A crystalline end product produced by the hydrolytic cleavage of an RNA-like fragment by an organometallointercalator: 1,10-phenanthroline-platinum(II)-ethylenediamine-cytidine 3' monophosphate.

abstract：:The DNA-Actinomycin D interaction has been studied by resonance Raman effect using DNA as chromophore. First, the resonance Raman spectra of DNA obtained with a U.V. excitation at wavelengths of 300 nm and 280 nm are presented. The main Raman hands are assigned to the convenient nucleic bases by comparison with the sp...

journal_title：Nucleic acids research

authors： Chinsky L,Turpin PY

更新日期：1978-08-01 00:00:00

abstract：:An improved contact hybridization technique for the analysis of labelled RNA species is presented. The method combines high sensitivity of detection with the high resolution of polyacrylamide gel electrophoresis and should be especially useful for the characterization of transient RNA precursor molecules. Its applicat...

journal_title：Nucleic acids research

authors： Burckhardt J,Telford J,Birnstiel ML

更新日期：1979-07-11 00:00:00

abstract：:Major efforts are underway to elucidate the spatial distribution of ribosomal RNAs (rRNAs) in the ribosome. An especially informative approach is the identification of likely base-base tertiary interactions within the RNA by phylogenetic comparison. Herein evidence is presented for three heretofore unrecognized candid...

journal_title：Nucleic acids research

authors： Haselman T,Camp DG,Fox GE

更新日期：1989-03-25 00:00:00

abstract：:DNA primase has been partially purified from wheat germ. This enzyme, like DNA primases characterized from many procaryotic and eucaryotic sources, catalyses the synthesis of primers involved in DNA replication. However, the wheat enzyme differs from animal DNA primase in that it is found partially associated with a D...

journal_title：Nucleic acids research

authors： Laquel P,Castroviejo M,Litvak S

更新日期：1990-08-25 00:00:00

abstract：:Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the last 68 residues of the 3'-non-coding region of beet necrotic yellow vein benevirus RNA 3. The structure consists of two stem-loops separated by a single-stranded region. RNA 3-derived transcripts were ...

journal_title：Nucleic acids research

authors： Lauber E,Guilley H,Richards K,Jonard G,Gilmer D

更新日期：1997-12-01 00:00:00

abstract：:The Bovine Genome Database (BGD; http://BovineGenome.org) strives to improve annotation of the bovine genome and to integrate the genome sequence with other genomics data. BGD includes GBrowse genome browsers, the Apollo Annotation Editor, a quantitative trait loci (QTL) viewer, BLAST databases and gene pages. Genome ...

journal_title：Nucleic acids research

authors： Childers CP,Reese JT,Sundaram JP,Vile DC,Dickens CM,Childs KL,Salih H,Bennett AK,Hagen DE,Adelson DL,Elsik CG

更新日期：2011-01-01 00:00:00

abstract：:Next-generation sequencing is excellently suited to evaluate the abundance of mRNAs to study gene expression. Here we compare two alternative technologies, cap analysis of gene expression (CAGE) and serial analysis of gene expression (SAGE), for the same RNA samples. Along with quantifying gene expression levels, CAGE...

journal_title：Nucleic acids research

authors： Hestand MS,Klingenhoff A,Scherf M,Ariyurek Y,Ramos Y,van Workum W,Suzuki M,Werner T,van Ommen GJ,den Dunnen JT,Harbers M,'t Hoen PA

更新日期：2010-09-01 00:00:00

abstract：:Bacteriophage T4 Dda helicase has recently been shown to be active as a monomer for unwinding of short duplex oligonucleotides and for displacing streptavidin from 3'-biotinylated oligonucleotides. However, its activity for streptavidin displacement and DNA unwinding has been shown to increase as the number of Dda mol...

journal_title：Nucleic acids research

authors： Byrd AK,Raney KD

更新日期：2006-05-31 00:00:00

abstract：:UV-irradiation (lambda = 254 nm) of liquid aqueous solutions of deoxyguanosine in the presence of oxygen at pH less than 7 causes an intensive degradation of nucleoside. The quantum yield estimated from A254 decrease for the reaction mixture was found to be 1.5X10(-4) at doses to 150 E/mole. The rate of A254 decrease ...

journal_title：Nucleic acids research

authors： Ivanchenko VA,Budowsky EI,Simukova NA,Vul'fson NS,Tishchenko AI,Askerov DB

更新日期：1977-04-01 00:00:00

abstract：:(19)F NMR spectroscopy has proved to be a valuable tool to monitor functionally important conformational transitions of nucleic acids. Here, we present a systematic investigation on the application of 5-fluoro pyrimidines to probe DNA and RNA secondary structures. Oligonucleotides with the propensity to adapt secondar...

journal_title：Nucleic acids research

authors： Puffer B,Kreutz C,Rieder U,Ebert MO,Konrat R,Micura R

更新日期：2009-12-01 00:00:00

abstract：:Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain dir...

journal_title：Nucleic acids research

authors： Hummel S,Meyerhof W,Korge E,Knöchel W

更新日期：1984-06-25 00:00:00

abstract：:The goal of the present study is to identify genes that respond to iron availability. Suppression subtraction hybridization (SSH) was used to generate cDNA libraries from iron loaded and control human astrocytoma cells (SW1088). The cDNA libraries were screened with antisense cDNA probes obtained from mRNA isolated fr...

journal_title：Nucleic acids research

authors： Ye Z,Connor JR

更新日期：2000-04-15 00:00:00

abstract：:'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base substitutions in the center of the primers. ...

journal_title：Nucleic acids research

authors： Li XM,Shapiro LJ

更新日期：1993-08-11 00:00:00

abstract：:The human 7SL RNA component of the signal recognition particle can be separated into four major conformers by nondenaturing polyacrylamide gel electrophoresis. We have investigated what sequences give 7SL RNA the property to exist in different conformations. The human 7SL gene 7L30.1 was mutagenized using the random l...

journal_title：Nucleic acids research

authors： Zwieb C,Ullu E

更新日期：1986-06-11 00:00:00

abstract：:HinP1I recognizes and cleaves the palindromic tetranucleotide sequence G downward arrowCGC in DNA. We report three structures of HinP1I-DNA complexes: in the presence of Ca(2+) (pre-reactive complex), in the absence of metal ion (binary complex) and in the presence of Mg(2+) (post-reactive complex). HinP1I forms a bac...

journal_title：Nucleic acids research

authors： Horton JR,Zhang X,Maunus R,Yang Z,Wilson GG,Roberts RJ,Cheng X

更新日期：2006-02-09 00:00:00

abstract：:MCScan is an algorithm able to scan multiple genomes or subgenomes in order to identify putative homologous chromosomal regions, and align these regions using genes as anchors. The MCScanX toolkit implements an adjusted MCScan algorithm for detection of synteny and collinearity that extends the original software by in...

journal_title：Nucleic acids research

authors： Wang Y,Tang H,Debarry JD,Tan X,Li J,Wang X,Lee TH,Jin H,Marler B,Guo H,Kissinger JC,Paterson AH

更新日期：2012-04-01 00:00:00

abstract：:Uridylation-dependent RNA decay is a widespread eukaryotic pathway modulating RNA homeostasis. Terminal uridylyltransferases (Tutases) add untemplated uridyl residues to RNA 3'-ends, marking them for degradation by the U-specific exonuclease Dis3L2. In Schizosaccharomyces pombe, Cid1 uridylates a variety of RNAs. In t...

journal_title：Nucleic acids research

authors： Chung CZ,Jaramillo JE,Ellis MJ,Bour DYN,Seidl LE,Jo DHS,Turk MA,Mann MR,Bi Y,Haniford DB,Duennwald ML,Heinemann IU

更新日期：2019-04-08 00:00:00

abstract：:Genes in the germline (micronuclear) genome of hypotrichous ciliates are interrupted by multiple, short, non-coding, AT-rich sequences called internal eliminated segments, or IESs. During conversion of a micronucleus to a somatic nucleus (macronucleus) after cell mating, all IESs are excised from the germline genes an...

journal_title：Nucleic acids research

authors： Prescott DM

更新日期：1999-03-01 00:00:00

abstract：:With the increasing number of protein structures available, there is a need for tools capable of automating the comparison of ensembles of structures, a common requirement in structural biology and bioinformatics. PSSweb is a web server for protein structural statistics. It takes as input an ensemble of PDB files of p...

journal_title：Nucleic acids research

authors： Gaillard T,Stote RH,Dejaegere A

更新日期：2016-07-08 00:00:00

abstract：:The catalytic strategies of small self-cleaving ribozymes often involve interactions between nucleobases and the ribonucleic acid (RNA) backbone. Here we show that multiply protonated, gaseous RNA has an intrinsic preference for the formation of ionic hydrogen bonds between adenine protonated at N3 and the phosphodies...

journal_title：Nucleic acids research

authors： Fuchs E,Falschlunger C,Micura R,Breuker K

更新日期：2019-08-22 00:00:00

abstract：:H3K4me3 is a histone modification that accumulates at the transcription-start site (TSS) of active genes and is known to be important for transcription activation. The way in which H3K4me3 is regulated at TSS and the actual molecular basis of its contribution to transcription remain largely unanswered. To address thes...

journal_title：Nucleic acids research

authors： Lloret-Llinares M,Pérez-Lluch S,Rossell D,Morán T,Ponsa-Cobas J,Auer H,Corominas M,Azorín F

更新日期：2012-10-01 00:00:00

abstract：:Numerous eukaryotic genes are alternatively spliced. Recently, deep transcriptome sequencing has skyrocketed proportion of alternatively spliced genes; over 95% human multi-exon genes are alternatively spliced. One fundamental question is: are all these alternative splicing (AS) events functional? To look into this is...

journal_title：Nucleic acids research

authors： Wang M,Zhang P,Shu Y,Yuan F,Zhang Y,Zhou Y,Jiang M,Zhu Y,Hu L,Kong X,Zhang Z

更新日期：2014-12-16 00:00:00

abstract：:A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cl...

journal_title：Nucleic acids research

authors： Keshet E,Rosner A,Bernstein Y,Gorecki M,Aviv H

更新日期：1981-01-10 00:00:00

abstract：:The transcriptional regulator Spx plays a key role in maintaining the redox homeostasis of Bacillus subtilis cells exposed to disulfide stress. Defects in Spx were previously shown to lead to differential expression of numerous genes but direct and indirect regulatory effects could not be distinguished. Here we identi...

journal_title：Nucleic acids research

authors： Rochat T,Nicolas P,Delumeau O,Rabatinová A,Korelusová J,Leduc A,Bessières P,Dervyn E,Krásny L,Noirot P

更新日期：2012-10-01 00:00:00

abstract：:We have isolated and characterized a S. pombe promoter using a functional heterologous gene product assay. Random S. pombe genomic fragments were cloned upstream from the promoterless 'lacZ gene and tested in vivo for their efficiency to promote expression of the beta-galactosidase protein in the fission yeast. An eff...

journal_title：Nucleic acids research

authors： Kudla B,Persuy MA,Gaillardin C,Heslot H

更新日期：1988-09-12 00:00:00

abstract：:A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing S...

journal_title：Nucleic acids research

authors： Pyle LE,Finch LR

更新日期：1988-07-11 00:00:00

abstract：:The initial genomic response to serum growth factors is the transcriptional activation of a set of immediate-early genes. Serum-induced transcriptional activation of several of these genes involves the formation of a ternary complex that includes the serum response factor (SRF), a 62 kDa ternary complex factor (TCF) a...

journal_title：Nucleic acids research

authors： Latinkić BV,Zeremski M,Lau LF

更新日期：1996-04-01 00:00:00

abstract：:Differences between species have been suggested to largely reside in the network of connections among the genes. Nevertheless, the rate at which these connections evolve has not been properly quantified. Here, we measure the extent to which co-regulation between pairs of genes is conserved over large phylogenetic dist...

journal_title：Nucleic acids research

authors： Snel B,van Noort V,Huynen MA

更新日期：2004-09-07 00:00:00

abstract：:We present a simple and highly accurate computational method for operon prediction, based on intergenic distances and functional relationships between the protein products of contiguous genes, as defined by STRING database (Jensen,L.J., Kuhn,M., Stark,M., Chaffron,S., Creevey,C., Muller,J., Doerks,T., Julien,P., Roth,...

journal_title：Nucleic acids research

authors： Taboada B,Verde C,Merino E

更新日期：2010-07-01 00:00:00

abstract：:We analyzed the effects of site-directed mutations in the SUC2 promoter of Saccharomyces cerevisiae. Analyses were performed in wild-type as well as mig1 and tup1 mutant strains after the promoter mutants were reintroduced into the native SUC2 locus on the left arm of chromosome IX. Mutation of the two GC boxes reveal...

journal_title：Nucleic acids research

authors： Bu Y,Schmidt MC

更新日期：1998-02-15 00:00:00