Activation of RuvC Holliday junction resolvase in vitro.

Abstract:

:The Escherichia coli RuvC protein is an endonuclease that resolves Holliday junctions. In vitro, the protein shows efficient structure-specific binding of Holliday junctions, yet the rate of junction resolution is remarkably low. We have mapped the sites of cleavage on a synthetic junction through which a crossover can branch migrate through 26 bp and find that > or = 90% of the junctions were cleaved at one site. This observation of sequence-specific cleavage suggests that inefficient resolution may be due to DNA binding events which occur away from the cleavage site and are therefore non-productive. Holliday junction resolution by RuvC protein can be stimulated by a number of factors including: (i) the presence of Mn2+ (rather than Mg2+) as the divalent metal cofactor, (ii) alkaline pH (< or = 10), and (iii) elevated temperature. These observations may indicate that other proteins are required for efficient RuvC-mediated resolution.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Shah R,Bennett RJ,West SC

doi

10.1093/nar/22.13.2490

subject

Has Abstract

pub_date

1994-07-11 00:00:00

pages

2490-7

issue

13

eissn

0305-1048

issn

1362-4962

journal_volume

22

pub_type

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