Abstract：
:The specific recognition of homopurine-homo pyrimidine regions in duplex DNA by triplex-forming oligonucleotides (TFOs) provides an attractive strategy for genetic manipulation. Alkylation of nucleobases with functionalized TFOs would have the potential for site-directed mutagenesis. Recently, we demonstrated that a TFO bearing 2-amino-6-vinylpurine derivative, 1, achieves triplex-mediated reaction with high selectivity toward the cytosine of the G-C target site. In this report, we have investigated the use of this reagent to target mutations to a specific site in a shuttle vector plasmid, which replicates in mammalian cells. TFOs bearing 1 produced adducts at the complementary position of 1 and thereby introduced mutations at that site during replication/repair of the plasmid in mammalian cells. Reagents that produce covalent cytosine modifications are relatively rare. These TFOs enable the preparation of templates carrying targeted cytosine adducts for in vitro and in vivo studies. The ability to target mutations may prove useful as a tool for studying DNA repair, and as a technique for gene therapy and genetic engineering.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Nagatsugi F,Sasaki S,Miller PS,Seidman MMdoi
10.1093/nar/gng031keywords:
subject
Has Abstractpub_date
2003-03-15 00:00:00pages
e31issue
6eissn
0305-1048issn
1362-4962journal_volume
31pub_type
杂志文章abstract：:We developed a framework for quick and reliable construction of complex gene circuits for genetically engineering mammalian cells. Our hierarchical framework is based on a novel nucleotide addressing system for defining the position of each part in an overall circuit. With this framework, we demonstrate construction o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt605
更新日期：2013-09-01 00:00:00
abstract：:We previously showed that mRNAs synthesized from three genes that naturally lack introns contain a portion of their coding sequence, known as a cytoplasmic accumulation region (CAR), which is essential for stable accumulation of the intronless mRNAs in the cytoplasm. The CAR in each mRNA is unexpectedly large, ranging...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1314
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abstract：:By in vivo DMS footprint and reporter gene analyses we identified two transcription factor binding sites in the human c-sis/PDGF B gene promoter. The low basal activity of the PDGF B promoter in HeLa and undifferentiated K562 cells, which express low PDGF B mRNA levels, and in PC3 cells, which express a high PDGF B mR...
journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.8.1767
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journal_title：Nucleic acids research
pub_type： 杂志文章
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.13.3753
更新日期：1990-07-11 00:00:00
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更新日期：2008-06-01 00:00:00
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更新日期：1993-09-25 00:00:00
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journal_title：Nucleic acids research
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更新日期：1990-06-25 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky813
更新日期：2019-01-08 00:00:00
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks275
更新日期：2012-07-01 00:00:00
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更新日期：1993-05-25 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1303
更新日期：2014-03-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1384
更新日期：2014-04-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.23.9165
更新日期：1984-12-11 00:00:00
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journal_title：Nucleic acids research
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更新日期：1995-06-11 00:00:00
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更新日期：2017-05-05 00:00:00
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journal_title：Nucleic acids research
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更新日期：2013-05-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2003-12-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2017-01-04 00:00:00
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更新日期：1987-04-24 00:00:00
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