The UAS of the yeast PGK gene is composed of multiple functional elements.

abstract：:Trinucleotide repeat (TNR) expansions cause at least 17 heritable neurological diseases, including Huntington's disease. Expansions are thought to arise from abnormal processing of TNR DNA by specific trans-acting proteins. For example, the DNA repair complex MutSβ (MSH2-MSH3 heterodimer) is required in mice for on-go...

journal_title：Nucleic acids research

authors： Gannon AM,Frizzell A,Healy E,Lahue RS

更新日期：2012-11-01 00:00:00

abstract：:Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to ...

journal_title：Nucleic acids research

authors： Al-Hakim AH,Hull R

更新日期：1986-12-22 00:00:00

abstract：:Although telomeres are not recognized as double-strand breaks (DSBs), some DSB repair proteins are present at telomeres and are required for telomere maintenance. To learn more about the telomeric function of proteins from the homologous recombination (HR) and non-homologous end joining pathways (NHEJ), we have screen...

journal_title：Nucleic acids research

authors： Wei C,Skopp R,Takata M,Takeda S,Price CM

更新日期：2002-07-01 00:00:00

abstract：:Slime mold, plant and insect dihydropyrimidine amidohydrolases (DHPases, EC 3.5.2.2), which catalyze the second step of pyrimidine and several anti-cancer drug degradations, were cloned and shown to functionally replace a defective DHPase enzyme in the yeast Saccharomyces kluyveri. The yeast and slime mold DHPases wer...

journal_title：Nucleic acids research

authors： Gojkovic Z,Rislund L,Andersen B,Sandrini MP,Cook PF,Schnackerz KD,Piskur J

更新日期：2003-03-15 00:00:00

abstract：:Understanding networks of protein-protein interactions constitutes an essential component on a path towards comprehensive description of cell function. Whereas efficient techniques are readily available for the initial identification of interacting protein partners, practical strategies are lacking for the subsequent ...

journal_title：Nucleic acids research

authors： Pajunen M,Turakainen H,Poussu E,Peränen J,Vihinen M,Savilahti H

更新日期：2007-01-01 00:00:00

abstract：:The distribution patterns of shared short repetitive motifs in the expansion segments of the large subunit rRNA genes of different species show that these segments are coevolving as a set and that in two examined vertebrate species the RNA secondary structures are conserved as a consequence of runs of motifs in one re...

journal_title：Nucleic acids research

authors： Hancock JM,Dover GA

更新日期：1990-10-25 00:00:00

abstract：:Replacement of alpha-, beta- and gamma-phosphate groups in 2'-deoxynucleoside 5'-triphosphates (dNTP) with phosphonate groups yields a new set of dNTP mimics with potential biological and therapeutic applications. Here, we describe the synthesis of 15 new dNTPs modified at alpha-, beta- and gamma-phosphates containing...

journal_title：Nucleic acids research

authors： Alexandrova LA,Skoblov AY,Jasko MV,Victorova LS,Krayevsky AA

更新日期：1998-02-01 00:00:00

abstract：:A soluble cell-free extract containing RNA polymerase III and factors essential for selective transcription of the yeast SUP4-o tRNATyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA polymerase III was identified within the yeast tRNATyr coding sequence by testing several s...

journal_title：Nucleic acids research

authors： Koski RA,Allison DS,Worthington M,Hall BD

更新日期：1982-12-20 00:00:00

abstract：:N-nitrosation of glycine and its derivatives generates potent alkylating agents that can lead to the formation of O(6)-carboxymethylguanine (O(6)-CMG) in DNA. O(6)-CMG has been identified in DNA derived from human colon tissue, and its occurrence has been linked to diets high in red and processed meats. By analogy to ...

journal_title：Nucleic acids research

authors： Zhang F,Tsunoda M,Suzuki K,Kikuchi Y,Wilkinson O,Millington CL,Margison GP,Williams DM,Czarina Morishita E,Takénaka A

更新日期：2013-05-01 00:00:00

abstract：:AvrBs3, the founding member of the Xanthomonas transcription-activator-like effectors (TALEs), is translocated into the plant cell where it localizes to the nucleus and acts as transcription factor. The DNA-binding domain of AvrBs3 consists of 17.5 nearly-identical 34 amino acid-repeats. Each repeat specifies binding ...

journal_title：Nucleic acids research

authors： Schreiber T,Bonas U

更新日期：2014-06-01 00:00:00

abstract：:The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted cod...

journal_title：Nucleic acids research

authors： Azuma Y,Yamagishi M,Ueshima R,Ishihama A

更新日期：1991-02-11 00:00:00

abstract：:IGF2 mRNA-binding protein 1 (IMP1) is a key regulator of messenger RNA (mRNA) metabolism and transport in organismal development and, in cancer, its mis-regulation is an important component of tumour metastasis. IMP1 function relies on the recognition of a diverse set of mRNA targets that is mediated by the combinator...

journal_title：Nucleic acids research

authors： Dagil R,Ball NJ,Ogrodowicz RW,Hobor F,Purkiss AG,Kelly G,Martin SR,Taylor IA,Ramos A

更新日期：2019-05-07 00:00:00

abstract：:The nucleotide sequence of a 981 bp's HincII-PvuII DNA fragment containing the 3' end of the malEFG operon in E. coli was determined. This sequence displayed a putative Rho-independent transcription termination site localized 87 bp's after the stop codon of malG. When cloned into plasmid pKG1800, the HincII-PvuII frag...

journal_title：Nucleic acids research

authors： Francoz E,Dassa E

更新日期：1988-05-11 00:00:00

abstract：:Microarrays are an effective tool for monitoring genome-wide gene expression levels. In current microarray analyses, the majority of genes on arrays are frequently eliminated for further analysis because the changes in their expression levels (ratios) are considered to be not significant. This strategy risks failure t...

journal_title：Nucleic acids research

authors： Yano K,Imai K,Shimizu A,Hanashita T

更新日期：2006-03-14 00:00:00

abstract：:A sensitive gel retention assay has been utilized to detect proteins from uninfected Hela nuclei which interact with the adenovirus type 2 enhancer. This assay has been employed to monitor fractionation of nuclear extracts. Three enhancer binding factors were resolved by chromatography on DEAE-Sepharose and one of the...

journal_title：Nucleic acids research

authors： Barrett P,Clark L,Hay RT

更新日期：1987-03-25 00:00:00

abstract：:HotSpot Wizard is a web server used for the automated identification of hotspots in semi-rational protein design to give improved protein stability, catalytic activity, substrate specificity and enantioselectivity. Since there are three orders of magnitude fewer protein structures than sequences in bioinformatic datab...

journal_title：Nucleic acids research

authors： Sumbalova L,Stourac J,Martinek T,Bednar D,Damborsky J

更新日期：2018-07-02 00:00:00

abstract：:Comparative genome hybridization (CGH) to DNA microarrays (array CGH) is a technique capable of detecting deletions and duplications in genomes at high resolution. However, array CGH studies of the human genome noting false negative and false positive results using large insert clones as probes have raised important c...

journal_title：Nucleic acids research

authors： Price TS,Regan R,Mott R,Hedman A,Honey B,Daniels RJ,Smith L,Greenfield A,Tiganescu A,Buckle V,Ventress N,Ayyub H,Salhan A,Pedraza-Diaz S,Broxholme J,Ragoussis J,Higgs DR,Flint J,Knight SJ

更新日期：2005-06-16 00:00:00

abstract：:We have compiled the DNA sequence data for E. coli K12 available from the GENBANK and EMBO databases and over a period of several years independently from the literature. We have introduced all available genetic map data and have arranged the sequences accordingly. As far as possible the overlaps are deleted and a tot...

journal_title：Nucleic acids research

authors： Kröger M

更新日期：1989-01-01 00:00:00

abstract：:The R-Coffee web server produces highly accurate multiple alignments of noncoding RNA (ncRNA) sequences, taking into account predicted secondary structures. R-Coffee uses a novel algorithm recently incorporated in the T-Coffee package. R-Coffee works along the same lines as T-Coffee: it uses pairwise or multiple seque...

journal_title：Nucleic acids research

authors： Moretti S,Wilm A,Higgins DG,Xenarios I,Notredame C

更新日期：2008-07-01 00:00:00

abstract：:Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguan...

journal_title：Nucleic acids research

authors： Agris PF,Schmidt PG

更新日期：1980-05-10 00:00:00

abstract：:Signals setting the level of transcription of the tRNA-tufB operon have been studied by deletion mapping. TufB transcription was measured in vivo with plasmid-borne tRNA-tufB:galk operon fusions. Removal of the sequences from -133 to -58 with respect to the transcription start point, results in a 90% decrease of tufB ...

journal_title：Nucleic acids research

authors： van Delft JH,Mariñon B,Schmidt DS,Bosch L

更新日期：1987-11-25 00:00:00

abstract：:DNA breaks occur during many processes in mammalian cells, including recombination, repair, mutagenesis and apoptosis. Here we report a simple and rapid method for assaying DNA breaks and identifying DNA breaksites. Breaksites are first tagged and amplified by ligation-mediated PCR (LM-PCR), using nested PCR primers t...

journal_title：Nucleic acids research

authors： Kong Q,Maizels N

更新日期：2001-03-15 00:00:00

abstract：:Melibiose uptake and hydrolysis in E.coli is performed by the MelB and MelA proteins, respectively. We report the cloning and sequencing of the melA gene. The nucleotide sequence data showed that melA codes for a 450 amino acid long protein with a molecular weight of 50.6 kd. The sequence data also supported the assum...

journal_title：Nucleic acids research

authors： Liljeström PL,Liljeström P

更新日期：1987-03-11 00:00:00

abstract：:Restarting stalled replication forks is vital to avoid fatal replication errors. Previously, it was demonstrated that hydroxyurea-stalled replication forks rescue replication either by an active restart mechanism or by new origin firing. To our surprise, using the DNA fibre assay, we only detect a slightly reduced for...

journal_title：Nucleic acids research

authors： Elvers I,Johansson F,Groth P,Erixon K,Helleday T

更新日期：2011-09-01 00:00:00

abstract：:MicroRNAs are small non-coding RNAs that modulate gene expression at post-transcriptional level, playing a crucial role in cell differentiation and development. Recently, some reports have shown that a limited number of mammalian microRNAs are also involved in anti-viral defense. In this study, the analysis of the hep...

journal_title：Nucleic acids research

authors： Potenza N,Papa U,Mosca N,Zerbini F,Nobile V,Russo A

更新日期：2011-07-01 00:00:00

abstract：:Yeast uses nucleotide excision repair (NER) and photolyase (photoreactivation) to repair cyclobutane pyrimidine dimers (CPDs) generated by ultraviolet light. In active genes, NER preferentially repairs the transcribed strand (TS). In contrast, we recently showed that photolyase preferentially repairs the non-transcrib...

journal_title：Nucleic acids research

authors： Livingstone-Zatchej M,Meier A,Suter B,Thoma F

更新日期：1997-10-01 00:00:00

abstract：:The genetic etiology of premature ovarian insufficiency (POI) has been well established to date, however, the role of long noncoding RNAs (lncRNAs) in POI is largely unknown. In this study, we identified a down-expressed lncRNA HCP5 in granulosa cells (GCs) from biochemical POI (bPOI) patients, which impaired DNA dama...

journal_title：Nucleic acids research

authors： Wang X,Zhang X,Dang Y,Li D,Lu G,Chan WY,Leung PCK,Zhao S,Qin Y,Chen ZJ

更新日期：2020-05-07 00:00:00

abstract：:The transcription factor Sox5 has previously been shown in chicken to be expressed in early neural crest cells and neural crest-derived peripheral glia. Here, we show in mouse that Sox5 expression also continues after neural crest specification in the melanocyte lineage. Despite its continued expression, Sox5 has litt...

journal_title：Nucleic acids research

authors： Stolt CC,Lommes P,Hillgärtner S,Wegner M

更新日期：2008-10-01 00:00:00

abstract：:Mitochondrial transcription factor A (TFAM) stimulates transcription from mitochondrial DNA (mtDNA) promoters in vitro and in organello. To investigate whether changes of TFAM levels also modulate transcription and replication in situ, the protein was transiently overexpressed in cultured cells. Mitochondrial mRNAs we...

journal_title：Nucleic acids research

authors： Maniura-Weber K,Goffart S,Garstka HL,Montoya J,Wiesner RJ

更新日期：2004-11-16 00:00:00

abstract：:Proteins that catalyse homologous recombination have been identified in all living organisms and are essential for the repair of damaged DNA as well as for the generation of genetic diversity. In bacteria homologous recombination is performed by the RecA protein, whereas in the eukarya a related protein called Rad51 i...

journal_title：Nucleic acids research

authors： McIlwraith MJ,Hall DR,Stasiak AZ,Stasiak A,Wigley DB,West SC

更新日期：2001-11-15 00:00:00