The folding of the hepatitis C virus internal ribosome entry site depends on the 3'-end of the viral genome.

abstract：:Ten transgenic mouse lines harboring the -346/-103 fragment of the rat insulin I enhancer linked to a heterologous promoter and a reporter gene (Eins-Ptk-CAT construct) were produced. Expression of the hybrid transgene was essentially observed in pancreas and to a lesser extent in brain. These results indicate that th...

journal_title：Nucleic acids research

authors： Dandoy-Dron F,Monthioux E,Jami J,Bucchini D

更新日期：1991-09-25 00:00:00

abstract：:Augmented biological activity in vitro has been demonstrated in oligonucleotides (oligos) modified to provide nuclease resistance, to enhance cellular uptake or to increase target affinity. How chemical modification affects the duration of effect of an oligo with potent activity has not been investigated directly. We ...

journal_title：Nucleic acids research

authors： Tam RC,Li Y,Noonberg S,Hwang DG,Lui G,Hunt CA,Garovoy MR

更新日期：1994-03-25 00:00:00

abstract：:A newly discovered group of spherical plant viruses contains a bipartite genome consisting of a single-strand linear RNA molecule (RNA 1, Mr 1.5 x 10(6) ), and a single-strand, covalently closed circular viroid-like RNA molecule (RNA 2, Mr approximately 125,000). The nucleotide sequences of the RNA 2 of two of these, ...

journal_title：Nucleic acids research

authors： Haseloff J,Symons RH

更新日期：1982-06-25 00:00:00

abstract：:Translin is a single-stranded RNA- and DNA-binding protein, which has been highly conserved in eukaryotes, from man to Schizosaccharomyces pombe. TRAX is a Translin paralog associated with Translin, which has coevolved with it. We generated structural models of the S. pombe Translin (spTranslin), based on the solved 3...

journal_title：Nucleic acids research

authors： Eliahoo E,Ben Yosef R,Pérez-Cano L,Fernández-Recio J,Glaser F,Manor H

更新日期：2010-05-01 00:00:00

abstract：:DEAD, DEAH and DExH proteins are involved in almost every facet of RNA biochemistry. Members of these protein families exhibit an RNA-dependent ATPase activity and some possess an ATP-dependent RNA helicase activity. Although genetic studies have identified specific functions for certain DEx(D)/(H)proteins from which ...

journal_title：Nucleic acids research

authors： Kossen K,Uhlenbeck OC

更新日期：1999-10-01 00:00:00

abstract：:In order to study the effects of DNA structure on cellular processes such as transcription, we have made a series of plasmids that locate several different kinds of DNA structure (stiff, flexible or curved) near the sites of cleavage by commonly-used restriction enzymes. One can use these plasmids to place any DNA reg...

journal_title：Nucleic acids research

authors： Collis CM,Molloy PL,Both GW,Drew HR

更新日期：1989-11-25 00:00:00

abstract：:Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain dir...

journal_title：Nucleic acids research

authors： Hummel S,Meyerhof W,Korge E,Knöchel W

更新日期：1984-06-25 00:00:00

abstract：:Nuclei isolated from cultured mouse and rat cell lines initiated pre-rRNA chains at the correct site and with the correct nucleotide specificity (A for mouse, G for rat). Nucleic acid filter hybridization and S1 nuclease mapping were used to analyze the RNA products initiated with nucleoside (beta-S)triphosphates. Ini...

journal_title：Nucleic acids research

authors： Zhang ZY,Thompson EA,Stallcup MR

更新日期：1984-11-12 00:00:00

abstract：:A novel cytoplasmic gene expression system has been developed. This system differs from other expression systems in that it relies on the co-delivery of plasmid DNA and T7 RNA polymerase (RNAP) during transfection. The plasmid contains a T7 RNAP gene driven by the T7 promoter (T7 autogene) and a functional/reporter ge...

journal_title：Nucleic acids research

authors： Chen X,Li Y,Xiong K,Wagner TE

更新日期：1994-06-11 00:00:00

abstract：:Highly repetitive Hind III restriction fragments of 0.72-0.76 KBP from total Xenopus laevis genomic DNA are organized in a tandem like arrangement. Cloning of these fragments in pBR 322 with subsequent restriction site mapping and nucleotide sequence analysis of some selected clones showed two different types of seque...

journal_title：Nucleic acids research

authors： Meyerhof W,Tappeser B,Korge E,Knöchel W

更新日期：1983-10-25 00:00:00

abstract：:The FREP database (http://facts.gsc.riken.go.jp/FREP/) contains 31 396 RepeatMasker-identified non-redundant variant repeat sequences derived from 16,527 mouse cDNAs with protein-coding potential. The repeats were computationally associated with potential effects on transcriptional variation, translation, protein func...

journal_title：Nucleic acids research

authors： Nagashima T,Matsuda H,Silva DG,Petrovsky N,Konagaya A,Schönbach C,Kasukawa T,Arakawa T,Carninci P,Kawai J,Hayashizaki Y,RIKEN GER Group.,Genome Science Laoratory Members.

更新日期：2004-01-01 00:00:00

abstract：:The RNA of a deleted strain (lacking Src gene) of an avian sarcoma virus (ASV) was examined by a newly developed immunoelectron microscopic procedure which uses anti-nucleotide antibodies as probes. After denaturation of the RNA and reaction with a high affinity, highly specific anti-7-methylguanosine-5'-phosphate (an...

journal_title：Nucleic acids research

authors： Castleman H,Meredith RD,Erlanger BF

更新日期：1980-10-10 00:00:00

abstract：:Prokaryotes evolved numerous systems that defend against predation by bacteriophages. In addition to well-known restriction-modification and CRISPR-Cas immunity systems, many poorly characterized systems exist. One class of such systems, named BREX, consists of a putative phosphatase, a methyltransferase and four othe...

journal_title：Nucleic acids research

authors： Gordeeva J,Morozova N,Sierro N,Isaev A,Sinkunas T,Tsvetkova K,Matlashov M,Truncaite L,Morgan RD,Ivanov NV,Siksnys V,Zeng L,Severinov K

更新日期：2019-01-10 00:00:00

abstract：:Three Z-DNA-binding proteins of Mr 31, 33 and 58 kD were isolated from mature bull testis. They were obtained in a native state suitable for binding studies. These are the first examples of Z-DNA-binding proteins from a mammalian tissue. Purification involved tissue extraction with 0.35 M NaCl, cation exchange chromat...

journal_title：Nucleic acids research

authors： Gut SH,Bischoff M,Hobi R,Kuenzle CC

更新日期：1987-12-10 00:00:00

abstract：:Human apolipoprotein (apo) B mRNA is edited in a tissue specific reaction, to convert glutamine codon 2153 (CAA) to a stop translation codon. The RNA editing product templates and hybridises as uridine, but the chemical nature of this reaction and the physical identity of the product are unknown. After editing in vitr...

journal_title：Nucleic acids research

authors： Hodges PE,Navaratnam N,Greeve JC,Scott J

更新日期：1991-03-25 00:00:00

abstract：:The RosettaDesign server identifies low energy amino acid sequences for target protein structures (http://rosettadesign.med.unc.edu). The client provides the backbone coordinates of the target structure and specifies which residues to design. The server returns to the client the sequences, coordinates and energies of ...

journal_title：Nucleic acids research

authors： Liu Y,Kuhlman B

更新日期：2006-07-01 00:00:00

abstract：:The interactions of archetypical nucleic acid ligands with the HIV-1 polypurine tract (PPT) RNA:DNA hybrid, as well as analogous DNA:DNA, RNA:RNA and swapped hybrid substrates, were used to probe structural features of the PPT that contribute to its specific recognition and processing by reverse transcriptase (RT). Re...

journal_title：Nucleic acids research

authors： Turner KB,Brinson RG,Yi-Brunozzi HY,Rausch JW,Miller JT,Le Grice SF,Marino JP,Fabris D

更新日期：2008-05-01 00:00:00

abstract：:We describe a novel 3'-OH unblocked reversible terminator with the potential to improve accuracy and read-lengths in next-generation sequencing (NGS) technologies. This terminator is based on 5-hydroxymethyl-2'-deoxyuridine triphosphate (HOMedUTP), a hypermodified nucleotide found naturally in the genomes of numerous ...

journal_title：Nucleic acids research

authors： Litosh VA,Wu W,Stupi BP,Wang J,Morris SE,Hersh MN,Metzker ML

更新日期：2011-03-01 00:00:00

abstract：:It has been proposed that DNA multiply damaged sites (MDS), where more than one moiety in a local region ( approximately 1 helical turn, 10 bp) of the DNA is damaged, are lesions of enhanced biological significance. However, other than indirect measures, there are few analytical techniques that allow direct detection ...

journal_title：Nucleic acids research

authors： Bowman KJ,Pla RL,Guichard Y,Farmer PB,Jones GD

更新日期：2001-10-15 00:00:00

abstract：:Highly conserved sequences present at an identical position near the 3' ends of eukaryotic and prokaryotic 5S rRNAs are complementary to the 5' strand of the m2(6)A hairpin structure near the 3' ends of 18S rRNA and 16S rRNA, respectively. The extent of base-pairing and the calculated stabilities of the hybrids that c...

journal_title：Nucleic acids research

authors： Azad AA

更新日期：1979-12-11 00:00:00

abstract：:The interaction of several synthetic analogues of d-ApA with Poly U and Poly dT was examined to explore the effects of substituents at phosphorus on binding properties of oligonucleotides. These analogues contained a bulky, lipophilic group (2,2,2-trichloroethoxy or 2,2,2-trichloro-1,1-dimethylethoxy) a small, uncharg...

journal_title：Nucleic acids research

authors： Letsinger RL,Bach SA,Eadie JS

更新日期：1986-04-25 00:00:00

abstract：:A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U placed in position U3,...

journal_title：Nucleic acids research

authors： Pasternak A,Hernandez FJ,Rasmussen LM,Vester B,Wengel J

更新日期：2011-02-01 00:00:00

abstract：:31P NMR chemical shifts of the selected mono- and oligonucleotide derivatives, including the compounds with P-N, P-C, P-S bonds and phosphite nucleotide analogues have been presented. The influence of substituents upon 31P chemical shifts has been discussed. The concrete examples of 31P chemical shifts data applicatio...

journal_title：Nucleic acids research

authors： Lebedev AV,Rezvukhin AI

更新日期：1984-07-25 00:00:00

abstract：:In vivo footprinting experiments, augmented with gel shift and transfection analyses suggest that activation of the CYP1A1 gene by aryl hydrocarbons may be a multicomponent process. During the first 30 minutes of exposure to aryl hydrocarbon carcinogens and environmental contaminants, in vivo footprints appear at nine...

journal_title：Nucleic acids research

authors： Robertson RW,Zhang L,Pasco DS,Fagan JB

更新日期：1994-05-11 00:00:00

abstract：:G-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during ...

journal_title：Nucleic acids research

authors： David AP,Margarit E,Domizi P,Banchio C,Armas P,Calcaterra NB

更新日期：2016-05-19 00:00:00

abstract：:From a library of nucleic acid molecules, which are randomized in parts of their sequence, unique sequence variants can be selected for specific properties. The planning of such an in vitro selection experiment requires some consideration regarding how much DNA template or RNA transcript should be used initially. The ...

journal_title：Nucleic acids research

authors： Tabler M,Benos P,Dörr M

更新日期：1996-09-01 00:00:00

abstract：:The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding t...

journal_title：Nucleic acids research

authors： Jacob ST,Rose KM

更新日期：1974-11-01 00:00:00

abstract：:The 2'-5' oligoadenylate synthetases form a well conserved family of interferon induced proteins, presumably present throughout the mammalian class. Using the Expressed Sequence Tag databases, we have identified a novel member of this family. This protein, which we named p59 2'-5' oligoadenylate synthetase-like protei...

journal_title：Nucleic acids research

authors： Hartmann R,Olsen HS,Widder S,Jorgensen R,Justesen J

更新日期：1998-09-15 00:00:00

abstract：:The major immediate early regulatory region of human cytomegalovirus (HCMV) has a complex set of DNA sites through which both cellular and viral factors coordinately regulate immediate early gene expression. In undifferentiated human teratocarcinoma (T2) cells we have previously shown that major immediate early gene e...

journal_title：Nucleic acids research

authors： Kothari S,Baillie J,Sissons JG,Sinclair JH

更新日期：1991-04-25 00:00:00

abstract：:Virulence factor database (VFDB) was set up in 2004 dedicated for providing current knowledge of virulence factors (VFs) from various medical significant bacterial pathogens to facilitate pathogenomic research. Nowadays, complete genome sequences of almost all the major pathogenic microbes have been determined, which ...

journal_title：Nucleic acids research

authors： Yang J,Chen L,Sun L,Yu J,Jin Q

更新日期：2008-01-01 00:00:00