Comparative sequence and structure of viroid-like RNAs of two plant viruses.

abstract：:Budding yeast telomerase is mainly activated by Tel1/Mec1 (yeast ATM/ATR) on Cdc13 from late S to G2 phase of the cell cycle. Here, we demonstrated that the telomerase-recruitment domain of Cdc13 is also phosphorylated by Cdk1 at the same cell cycle stage as the Tel1/Mec1-dependent regulation. Phosphor-specific gel an...

journal_title：Nucleic acids research

authors： Tseng SF,Shen ZJ,Tsai HJ,Lin YH,Teng SC

更新日期：2009-06-01 00:00:00

abstract：:PRINTS is a database of protein family 'fingerprints' offering a diagnostic resource for newly-determined sequences. By contrast with PROSITE, which uses single consensus expressions to characterise particular families, PRINTS exploits groups of motifs to build characteristic signatures. These signatures offer improve...

journal_title：Nucleic acids research

authors： Attwood TK,Beck ME,Flower DR,Scordis P,Selley JN

更新日期：1998-01-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) resides in a high ROS environment and suffers more mutations than its nuclear counterpart. Increasing evidence suggests that mtDNA mutations are not the results of direct oxidative damage, rather are caused, at least in part, by DNA replication errors. To understand how the mtDNA replicase, P...

journal_title：Nucleic acids research

authors： Anderson AP,Luo X,Russell W,Yin YW

更新日期：2020-01-24 00:00:00

abstract：:Tetramers of the arginine-rich histones H3 and H4 associate with supercoiled SV40 DNA either singly, giving tetrameric nucleoprotein complexes or in pairs giving octameric complexes, both of which are visualized as beads in the electron microscope. The relative amounts of the two complexes may be revealed by complete ...

journal_title：Nucleic acids research

authors： Thomas JO,Oudet P

更新日期：1979-10-10 00:00:00

abstract：:The PRISM web server enables fast and accurate prediction of protein-protein interactions (PPIs). The prediction algorithm is knowledge-based. It combines structural similarity and accounts for evolutionary conservation in the template interfaces. The predicted models are stored in its repository. Given two protein st...

journal_title：Nucleic acids research

authors： Baspinar A,Cukuroglu E,Nussinov R,Keskin O,Gursoy A

更新日期：2014-07-01 00:00:00

abstract：:Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells from all three ger...

journal_title：Nucleic acids research

authors： Park SJ,Komiyama Y,Suemori H,Umezawa A,Nakai K

更新日期：2016-01-04 00:00:00

abstract：:Transcription termination defines accurate transcript 3'-ends and ensures programmed transcriptomes, making it critical to life. However, transcription termination mechanisms remain largely unknown in Archaea. Here, we reported the physiological significance of the newly identified general transcription termination fa...

journal_title：Nucleic acids research

authors： Yue L,Li J,Zhang B,Qi L,Li Z,Zhao F,Li L,Zheng X,Dong X

更新日期：2020-09-25 00:00:00

abstract：:Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Here we report the interesting phenomenon that puromycin acting as a non-inhibitor at very low concentration (e.g. 0.04 microM) can bond only to full-length ...

journal_title：Nucleic acids research

authors： Miyamoto-Sato E,Nemoto N,Kobayashi K,Yanagawa H

更新日期：2000-03-01 00:00:00

abstract：:The DNA replication, plasmid segregation and transactivation functions of Epstein-Barr nuclear antigen 1 (EBNA1) require the binding of EBNA1 to specific DNA recognition sites in the two non-contiguous functional elements of the Epstein-Barr virus latent origin of replication, oriP . EBNA1 molecules bound to these ele...

journal_title：Nucleic acids research

authors： Avolio-Hunter TM,Frappier L

更新日期：1998-10-01 00:00:00

abstract：:A RNA interference (RNAi) like mechanism is involved in elimination of thousands of DNA segments from the developing somatic macronucleus of Tetrahymena, yet how specific internal eliminated sequences (IESs) are recognized remains to be fully elucidated. To define requirements for DNA rearrangement, we performed mutag...

journal_title：Nucleic acids research

authors： Kowalczyk CA,Anderson AM,Arce-Larreta M,Chalker DL

更新日期：2006-01-01 00:00:00

abstract：:Effective drug discovery and optimization can be accelerated by techniques capable of deconvoluting the complexities often present in targeted biological systems. We report a single-molecule approach to study the binding of an alternative splicing regulator, muscleblind-like 1 protein (MBNL1), to (CUG)n = 4,6 and the ...

journal_title：Nucleic acids research

authors： Haghighat Jahromi A,Honda M,Zimmerman SC,Spies M

更新日期：2013-07-01 00:00:00

abstract：:In testis mRNA stability and translation initiation are extensively under the control of poly(A)-binding proteins (PABP). Here we have cloned a new human testis-specific PABP (PABP3) of 631 amino acids (70.1 kDa) with 92.5% identical residues to the ubiquitous PABP1. A northern blot of multiple human tissues hybridise...

journal_title：Nucleic acids research

authors： Féral C,Guellaën G,Pawlak A

更新日期：2001-05-01 00:00:00

abstract：:Transcript of the rbpA1 gene in Anabaena variabilis accumulates significantly at low growth temperatures below 28 degreesC. This accumulation was maximal at 16 degreesC. Accumulation of the rbpA1 transcript was completely abolished by rifampicin, but not by chloramphenicol. Photosynthesis was not required for this col...

journal_title：Nucleic acids research

authors： Sato N,Nakamura A

更新日期：1998-05-01 00:00:00

abstract：:Influenza A virus segment 2 mRNA expresses three polypeptides: PB1, PB1-F2 and PB1-N40, from AUGs 1, 4 and 5 respectively. Two short open reading frames (sORFs) initiated by AUGs 2 and 3 are also present. To understand translational regulation in this system, we systematically mutated AUGs 1-4 and monitored polypeptid...

journal_title：Nucleic acids research

authors： Wise HM,Barbezange C,Jagger BW,Dalton RM,Gog JR,Curran MD,Taubenberger JK,Anderson EC,Digard P

更新日期：2011-09-01 00:00:00

abstract：:The web software SIC provides a tool to search for short inverted segments (length 3-5000 bp) in a DNA sequence. The sequence is assumed to follow a Markov model. A statistic which is sensitive to inversion is presented. Searching inverted segments is done by a scanning approach after the user specifies the size of th...

journal_title：Nucleic acids research

authors： Robelin D,Richard H,Prum B

更新日期：2003-07-01 00:00:00

abstract：:KRAS is one of the most mutated genes in human cancer. It is controlled by a G4 motif located upstream of the transcription start site. In this paper, we demonstrate that 8-oxoguanine (8-oxoG), being more abundant in G4 than in non-G4 regions, is a new player in the regulation of this oncogene. We designed oligonucleo...

journal_title：Nucleic acids research

authors： Cogoi S,Ferino A,Miglietta G,Pedersen EB,Xodo LE

更新日期：2018-01-25 00:00:00

abstract：:Synthetic deoxyoligonucleotides have been 5'-aminoalkylated at the end of step-wise synthesis on the polymer support. This was achieved through the activation of the 5'-hydroxyl group as its 5'-imidazolyl derivative using carbonyldiimidazole, which was subsequently displaced with hexamethylene diamine to yield the tit...

journal_title：Nucleic acids research

authors： Wachter L,Jablonski JA,Ramachandran KL

更新日期：1986-10-24 00:00:00

abstract：:While biallelic mutations in the PALB2 tumor suppressor cause Fanconi anemia subtype FA-N, monoallelic mutations predispose to breast and familial pancreatic cancer. Although hundreds of missense variants in PALB2 have been identified in patients to date, only a few have clear functional and clinical relevance. Herein...

journal_title：Nucleic acids research

authors： Rodrigue A,Margaillan G,Torres Gomes T,Coulombe Y,Montalban G,da Costa E Silva Carvalho S,Milano L,Ducy M,De-Gregoriis G,Dellaire G,Araújo da Silva W Jr,Monteiro AN,Carvalho MA,Simard J,Masson JY

更新日期：2019-11-18 00:00:00

abstract：:GC content bias describes the dependence between fragment count (read coverage) and GC content found in Illumina sequencing data. This bias can dominate the signal of interest for analyses that focus on measuring fragment abundance within a genome, such as copy number estimation (DNA-seq). The bias is not consistent b...

journal_title：Nucleic acids research

authors： Benjamini Y,Speed TP

更新日期：2012-05-01 00:00:00

abstract：:Sequence-specific interactions between aminoacyl-tRNA synthetases and their cognate tRNAs ensure both accurate RNA recognition and the efficient catalysis of aminoacylation. The effects of tRNA(Trp)variants on the aminoacylation reaction catalyzed by wild-type Escherichia coli tryptophanyl-tRNA synthe-tase (TrpRS) hav...

journal_title：Nucleic acids research

authors： Ibba M,Sever S,Praetorius-Ibba M,Söll D

更新日期：1999-09-15 00:00:00

abstract：:N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-...

journal_title：Nucleic acids research

authors： Guigues M,Leng M

更新日期：1979-02-01 00:00:00

abstract：:It is common knowledge that conserved residues evolve slowly. We challenge generality of this central tenet of molecular biology by describing the fast evolution of a conserved nucleotide position that is located in the overlap of two open reading frames (ORFs) of polyomaviruses. The de novo ORF is expressed through e...

journal_title：Nucleic acids research

authors： Lauber C,Kazem S,Kravchenko AA,Feltkamp MC,Gorbalenya AE

更新日期：2015-05-26 00:00:00

abstract：:In most eukaryotes, genetic exchange between paired homologs occurs in the context of a tripartite proteinaceous structure called the synaptonemal complex (SC). Genetic analyses have revealed that the genes encoding SC proteins are vital for meiotic chromosome pairing and recombination. However, the number, nature and...

journal_title：Nucleic acids research

authors： Anuradha S,Muniyappa K

更新日期：2004-04-28 00:00:00

abstract：:Two enzymatic pathways are involved in the inhibitory effects of double-stranded (ds)RNA on protein synthesis in cell extracts derived from interferon-treated human fibroblasts or HeLa cells, an oligonucleotide polymerase that synthesizes (2'-5')An from ATP and a protein kinase that phosphorylates the alpha subunit of...

journal_title：Nucleic acids research

authors： Baglioni C,Minks MA,De Clercq E

更新日期：1981-10-10 00:00:00

abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression post-transcriptionally. Previous studies, which characterized miRNA function, revealed their involvement in fundamental biological processes. Importantly, miRNA expression is deregulated in many human diseases. Specific inhibition of miRNAs usin...

journal_title：Nucleic acids research

authors： Takahashi M,Yamada N,Hatakeyama H,Murata M,Sato Y,Minakawa N,Harashima H,Matsuda A

更新日期：2013-12-01 00:00:00

abstract：:Despite the large number of noncoding RNAs in human genome and their roles in many diseases include cancer, we know very little about them due to lack of structural clues. The centerpiece of the structural clues is the full RNA base-pairing structure of secondary and tertiary contacts that can be precisely obtained on...

journal_title：Nucleic acids research

authors： Zhang Z,Xiong P,Zhang T,Wang J,Zhan J,Zhou Y

更新日期：2020-02-20 00:00:00

abstract：:E2F transcription factors (E2F1-8) are known to coordinately regulate the expression of a plethora of target genes, including those coding for microRNAs (miRNAs), to control cell cycle progression. Recent work has described the atypical E2F factor E2F7 as a transcriptional repressor of cell cycle-related protein-codin...

journal_title：Nucleic acids research

authors： Mitxelena J,Apraiz A,Vallejo-Rodríguez J,Malumbres M,Zubiaga AM

更新日期：2016-07-08 00:00:00

abstract：:Distamycin binds the minor groove of duplex DNA at AT-rich regions and has been a valuable probe of protein interactions with double-stranded DNA. We find that distamycin can also inhibit protein interactions with G-quadruplex (G4) DNA, a stable four-stranded structure in which the repeating unit is a G-quartet. Using...

journal_title：Nucleic acids research

authors： Cocco MJ,Hanakahi LA,Huber MD,Maizels N

更新日期：2003-06-01 00:00:00

abstract：:In order to characterize structure-function relationships, the kinetic behavior of chimeric RNA/DNA ribozyme was compared with that of all RNA ribozyme. Determined kcat values were proven to represent the chemical-cleavage step and not the product-dissociation step. In agreement with the finding by Dahm and Uhlenbeck ...

journal_title：Nucleic acids research

authors： Sawata S,Shimayama T,Komiyama M,Kumar PK,Nishikawa S,Taira K

更新日期：1993-12-11 00:00:00

abstract：:The C (pifC) protein of miniF represses transcription of its own gene by binding to the pif operator (pifO); it is also needed for replication initiated from the miniF primary origin (ori-1). We have determined the nucleotide sequence of the C gene. The gene has been inserted into an expression vector under Ptrp contr...

journal_title：Nucleic acids research

authors： Caughey PA,de Feyter R,Lane HE

更新日期：1986-12-22 00:00:00