Abstract:
:Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the responsiveness of cells expressing human interleukin-2 receptor alpha subunit to anti-Tac(Fv)-PE40. The immunotoxin treatment efficiently eliminated only ES cells bearing the receptor as a consequence of the target specificity of anti-Tac(Fv)-PE40, indicating that IMCT can be used as a novel strategy for positive and negative selection to enrich ES cell clones with a targeted mutation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kobayashi K,Ohye T,Pastan I,Nagatsu Tdoi
10.1093/nar/24.18.3653subject
Has Abstractpub_date
1996-09-15 00:00:00pages
3653-5issue
18eissn
0305-1048issn
1362-4962pii
l60139journal_volume
24pub_type
杂志文章abstract::Type III restriction enzymes have been demonstrated to require two unmethylated asymmetric recognition sites oriented head-to-head to elicit double-strand break 25-27 bp downstream of one of the two sites. The proposed DNA cleavage mechanism involves ATP-dependent DNA translocation. The sequence context of the recogni...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh899
更新日期:2004-10-22 00:00:00
abstract::Eukaryotic High-Mobility Group B (HMGB) proteins alter DNA elasticity while facilitating transcription, replication and DNA repair. We developed a new single-molecule method to probe non-specific DNA interactions for two HMGB homologs: the human HMGB2 box A domain and yeast Nhp6Ap, along with chimeric mutants replacin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1031
更新日期:2013-01-07 00:00:00
abstract::The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other h...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.24.8211
更新日期:1982-12-20 00:00:00
abstract::Deproteinated 16S RNA was iodinated at pH 5.0 in an aqueous solution containing TlCl3 plus KI for 1-5 hours at 42 degrees C. Under these conditions 33 moles of iodine are incorporated per mole of RNA. As judged by sucrose gradient sedimentation, the iodinated RNA does not exhibit any large alteration in conformation a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/3.11.3001
更新日期:1976-11-01 00:00:00
abstract::Recurrent chromosome aberrations are an important resource when associating human pathologies to specific genes. However, for technical reasons a large number of chromosome breakpoints are defined only at the level of cytobands and many of the genes involved remain unidentified. We developed a web-based information sy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki061
更新日期:2005-01-01 00:00:00
abstract::Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1034
更新日期:2016-02-18 00:00:00
abstract::PrankWeb is an online resource providing an interface to P2Rank, a state-of-the-art method for ligand binding site prediction. P2Rank is a template-free machine learning method based on the prediction of local chemical neighborhood ligandability centered on points placed on a solvent-accessible protein surface. Points...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz424
更新日期:2019-07-02 00:00:00
abstract::Motif3D is a web-based protein structure viewer designed to allow sequence motifs, and in particular those contained in the fingerprints of the PRINTS database, to be visualised on three-dimensional (3D) structures. Additional functionality is provided for the rhodopsin-like G protein-coupled receptors, enabling finge...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg534
更新日期:2003-07-01 00:00:00
abstract::In silico screening based on the structures of the ligands or of the receptors has become an essential tool to facilitate the drug discovery process but compound collections are needed to carry out such in silico experiments. It has been recognized that absorption, distribution, metabolism, excretion and toxicity (ADM...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl065
更新日期:2006-07-01 00:00:00
abstract::To investigate the feasibility of conducting a genomic-scale protein labeling and localization study in Escherichia coli, a representative subset of 23 coding DNA sequences (CDSs) was selected for chromosomal tagging with one or more fluorescent protein genes (EGFP, EYFP, mRFP1, DsRed2). We used lambda-Red recombinati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1158
更新日期:2007-01-01 00:00:00
abstract::RNA polymerase I-catalyzed synthesis of the Schizosaccharomyces pombe approximately 37S pre-rRNAs was shown to be sensitive to regulatory sequences located several kilobases upstream of the initiation site for the rRNA gene. An in vitro transcription system for RNA polymerase I-catalyzed RNA synthesis was established ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.3.659
更新日期:1997-02-01 00:00:00
abstract::The HIRA histone chaperone complex deposits histone H3.3 into nucleosomes in a DNA replication- and sequence-independent manner. As herpesvirus genomes enter the nucleus as naked DNA, we asked whether the HIRA chaperone complex affects herpesvirus infection. After infection of primary cells with HSV or CMV, or transie...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx771
更新日期:2017-11-16 00:00:00
abstract::The molecular mechanism in pancreatic β cells underlying hyperlipidemia and insulin insufficiency remains unclear. Here, we find that the fatty acid-induced decrease in insulin levels occurs due to a decrease in insulin translation. Since regulation at the translational level is generally mediated through RNA-binding ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky867
更新日期:2018-12-14 00:00:00
abstract::The kinetics of PaeR7 endonuclease-catalysed cleavage reactions of fluorophor-labeled oligonucleotide substrates have been examined using fluorescence resonance energy transfer (FRET). A series of duplex substrates were synthesized with an internal CTCGAG PaeR7 recognition site and donor (fluorescein) and acceptor (rh...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.15.3155
更新日期:1994-08-11 00:00:00
abstract::The Escherichia coli RuvC protein is an endonuclease that resolves Holliday junctions. In vitro, the protein shows efficient structure-specific binding of Holliday junctions, yet the rate of junction resolution is remarkably low. We have mapped the sites of cleavage on a synthetic junction through which a crossover ca...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.13.2490
更新日期:1994-07-11 00:00:00
abstract::We present the results of in vitro binding studies aimed at defining the key recognition elements on the MS2 RNA translational operator (TR) essential for complex formation with coat protein. We have used chemically synthesized operators carrying modified functional groups at defined nucleotide positions, which are es...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.13.2512
更新日期:1995-07-11 00:00:00
abstract::ADAR enzymes, adenosine deaminases that act on RNA, form a family of RNA editing enzymes that convert adenosine to inosine within RNA that is completely or largely double-stranded. Site-selective A-->I editing has been detected at specific sites within a few structured pre-mRNAs of metazoans. We have analyzed the edit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg681
更新日期:2003-08-15 00:00:00
abstract::Primer Design Assistant (PDA) is a web interface primer design service combined with thermodynamic theory to evaluate the fitness of primers. It runs in a Linux-Apache-MySQL-PHP structure on a PC equipped with dual CPU (Intel Pentium III 1.4 GHz) and 512 Mb of RAM. A succinct user interface of PDA is accomplished by b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg560
更新日期:2003-07-01 00:00:00
abstract::A method for the facile preparation of oligoribonucleotide analogues containing beta-d-allofuranosyl nucleosides with additional functional groups tethered to the 6'-O positions is presented. It is based on the synthesis of two protected nucleosides carrying a 6'-O -bromopentyl and a 6'-O -methylaminopentyl substituen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.19.4315
更新日期:1998-10-01 00:00:00
abstract::A key issue when designing and using DNA-targeting nucleases is specificity. Ideally, an optimal DNA-targeting tool has only one recognition site within a genomic sequence. In practice, however, almost all designer nucleases available today can accommodate one to several mutations within their target site. The ability...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku155
更新日期:2014-04-01 00:00:00
abstract::PubChem (http://pubchem.ncbi.nlm.nih.gov) is a public repository for biological activity data of small molecules and RNAi reagents. The mission of PubChem is to deliver free and easy access to all deposited data, and to provide intuitive data analysis tools. The PubChem BioAssay database currently contains 500,000 des...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1132
更新日期:2012-01-01 00:00:00
abstract::Enzymes involved in nucleic acid transactions often have a helicase-like ATPase coordinating and driving their functional activities, but our understanding of the mechanistic details of their coordination is limited. For example, DNA cleavage by the antiphage defense system Type ISP restriction-modification enzyme req...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa023
更新日期:2020-03-18 00:00:00
abstract::The binary Cre-lox conditional knockout system requires an essential part of the target gene to be flanked by loxP sites, enabling excision in vivo upon Cre expression. LoxP sites are introduced by homologous recombination, together with a selectable marker. However, this marker can disturb gene expression and should ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng021
更新日期:2003-03-01 00:00:00
abstract::Pds5 is required for sister chromatid cohesion, and somewhat paradoxically, to remove cohesin from chromosomes. We found that Pds5 plays a critical role during DNA replication that is distinct from its previously known functions. Loss of Pds5 hinders replication fork progression in unperturbed human and mouse cells. I...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky519
更新日期:2019-02-20 00:00:00
abstract::Modified nucleosides in natural RNA molecules are essential for their functions. Non-natural nucleoside analogues have been introduced into RNA to manipulate its structure and function. We have recently developed a new strategy for the in situ modification of RNA based on the functionality transfer reaction between an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku538
更新日期:2014-07-01 00:00:00
abstract::KUPS (The University of Kansas Proteomics Service) provides high-quality protein-protein interaction (PPI) data for researchers developing and evaluating computational models for predicting PPIs by allowing users to construct ready-to-use data sets of interacting protein pairs (IPPs), non-interacting protein pairs (NI...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq943
更新日期:2011-01-01 00:00:00
abstract::During the splicing process, spliceosomal snRNAs undergo numerous conformational rearrangements that appear to be catalyzed by proteins belonging to the DEAD/H-box superfamily of RNA helicases. We have cloned a new RNA helicase gene, designated DBP2 (DEAH-boxprotein), homologous to the Schizosaccaromyces pombe cdc28(+...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.9.2063
更新日期:1998-05-01 00:00:00
abstract::The specific recognition of homopurine-homo pyrimidine regions in duplex DNA by triplex-forming oligonucleotides (TFOs) provides an attractive strategy for genetic manipulation. Alkylation of nucleobases with functionalized TFOs would have the potential for site-directed mutagenesis. Recently, we demonstrated that a T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gng031
更新日期:2003-03-15 00:00:00
abstract::We report here the construction of engineered endonuclease database (EENdb) (http://eendb.zfgenetics.org/), a searchable database and knowledge base for customizable engineered endonucleases (EENs), including zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). EENs are artificial...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1144
更新日期:2013-01-01 00:00:00
abstract::The mRNAs for different variant surface antigens of Trypanosoma brucei start with the same 35 nucleotides. This sequence is encoded by a separate mini-exon, located in a 1.35-kb repetitive element. We have reported that trypanosomes contain many transcripts that hybridize to mini-exon probes, even if they do not make ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.9.3777
更新日期:1984-05-11 00:00:00