Effects of pendant groups at phosphorus on binding properties of d-ApA analogues.

abstract：:Comprehensive understanding of structure and recognition properties of regulatory nucleic acid elements in real time and atomic level is highly important to devise efficient therapeutic strategies. Here, we report the establishment of an innovative biophysical platform using a dual-app nucleoside analog, which serves ...

journal_title：Nucleic acids research

authors： Nuthanakanti A,Ahmed I,Khatik SY,Saikrishnan K,Srivatsan SG

更新日期：2019-07-09 00:00:00

abstract：:Mouse DNA contains two equally abundant, homologous subfamilies of MspI 3.6 and 5 kb repeated fragments. The first subfamily corresponds to the previously described (1) Bam 4 kb repeats, the second one to Bam repeated fragments of higher molecular weight. These subfamilies account for the vast majority of long Bam rep...

journal_title：Nucleic acids research

authors： Meunier-Rotival M,Bernardi G

更新日期：1984-02-10 00:00:00

abstract：:We have used in vitro transcription in isolated pea nuclei to determine whether the expression of genes for the seed storage proteins legumin and vicilin is regulated transcriptionally or post-transcriptionally in response to changing sulfur status in the developing seed. During the first 48 h of recovery from S defic...

journal_title：Nucleic acids research

authors： Beach LR,Spencer D,Randall PJ,Higgins TJ

更新日期：1985-02-11 00:00:00

abstract：:Reversible methylation of the N6 or N1 position of adenine in RNA has recently been shown to play significant roles in regulating the functions of RNA. RNA can also be alkylated upon exposure to endogenous and exogenous alkylating agents. Here we examined how regio-specific methylation at the hydrogen bonding edge of ...

journal_title：Nucleic acids research

authors： You C,Dai X,Wang Y

更新日期：2017-09-06 00:00:00

abstract：:The increasing availability of multi-omic platforms poses new challenges to data analysis. Joint visualization of multi-omics data is instrumental in better understanding interconnections across molecular layers and in fully utilizing the multi-omic resources available to make biological discoveries. We present here P...

journal_title：Nucleic acids research

authors： Hernández-de-Diego R,Tarazona S,Martínez-Mira C,Balzano-Nogueira L,Furió-Tarí P,Pappas GJ Jr,Conesa A

更新日期：2018-07-02 00:00:00

abstract：:RNA editing of miRNAs, especially in the seed region, adds another layer to miRNA mediated gene regulation which can modify its targets, altering cellular signaling involved in important processes such as differentiation. In this study, we have explored the role of miRNA editing in CD4(+) T cell differentiation. CD4(+...

journal_title：Nucleic acids research

authors： Negi V,Paul D,Das S,Bajpai P,Singh S,Mukhopadhyay A,Agrawal A,Ghosh B

更新日期：2015-09-18 00:00:00

abstract：:The finding in animal species of complexes homologous to the products of six Saccharomyces cerevisiae genes, origin of replication recognition complex (ORC), has suggested that ORC-related mechanisms have been conserved in all eukaryotes. In plants, however, the only cloned putative homologs of ORC subunits are the Ar...

journal_title：Nucleic acids research

authors： Witmer X,Alvarez-Venegas R,San-Miguel P,Danilevskaya O,Avramova Z

更新日期：2003-01-15 00:00:00

abstract：:The POU domain transcription factor, Oct-2, is essential for the B cell-specific expression of CD36 in mouse B cells. In order to determine how Oct-2 mediates expression of CD36 in B cells, we cloned and analysed the mouse CD36 promoter. In contrast to the human CD36 promoter, the mouse promoter contains a consensus o...

journal_title：Nucleic acids research

authors： Shore P,Dietrich W,Corcoran LM

更新日期：2002-04-15 00:00:00

abstract：:Galahad (https://galahad.esat.kuleuven.be) is a web-based application for analysis of drug effects. It provides an intuitive interface to be used by anybody interested in leveraging microarray data to gain insights into the pharmacological effects of a drug, mainly identification of candidate targets, elucidation of m...

journal_title：Nucleic acids research

authors： Laenen G,Ardeshirdavani A,Moreau Y,Thorrez L

更新日期：2015-07-01 00:00:00

abstract：:Lnc2Meth (http://www.bio-bigdata.com/Lnc2Meth/), an interactive resource to identify regulatory relationships between human long non-coding RNAs (lncRNAs) and DNA methylation, is not only a manually curated collection and annotation of experimentally supported lncRNAs-DNA methylation associations but also a platform t...

journal_title：Nucleic acids research

authors： Zhi H,Li X,Wang P,Gao Y,Gao B,Zhou D,Zhang Y,Guo M,Yue M,Shen W,Ning S,Jin L,Li X

更新日期：2018-01-04 00:00:00

abstract：:A soluble enzyme system that specifically initiates lambda dv plasmid DNA replication at a bacteriophage lambda replication origin [Wold et al. (1982) Proc. Natl. Acad. Sci. USA 79, 6176-6180] is also capable of replicating the single-stranded circular chromosomes of phages M13 and phi X174 to a duplex form. This chai...

journal_title：Nucleic acids research

authors： LeBowitz JH,McMacken R

更新日期：1984-04-11 00:00:00

abstract：:Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detection of hybridisatio...

journal_title：Nucleic acids research

authors： Guerasimova A,Ivanov I,Lehrach H

更新日期：1999-01-15 00:00:00

abstract：:The efficiency of Nucleotide Excision Repair (NER)process is crucial for maintaining genomic integrity because in many organisms, including humans, it represents the only system able to repair a wide range of DNA damage. The aim of the work was to investigate whether the efficiency of the repair of photoproducts induc...

journal_title：Nucleic acids research

authors： Bee L,Marini S,Pontarin G,Ferraro P,Costa R,Albrecht U,Celotti L

更新日期：2015-02-27 00:00:00

abstract：:At the DNA/RNA level, biological signals are defined by a combination of spatial structures and sequence motifs. Until now, few attempts had been made in writing general purpose search programs that take into account both sequence and structure criteria. Indeed, the most successful structure scanning programs are usua...

journal_title：Nucleic acids research

authors： Billoud B,Kontic M,Viari A

更新日期：1996-04-15 00:00:00

abstract：:Signals setting the level of transcription of the tRNA-tufB operon have been studied by deletion mapping. TufB transcription was measured in vivo with plasmid-borne tRNA-tufB:galk operon fusions. Removal of the sequences from -133 to -58 with respect to the transcription start point, results in a 90% decrease of tufB ...

journal_title：Nucleic acids research

authors： van Delft JH,Mariñon B,Schmidt DS,Bosch L

更新日期：1987-11-25 00:00:00

abstract：:The E. coli protein StpA has RNA annealing and strand displacement activities and it promotes folding of RNAs by loosening their structures. To understand the mode of action of StpA, we analysed the relationship of its RNA chaperone activity to its RNA-binding properties. For acceleration of annealing of two short RNA...

journal_title：Nucleic acids research

authors： Mayer O,Rajkowitsch L,Lorenz C,Konrat R,Schroeder R

更新日期：2007-01-01 00:00:00

abstract：:Two fragments cloned from purified human satellite III DNA do not cross-react with each other. One fragment, for which a partial sequence is reported, hybridises to satellite II as well as III and is shown to originate on chromosome 1. The other cloned fragment originates from the Y chromosome. This fragment has under...

journal_title：Nucleic acids research

authors： Cooke HJ,Hindley J

更新日期：1979-07-25 00:00:00

abstract：:Ribosome biogenesis is fundamental for cellular life, but surprisingly little is known about the underlying pathway. In eukaryotes a comprehensive collection of experimentally verified ribosome biogenesis factors (RBFs) exists only for Saccharomyces cerevisiae. Far less is known for other fungi, animals or plants, and...

journal_title：Nucleic acids research

authors： Ebersberger I,Simm S,Leisegang MS,Schmitzberger P,Mirus O,von Haeseler A,Bohnsack MT,Schleiff E

更新日期：2014-02-01 00:00:00

abstract：:We characterize a component of the E. coli bacterial nucleoid H1a, which accumulates in stationary phase. This protein, identical with the major component of a plasmid-protein complex previously isolated in our laboratory, has a pI close to 7.5. Acrylamide gel electrophoresis and sedimentation in sucrose gradient have...

journal_title：Nucleic acids research

authors： Spassky A,Rimsky S,Garreau H,Buc H

更新日期：1984-07-11 00:00:00

abstract：:A differential effect is found of various bivalent cations (Ba2+, Ca2+, Mg2+, Cd2+, Co2+, Mn2+, Ni2+, Zn2+ and Hg2+) on stability of intermolecular Py-Pu-Pu triplex with different sequence of base triads. Ca2+, Mg2+, Cd2+, Co2+, Mn2+, Ni2+ and Zn2+ do stabilize the d(C)n d(G)n d(G)n triplex whereas Ba2+ and Hg2+ do no...

journal_title：Nucleic acids research

authors： Malkov VA,Voloshin ON,Soyfer VN,Frank-Kamenetskii MD

更新日期：1993-02-11 00:00:00

abstract：:A screening for human proteins capable of interacting with influenza virus NS1 has been carried out using the two-hybrid genetic trap in yeast. A cDNA corresponding to the human homologue of Drosophila melanogaster Staufen protein (hStaufen) was isolated that fulfilled all genetic controls of the two-hybrid protocol. ...

journal_title：Nucleic acids research

authors： Falcón AM,Fortes P,Marión RM,Beloso A,Ortín J

更新日期：1999-06-01 00:00:00

abstract：:RNA levels in a cell are regulated by the relative rates of RNA synthesis and decay. We recently developed a new approach for measuring both RNA synthesis and decay in a single experimental setting by biosynthetic labeling of newly transcribed RNA. Here, we show that this provides measurements of RNA half-lives from m...

journal_title：Nucleic acids research

authors： Friedel CC,Dölken L,Ruzsics Z,Koszinowski UH,Zimmer R

更新日期：2009-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides, which negatively regulate the gene expression at the post-transcriptional level. This study describes an update of the miRTarBase (http://miRTarBase.mbc.nctu.edu.tw/) that provides information about experimentally validated miRNA-target inte...

journal_title：Nucleic acids research

authors： Chou CH,Chang NW,Shrestha S,Hsu SD,Lin YL,Lee WH,Yang CD,Hong HC,Wei TY,Tu SJ,Tsai TR,Ho SY,Jian TY,Wu HY,Chen PR,Lin NC,Huang HT,Yang TL,Pai CY,Tai CS,Chen WL,Huang CY,Liu CC,Weng SL,Liao KW,Hsu WL,Hu

更新日期：2016-01-04 00:00:00

abstract：:Topoisomerases catalyse changes in the topological state of DNA and are required for many aspects of DNA metabolism. While the functions of topoisomerases I and II in eukaryotes are well established, the role of topoisomerase III remains poorly defined. We have identified a gene in the fission yeast Schizosaccharomyce...

journal_title：Nucleic acids research

authors： Goodwin A,Wang SW,Toda T,Norbury C,Hickson ID

更新日期：1999-10-15 00:00:00

abstract：:Alternative splicing (AS) and alternative polyadenylation (APA) generate diverse transcripts in mammalian genomes during development and differentiation. Epigenetic marks such as trimethylation of histone H3 lysine 36 (H3K36me3) and DNA methylation play a role in generating transcriptome diversity. Intragenic CpG isla...

journal_title：Nucleic acids research

authors： Amante SM,Montibus B,Cowley M,Barkas N,Setiadi J,Saadeh H,Giemza J,Contreras-Castillo S,Fleischanderl K,Schulz R,Oakey RJ

更新日期：2020-09-04 00:00:00

abstract：:1,10-Phenanthroline-platinum(II)-ethylenediamine ( PEPt ) forms a crystalline complex with cytidine-3'-phosphate (3'-CMP) and its structure has been determined by X-ray crystallography. 3'-CMP molecules are hemiprotonated and form hydrogen-bonded pairs that stack above and below the phenanthroline-platinum(II) drug mo...

journal_title：Nucleic acids research

authors： Vijay-Kumar S,Sakore TD,Sobell HM

更新日期：1984-04-25 00:00:00

abstract：:A number of different protecting groups were compared with respect to their usefulness for protection of 2'-hydroxyl functions during synthesis of oligoribonucleotides using the H-phosphonate approach. The comparison was between the t-butyldimethylsilyl (t-BDMSi), the o-chlorobenzoyl (o-CIBz), the tetrahydropyranyl (T...

journal_title：Nucleic acids research

authors： Rozners E,Westman E,Strömberg R

更新日期：1994-01-11 00:00:00

abstract：:The GenBank((R))sequence database incorporates publicly available DNA sequences of >55 000 different organisms, primarily through direct submission of sequence data from individual laboratories and large-scale sequencing projects. Most submissions are made using the BankIt (Web) or Sequin programs and accession number...

journal_title：Nucleic acids research

authors： Benson DA,Karsch-Mizrachi I,Lipman DJ,Ostell J,Rapp BA,Wheeler DL

更新日期：2000-01-01 00:00:00

abstract：:Segments of SV40 DNA having homologous overlapping termini recombine to produce viable genomes in monkey cells. Frequencies of recombination on either side of a deletion marker are non-random; replication and palindromes do not appear to be essential. Since recombination involves host enzymes, a suitable system has be...

journal_title：Nucleic acids research

authors： Upcroft P,Carter B,Kidson C

更新日期：1980-06-25 00:00:00

abstract：:We report the first isolation and characterisation of a human Ig V lambda gene. The gene was isolated from a recombinant phage library of human chromosome 22 using a mouse Ig lambda cDNA as probe. DNA sequence analysis predicts a short leader peptide interrupted by an intron of 88 nucleotides, and a mature polypeptide...

journal_title：Nucleic acids research

authors： Anderson ML,Szajnert MF,Kaplan JC,McColl L,Young BD

更新日期：1984-09-11 00:00:00