the genome that must be unwound to ensure cellular genomic integrity. Here, we report unanticipated roles for the Escherichia coli Rep helicase and RecA recombinase in tolerating toxicity induced by G4-stabilizing ligands in vivo. We demonstrate that R...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa442

    authors： Paul T,Voter AF,Cueny RR,Gavrilov M,Ha T,Keck JL,Myong S

    更新日期：2020-07-09 00:00:00

  • Quality assessment and data analysis for microRNA expression arrays.

    abstract：:MicroRNAs are small (approximately 22 nt) RNAs that regulate gene expression and play important roles in both normal and disease physiology. The use of microarrays for global characterization of microRNA expression is becoming increasingly popular and has the potential to be a widely used and valuable research tool. H...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkn932

    authors： Sarkar D,Parkin R,Wyman S,Bendoraite A,Sather C,Delrow J,Godwin AK,Drescher C,Huber W,Gentleman R,Tewari M

    更新日期：2009-02-01 00:00:00

  • Complete nucleotide sequence of the Escherichia coli recB gene.

    abstract：:The complete nucleotide sequence of the Escherichia coli recB gene which encodes a subunit of the ATP-dependent DNase, Exonuclease V, has been determined. The proposed coding region for the RecB protein is 3543 nucleotides long and would encode a polypeptide of 1180 amino acids with a calculated molecular weight of 13...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/14.21.8573

    authors： Finch PW,Storey A,Chapman KE,Brown K,Hickson ID,Emmerson PT

    更新日期：1986-11-11 00:00:00

  • Conservation, localization and expression of HopZ, a protein involved in adhesion of Helicobacter pylori.

    abstract：:From a sarkosyl-insoluble outer membrane fraction prepared from the Helicobacter pylori strain ATCC 43504, 19 proteins could be sequenced N-terminally by Edman degradation. Oligonucleotides were deduced and used for screening of a genomic library. From the isolated genes, five code for different members of a H.pylori ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/27.16.3325

    authors： Peck B,Ortkamp M,Diehl KD,Hundt E,Knapp B

    更新日期：1999-08-15 00:00:00

  • Nucleolar proteins Bfr2 and Enp2 interact with DEAD-box RNA helicase Dbp4 in two different complexes.

    abstract：:Different pre-ribosomal complexes are formed during ribosome biogenesis, and the composition of these complexes is highly dynamic. Dbp4, a conserved DEAD-box RNA helicase implicated in ribosome biogenesis, interacts with nucleolar proteins Bfr2 and Enp2. We show that, like Dbp4, Bfr2 and Enp2 are required for the earl...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1293

    authors： Soltanieh S,Lapensée M,Dragon F

    更新日期：2014-03-01 00:00:00

  • The internal region of CtIP negatively regulates DNA end resection.

    abstract：:DNA double-strand breaks are repaired by end-joining or homologous recombination. A key-committing step of recombination is DNA end resection. In resection, phosphorylated CtIP first promotes the endonuclease of MRE11-RAD50-NBS1 (MRN). Subsequently, CtIP also stimulates the WRN/BLM-DNA2 pathway, coordinating thus both...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa273

    authors： Howard SM,Ceppi I,Anand R,Geiger R,Cejka P

    更新日期：2020-06-04 00:00:00

  • The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129.

    abstract：:Corynebacterium diphtheriae is a Gram-positive, non-spore forming, non-motile, pleomorphic rod belonging to the genus Corynebacterium and the actinomycete group of organisms. The organism produces a potent bacteriophage-encoded protein exotoxin, diphtheria toxin (DT), which causes the symptoms of diphtheria. This pote...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg874

    authors： Cerdeño-Tárraga AM,Efstratiou A,Dover LG,Holden MT,Pallen M,Bentley SD,Besra GS,Churcher C,James KD,De Zoysa A,Chillingworth T,Cronin A,Dowd L,Feltwell T,Hamlin N,Holroyd S,Jagels K,Moule S,Quail MA,Rabbinowitsch E

    更新日期：2003-11-15 00:00:00

  • The two Saccharomyces cerevisiae SUA7 (TFIIB) transcripts differ at the 3'-end and respond differently to stress.

    abstract：:Despite much information as to the structure and function of the general transcription factors, little is known about the regulation of their expression. Transcription of the Saccharomyces cerevisiae SUA7 (TFIIB) gene results in the formation of two discrete transcripts. It was originally reported that the two transcr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/28.22.4435

    authors： Hoopes BC,Bowers GD,DiVisconte MJ

    更新日期：2000-11-15 00:00:00

  • The MH1 domain of Smad3 interacts with Pax6 and represses autoregulation of the Pax6 P1 promoter.

    abstract：:Pax6 transcription is under the control of two main promoters (P0 and P1), and these are autoregulated by Pax6. Additionally, Pax6 expression is under the control of the TGFbeta superfamily, although the precise mechanisms of such regulation are not understood. The effect of TGFbeta on Pax6 expression was studied in t...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkl1105

    authors： Grocott T,Frost V,Maillard M,Johansen T,Wheeler GN,Dawes LJ,Wormstone IM,Chantry A

    更新日期：2007-01-01 00:00:00

  • Gene expression atlas at the European bioinformatics institute.

    abstract：:The Gene Expression Atlas (http://www.ebi.ac.uk/gxa) is an added-value database providing information about gene expression in different cell types, organism parts, developmental stages, disease states, sample treatments and other biological/experimental conditions. The content of this database derives from curation, ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkp936

    authors： Kapushesky M,Emam I,Holloway E,Kurnosov P,Zorin A,Malone J,Rustici G,Williams E,Parkinson H,Brazma A

    更新日期：2010-01-01 00:00:00

  • Phosphorylation of human oxoguanine DNA glycosylase (alpha-OGG1) modulates its function.

    abstract：:Oxoguanine DNA glycosylase (OGG1) initiates the repair of 8-oxoguanine (8-oxoG), a major oxidative DNA base modification that has been directly implicated in cancer and aging. OGG1 functions in the base excision repair pathway, for which a molecular hand-off mechanism has been proposed. To date, only one functional an...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gki636

    authors： Hu J,Imam SZ,Hashiguchi K,de Souza-Pinto NC,Bohr VA

    更新日期：2005-06-07 00:00:00

  • DNA repair of pyrimidine dimers and 6-4 photoproducts in the ribosomal DNA.

    abstract：:The nucleolus is a unique structural component of interphase nuclei where the ribosomal genes, trans-cribed by RNA polymerase I (RNA pol I), are organized. In the present study, the repair of UV-induced photolesions was investigated in the ribosomal DNA (rDNA) in relation to RNA pol I transcription. We used hamster ce...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/27.12.2511

    authors： Balajee AS,May A,Bohr VA

    更新日期：1999-06-15 00:00:00

  • The evolution of the ribosome biogenesis pathway from a yeast perspective.

    abstract：:Ribosome biogenesis is fundamental for cellular life, but surprisingly little is known about the underlying pathway. In eukaryotes a comprehensive collection of experimentally verified ribosome biogenesis factors (RBFs) exists only for Saccharomyces cerevisiae. Far less is known for other fungi, animals or plants, and...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1137

    authors： Ebersberger I,Simm S,Leisegang MS,Schmitzberger P,Mirus O,von Haeseler A,Bohnsack MT,Schleiff E

    更新日期：2014-02-01 00:00:00

  • The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

    abstract：:The human mitochondrial translation apparatus, which synthesizes the core subunits of the oxidative phosphorylation system, is of central interest as mutations in several genes encoding for mitoribosomal proteins or translation factors cause severe human diseases. Little is known, how this complex machinery assembles ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky701

    authors： Lavdovskaia E,Kolander E,Steube E,Mai MM,Urlaub H,Richter-Dennerlein R

    更新日期：2018-09-19 00:00:00

  • Specific-primer-directed DNA sequencing using automated fluorescence detection.

    abstract：:Automated fluorescence-based DNA sequence analysis offers the possibility to undertake very large scale sequencing projects. Directed strategies, such as the specific-primer-directed sequencing approach ('gene walking'), should prove useful in such projects. Described herein is a study involving the use of this approa...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/17.15.6087

    authors： Kaiser RJ,MacKellar SL,Vinayak RS,Sanders JZ,Saavedra RA,Hood LE

    更新日期：1989-08-11 00:00:00

  • CITED2 silencing sensitizes cancer cells to cisplatin by inhibiting p53 trans-activation and chromatin relaxation on the ERCC1 DNA repair gene.

    abstract：:In this study, we show that silencing of CITED2 using small-hairpin RNA (shCITED2) induced DNA damage and reduction of ERCC1 gene expression in HEK293, HeLa and H1299 cells, even in the absence of cisplatin. In contrast, ectopic expression of ERCC1 significantly reduced intrinsic and induced DNA damage levels, and res...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv934

    authors： Liu YC,Chang PY,Chao CC

    更新日期：2015-12-15 00:00:00

  • The putative promoter of a Xenopus laevis ribosomal gene is reduplicated.

    abstract：:With the aid of a novel poly-dA tailing-partial restriction technique and S1-protection mapping, the 5' terminal coding sequence for the 40S precursor ribosomal RNA of Xenopus laevis has been exactly identified. Since the promoter sequence for the 40S RNA should lie close to its 5' terminal coding sequence, we are abl...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/6.12.3733

    authors： Moss T,Birnstiel ML

    更新日期：1979-08-24 00:00:00

  • A novel strategy for the negative selection in mouse embryonic stem cells operated with immunotoxin-mediated cell targeting.

    abstract：:Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the resp...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/24.18.3653

    authors： Kobayashi K,Ohye T,Pastan I,Nagatsu T

    更新日期：1996-09-15 00:00:00

  • Protein structure prediction using sparse dipolar coupling data.

    abstract：:Residual dipolar coupling (RDC) represents one of the most exciting emerging NMR techniques for protein structure studies. However, solving a protein structure using RDC data alone is still a highly challenging problem. We report here a computer program, RDC-PROSPECT, for protein structure prediction based on a struct...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkh204

    authors： Qu Y,Guo JT,Olman V,Xu Y

    更新日期：2004-01-26 00:00:00

  • Effects of double-strand break repair proteins on vertebrate telomere structure.

    abstract：:Although telomeres are not recognized as double-strand breaks (DSBs), some DSB repair proteins are present at telomeres and are required for telomere maintenance. To learn more about the telomeric function of proteins from the homologous recombination (HR) and non-homologous end joining pathways (NHEJ), we have screen...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkf396

    authors： Wei C,Skopp R,Takata M,Takeda S,Price CM

    更新日期：2002-07-01 00:00:00

  • Long terminal repeat sequences of intracisternal A particle genes in the Syrian hamster genome: identification of tRNAPhe as a putative primer tRNA.

    abstract：:We have determined the nucleotide sequences of long terminal repeat (LTR) regions of Syrian hamster intracisternal A particle (IAP) genes. The size of the LTRs was 350 base-pairs (bp) and 376 bp in two clones, H10 and H18, respectively. Two LTRs at both ends of the IAP gene were linked to directly repeating 6 bp hamst...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/11.20.7169

    authors： Ono M,Ohishi H

    更新日期：1983-10-25 00:00:00